Mesencult adipogenic differentiation medium

Manufactured by STEMCELL

Sourced in Canada

Mesencult Adipogenic Differentiation medium is a culture medium designed to support the adipogenic differentiation of mesenchymal stem/stromal cells (MSCs) in vitro. The medium contains a proprietary combination of growth factors and supplements that promote the differentiation of MSCs into adipocytes.

Automatically generated - may contain errors

Lab products found in correlation

Mesencult acf chondrogenic differentiation medium, by STEMCELL (3 mentions) Alcian blue, by Merck Group (1 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions) Alizarin red, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions) Mesencult osteogenic stimulatory kit, by STEMCELL (1 mentions) Osteogenic differentiation medium, by STEMCELL (1 mentions)

Close spelling variants of this product

Adipogenic differentiation medium MesenCult medium

7 protocols using mesencult adipogenic differentiation medium

Characterization of Wharton's Jelly Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The phenotype of WJMSCs was analysed according to the International Society of Cellular Therapy standards. Briefly, cells cultured at passage 3 or 4 were collected and stained with antibodies against CD73, CD90, CD105, CD3, CD45, CD34, CD14 and CD19 (Becton Dickinson) for 30 min at 4°C in darkness. Appropriate isotype controls were used to exclude non‐specific binding. Cells were analysed using Attune Nxt Flow cytometer (Thermo Fisher Scientific, Waltham, MA, USA), and data were analysed using Attune NxT Software v2.2.
WJMSCs were tested for their three‐lineage differentiation potential using MesenCult Adipogenic Differentiation Medium, MesenCult Osteogenic Differentiation and MesenCult ACF Chondrogenic Differentiation Medium (all from StemCell Technologies, Vancouver, CA‐BC, Canada). For analysis, cells were seeded into 12‐well plate at a density of 1.3 × 10³ cells/cm² and cultured in the standard medium until the culture reached appropriate confluence and the medium was replaced by differentiation medium. At the end of differentiation, cell was stained with Oil Red O (adipocytes) (Sigma‐Aldrich) Alizarin Red (MERC) (osteoblasts) and Alcian Blue staining (chondrocytes) (Sigma‐Aldrich) according to standard procedures.

Krzyżak A.T., Habina‐Skrzyniarz I., Mazur W., Sułkowski M., Kot M, & Majka M. (2022). Nuclear magnetic resonance footprint of Wharton Jelly mesenchymal stem cells death mechanisms and distinctive in‐cell biophysical properties in vitro. Journal of Cellular and Molecular Medicine, 26(5), 1501-1514.

+ Open protocol

+ Expand

Adipogenic Differentiation of Pericyte Subpopulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD146⁺ periosteal, adipose, and dermal pericytes or subpopulations based on CXCR4 expression of equal passage number were seeded in 24- or 48-well plates at a density of 2 × 10⁵ cells per mL and allowed to adhere overnight. In total, 24 h after seeding, basal medium was replaced with adipogenic differentiation medium, replenished every 3 days (Mesencult Adipogenic Differentiation medium, StemCell technologies Inc., Vancouver, BC). Oil red O staining was performed after 10–14 days differentiation. Cells were washed with PBS, and fixed with 4% paraformaldehyde for 15 min. After fixation, cells were washed with water and 500 μL Oil red O staining solution. Oil red O stock solution was prepared by dissolving 0.5 g of Oil red O in 100 mL isopropanol. Oil red O staining solution was prepared by dilution of stock solution with distilled water in a 3:2 ratio, followed by filtration. Oil red O staining was performed for 30 min at 37 °C. Following incubation, cells were washed with tap water followed by microscopy. All experiments were performed with n = 3 human samples per anatomic depot, and in triplicate wells (biologic and technical triplicate).

Xu J., Li D., Hsu C.Y., Tian Y., Zhang L., Wang Y., Tower R.J., Chang L., Meyers C.A., Gao Y., Broderick K., Morris C., Hooper J.E., Nimmagadda S., Péault B, & James A.W. (2020). Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues. Bone Research, 8, 22.

+ Open protocol

+ Expand

Defining Multipotent Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To confirm the multipotentiality of MSCs used in our research, experiments were performed in accordance with the minimal criteria for defining multipotent MSCs proposed by the International Society for Cellular Therapy (ISCT) (17 (link)).
The cultured plastic-adherent cells expressing the markers CD73 (sic passim; eBioScience, San Diego, CA, USA), CD90 and CD105 but not expressing the markers CD14, CD19, CD34, CD45 and HLA-DR were able to differentiate into adipocytes, osteoblasts and chondrocyte induced by products of Stem Cell Technologies (Vancouver, BC, Canada), which are respectively MesenCult^™ Adipogenic Differentiation Medium (human) and MesenCult^™ Osteogenic Stimulatory kit (human) and MesenCult^™−ACF Chondrogenic Differentiation Medium. Manufacturer's manuals were referred.
To determine whether the expanded MSC cultures maintained multipotency differentiation characteristics, we tested both HD-MSCs and AD-MSC for differentiation into adipogenic, osteogenic and chondrogenic cell lines. MSCs cultured in adipogenic differentiation medium showing lipid droplets were stained by Oil Red O staining. Osteogenic differentiation was demonstrated by calcium deposition, which was stained by Alizarin Red S. Histological sections of chondrogenic pellet were stained with Alcian Blue and Nuclear Fast Red. Undifferentiated AD-MSCs and HD-MSCs were used as controls.

