Mouse myeloperoxidase elisa kit

Obtained liver tissues were cut into pieces and homogenated on ice with an ultrasonic probe in radioimmunoprecipitation (RIPA) buffer (Solarbio) with 1 mM protein inhibitor. Protein concentrations were measured using a BCA assay kit. Lysates were centrifugated at 10,000 × g for 5 min at 4°C according to the instructions, and the suspension was collected and stored in −80°C for further use. Before tests, a pre-experiment was conducted to optimize the concentration. ELISAs were conducted to measure the following cytokines according to the instructions (mouse TNF-α ELISA kit, mouse IFN-γ ELISA kit, mouse IL-1β ELISA kit, mouse IL-6 ELISA kit, mouse IL-12 ELISA kit, and mouse myeloperoxidase ELISA kit, Solarbio).

About 100 mg frozen lung was homogenized with 1 mL saline, and centrifuged at 12,000 rpm for 10 minutes at 4 °C. The supernatant was used for MPO activity analysis according to the instruction of Mouse Myeloperoxidase ELISA Kit (Solarbio, Beijing, China).