LNCaP, PC-3, and C4-2 human prostate cancer cell lines, and RWPE1, a human prostate epithelial cell line, were purchased from GeneChem (Shanghai, China). Keratinocyte serum free medium (K-SFM, Gibco, NY, USA) containing calf pituitary extract and EGF was used to culture RWPE1 cells, and Dulbecco’s modified eagle medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Cellmax, Beijing, China) and 1% penicillin-streptomycin (Cellmax) was used to culture LNCaP, C4-2, and PC-3 cells. All cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
Penicillin streptomycin
Manufactured by Cellmax
Sourced in China
Penicillin-streptomycin is a commonly used antibiotic solution composed of penicillin and streptomycin. It is a broad-spectrum antibiotic that is used to prevent bacterial contamination in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Cellmax (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Rwpe 1, by Thermo Fisher Scientific (1 mentions)
Lncap, by Thermo Fisher Scientific (1 mentions)
Lncap, by Genechem (1 mentions)
Du145, by Genechem (1 mentions)
Du145, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
3 protocols using penicillin streptomycin
1
Culturing Human Prostate Cell Lines
2
Cultivation of Human Prostate Cancer Cells
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Human prostate cancer cell lines LNCaP, C4-2, PC-3, and DU145 were purchased from GeneChem (Shanghai, China). LNCaP, DU145, C4-2 and PC-3 cells were cultured in Dulbecco’s modified eagle medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Cellmax, Beijing, China), 1% penicillin-streptomycin (Cellmax) at 37°C in a humidified atmosphere of 5% CO2.
3
Jag1 and Notch1 Overexpression in hADSCs and HaCaT Cells
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The hADSCs were obtained as previously described. The HaCAT cells were obtained from ATCC and cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% FBS (Cellmax) and 1% penicillin/streptomycin (Cellmax) at 37℃ in 5% CO 2 atmosphere. The coding region of Jag1 and Notch1
were constructed by GenePharma (Shanghai, China) and the si-RNA targets sequences speci c for the human Notch1, Jag1 were purchased from GenePharma (Shanghai, China). Cells were cultured in a 6well culture with 50%-60% con uence, starved in medium without FBS for 16h and then transfections of hADSCs and HaCAT cells with the related plasmids were performed with Lipofectamine 2000(Invitrogen) according to the manufacturer's instructions.
were constructed by GenePharma (Shanghai, China) and the si-RNA targets sequences speci c for the human Notch1, Jag1 were purchased from GenePharma (Shanghai, China). Cells were cultured in a 6well culture with 50%-60% con uence, starved in medium without FBS for 16h and then transfections of hADSCs and HaCAT cells with the related plasmids were performed with Lipofectamine 2000(Invitrogen) according to the manufacturer's instructions.
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