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Cd11c pe cy5

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The CD11c-PE-Cy5 is a fluorescent-labeled antibody used to identify and quantify CD11c-positive cells in flow cytometry applications. CD11c is a common marker for dendritic cells and other myeloid cells. The PE-Cy5 conjugate enables simultaneous detection of the labeled cells.

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Lab products found in correlation

Facs lysing solution, by BD (1 mentions) Cellfix, by BD (1 mentions) Cytometric bead array human inflammatory cytokines kit, by BD (1 mentions) Cd80 fitc, by BD (1 mentions) Cd86 fitc, by BD (1 mentions) Flow cytometry staining buffer, by Thermo Fisher Scientific (1 mentions) Permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Cd25 pe, by Thermo Fisher Scientific (1 mentions) Brefeldin a, by Thermo Fisher Scientific (1 mentions) Anti mouse cd3 pe, by Thermo Fisher Scientific (1 mentions) Anti mouse il 10 pe, by Thermo Fisher Scientific (1 mentions) Cd206 pe, by Thermo Fisher Scientific (1 mentions) Cd40 pe, by Thermo Fisher Scientific (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) F4 80 fitc, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Cd45 pe cy7, by BD (1 mentions) Cd11b apc cy7, by BD (1 mentions) Deoxyribonuclease 1, by Merck Group (1 mentions)

Close spelling variants of this product

CD11c-PE (37 citations) Anti-CD11c-PE-Cy5.5 (2 citations)

3 protocols using cd11c pe cy5

1

Multiparametric Flow Cytometry Analyses

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The following monoclonal antibodies were obtained from Invitrogen (Molecular Probes, Eugene, OR, USA): CD14 TRI-COLOR conjugate and human CD16-fluorescein isothiocyanate (FITC). Fluorescence-activated cell sorting (FACS) lysing solution and CellFIX were purchased from Becton Dickinson Pharmingen (San Diego, CA, USA), along with other monoclonal antibodies: anti-human CD40-FITC, CD80-FITC, CD83-FITC, CD86-FITC, and human leukocyte antigen D-related (HLA-DR) phycoerythrin (PE). Human Hematopoietic Lineage APC Cocktail, anti-human CD123-FITC, and CD11c-PE-Cy5 were obtained from e-Bioscience (San Diego, CA, USA). Finally, the Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit was obtained from BD Biosciences (San Diego, CA, USA).
Esquius L., Javierre C., Llaudó I., Rama I., Oviedo G.R., Massip-Salcedo M., Aguilar-Martínez A., Niño O, & Lloberas N. (2021). Impact of Olive Oil Supplement Intake on Dendritic Cell Maturation after Strenuous Physical Exercise: A Preliminary Study. International Journal of Environmental Research and Public Health, 18(8), 4128.
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2

Multiparametric Flow Cytometry Analysis

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Cells of MLN (5 × 105) were washed in PBS + 1% BSA and stained with anti-mouse CD3-PE, CD4 FITC, CD8 PE, F4/80 FITC, CD40 PE, CD206 PE, CD25 PE, CD11c PE-Cy5, NK FITC, B220 FITC antibodies (eBioscience) in FlowCytometry Staining Buffer (eBioscience) for 1 h at 4 °C in the dark. After repeated washing in PBS + 1%BSA, cells were resuspended in PBS and immediately detected by CyFlow Space (Partec, Görlitz, Germany) and analysed by FlowMax software (Partec). Prior to intracellular cytokine staining, cells (1 × 106) were stimulated with phorbol myristate acetate (PMA, 100 ng/ml) and ionomycin (400 ng/ml) (both from Sigma-Aldrich) in the presence of Brefeldin A (eBioscience) (5 µM) for 4 h, stained with anti-mouse CD5 FITC and anti-mouse CD19 PE-Cy5 (eBioscience) antibody, fixed in 2% paraformaldehyde, permeabilized with Permeabilization buffer (eBioscience) and then stained for the intracellular cytokines with the anti-mouse IL-10 PE (eBioscience).
Vujicic M., Saksida T., Despotovic S., Bajic S.S., Lalić I., Koprivica I., Gajic D., Golic N., Tolinacki M, & Stojanovic I. (2018). The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier. Scientific Reports, 8, 6337.
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3

Ovarian Cell Isolation and Characterization

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Single cell suspensions of ovary cells were prepared by modifying a method previously described (Oakley et al., 2010 (link)). Ovaries were collected from WT and Esr2-PgrKO mice at 6 and 9 h after hCG injection and dissociated by 2 mL of collagenase digestion solution containing 3.5 U of collagenase type I (17100–017; Invitrogen), 1000 U of deoxyribonuclease I (D4527; Sigma), and 40 mg of BSA (017K0723; Sigma) in H-199 media (12350–039; Life Technologies, Inc.) at 37°C for 30 min. Dissociated cells (1 × 106 cells) were washed and stained according to the manufacturer’s protocol with the following antibodies alone or in varying combinations: CD16/CD32 (Mouse BD Fc Block; 553142, BD Biosciences, San Jose, CA) CD45-PE/Cy7 (552848, BD Biosciences), CD11b-APC/Cy7 (557657, BD Biosciences), CD11c-PE/Cy5 (15-0114-82, eBioscience, San Diego, CA), I-A/I-E (MHCII)-FITC (562009, BD Biosciences), Ly6G-Horizon V450 (560603, BD Biosciences), Ly6C-Brilliant Violet 450 (128033, Biolegend, San Diego, CA), CD45R/B220-PE (553089, BD Biosciences), and CD8a-APC (553035, BD Biosciences). For surface marker staining, DPBS (pH 7.4) containing heat-inactivated FBS and < 0.09% sodium azide (554656, BD Biosciences) was used as staining buffer. Stained samples were analyzed by flow cytometry (BD LSR II; BD Biosciences). Data analysis was performed using FCS Express 6 (De Novo Software, Los Angeles, CA).
Park C.J., Lin P.C., Zhou S., Barakat R., Bashir S.T., Choi J.M., Cacioppo J.A., Oakley O.R., Duffy D.M., Lydon J.P, & Ko C.J. (2020). Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation. Cell reports, 31(2), 107496.
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