Yang J., Zou S., Liao M, & Qu L. (2017). Transcriptome sequencing revealed candidate genes relevant to mesenchymal stem cells' role in aortic dissection patients. Molecular Medicine Reports, 17(1), 273-283.

+ Open protocol

+ Expand

Adipogenic Differentiation of MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols

MEFs (6 × 10⁴) were cultured in 24-well plates for 24 hours. Subsequently, the culture medium was replaced with MesenCult adipogenic differentiation medium (StemCell Technologies) for 6 days. Following the differentiation period, cells were fixed using 4% paraformaldehyde for 15 minutes at room temperature. The fixed cells were then stained with Oil Red O for 30 minutes. To quantify the Oil Red O staining, 100% isopropanol was added, and cells were incubated on a shaker for 10 minutes to release Oil Red O from stained cells. The resulting Oil Red O solution in isopropanol (100 μL) was transferred to a 96-well plate and the absorbance measured at 510 nm.

Maharaj A.V., Cottrell E., Thanasupawat T., Joustra S.D., Triggs-Raine B., Fujimoto M., Kant S.G., van der Kaay D., Clement-de Boers A., Brooks A.S., Aguirre G.A., Martín del Estal I., Castilla de Cortázar Larrea M.I., Massoud A., van Duyvenvoorde H.A., De Bruin C., Hwa V., Klonisch T., Hombach-Klonisch S, & Storr H.L. (2024). Characterization of HMGA2 variants expands the spectrum of Silver-Russell syndrome. JCI Insight, 9(6), e169425.

+ Open protocol

+ Expand

Adipogenic Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

PSC were seeded in six well plates at a density of 1 × 10⁵ cells per well and allowed to adhere overnight. Medium was then replaced with Mesencult Adipogenic Differentiation medium (StemCell technologies Inc., Vancouver, BC). Cells were cultured under adipogenic differentiation conditions for fifteen d for gene expression analysis. Differentiation medium was changed every three d. For visualization by Oil Red O staining: After 15 d of adipogenic differentiation, cells were washed with PBS, and fixed with 10% formalin for 30 min. Oil Red O stock solution was prepared from powder (Sigma, St. Louis, MO) by mixing 300 mg Oil Red O powder with 100 ml of 99% isopropanol. Stock solution was diluted 3:2 stock solution: deionized water and allowed to sit at room temperature for 10 min. The working solution was then filtered by gravity filtration. After fixation by formalin, cells were washed with water and 60% isopropanol was added to wells for 5 min before staining. Oil Red O working solution was then added to each well and incubated at 37 °C for 30 min. Following incubation, the cells were washed with tap water images were taken using Q capture software.

Meyers C.A., Xu J., Asatrian G., Ding C., Shen J., Broderick K., Ting K., Soo C., Peault B, & James A.W. (2018). WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells. Scientific Reports, 8, 15618.

+ Open protocol

+ Expand

Adipogenic Differentiation of CD146+ Pericytes

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD146⁺ adipose pericytes from 3 to 8 passages were seeded in 12-well plates at a density of 2 × 10⁵ cells per well and allowed to adhere overnight. At 24 hours after seeding, the basal medium was replaced with adipogenic differentiation medium and replenished every 3 days (Mesencult Adipogenic Differentiation medium, StemCell Technologies Inc.). Oil Red O staining was performed after 10 days of differentiation (4 (link), 40 (link), 42 (link)). Cells were washed with PBS and fixed with 4% formaldehyde for 15 minutes. After fixation, cells were washed with water and 500 μL of Oil Red O staining solution. Oil Red O stock solution was prepared by dissolving 0.5 g of Oil Red O in 100 mL isopropanol. Oil Red O staining solution was prepared by dilution of a stock solution with distilled water in a 3:2 ratio, followed by filtration. Oil Red O staining was performed for 30 minutes at 37°C. Following incubation, cells were washed with tap water, followed by microscopy. After imaging, Oil Red O stain was extracted with 100% isopropanol for 5 minutes followed by an absorbance at 548 nm for quantification. All experiments were performed with n = 3 human samples per anatomic depot and in triplicate wells (biologic and technical triplicate).

Hsu G.C., Wang Y., Lu A.Z., Gomez-Salazar M.A., Xu J., Li D., Meyers C., Negri S., Wangsiricharoen S., Broderick K., Peault B., Morris C, & James A.W. (2023). TIAM1 acts as an actin organization regulator to control adipose tissue–derived pericyte cell fate. JCI Insight, 8(13), e159141.

+ Open protocol

+ Expand

Adipogenic, Osteogenic, and Chondrogenic Differentiation of ADSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

ADSCs cultured in DMEM/F12 medium to 90%–100% confluent were further cultured in MesenCult™ Adipogenic Differentiation Medium or Osteogenic Differentiation Medium (STEMCELL Technologies, Vancouver, Canada) for 9–10 days. The cells were fixed with 4% paraformaldehyde and stained with oil red O or alizarin red S to identify adipogenic and osteogenic differentiation, respectively.
To induce chondrogenic differentiation, ADSC pellet was incubated with MesenCult™ ACF Chondrogenic Differentiation Medium (STEMCELL Technologies, Vancouver, Canada) for 21 days. The pellets were fixed in 10% formalin and embedded with paraffin and thereafter stained with Alcian blue.

Liu Z., Yang Y., Ju J., Zhang G., Zhang P., Ji P., Jin Q., Cao G., Zuo R., Wang H., Yu C., Zhang Z., Le Y., Fu Y, & Hou R. (2022). miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways. Stem Cells International, 2022, 1474273.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!