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Mvx 10 epifluorescence microscope

Manufactured by Olympus
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Sourced in Japan

The Olympus MVX-10 is an epifluorescence microscope. It is designed for imaging and analysis of fluorescently-labeled samples.

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Lab products found in correlation

Two photon microscope, by Bruker (3 mentions) Insight ds, by Spectra-Physics (3 mentions) Na water immersion objective, by Nikon (2 mentions) Acetylated tubulin antibody, by Merck Group (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) 0.8 na water immersion objective, by Nikon (1 mentions)

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Epifluorescence microscope (122 citations) MVX microscope (5 citations)

5 protocols using mvx 10 epifluorescence microscope

1

Mesoscale and Two-Photon Imaging Techniques

Cited 1 time
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Mesoscale imaging (MVX-10 epifluorescence microscope; Olympus, Tokyo Japan) was performed to visualize stimulation-induced activity on a large spatial scale (3.7 × 3 mm) [24 (link), 29 , 36 (link)]. Time series images were captured at 20 frames per second (fps) using a charge-coupled device (CCD) camera (Retiga, R1 18 imaging). A two-photon microscope (Bruker, Madison, WI) equipped with an optical parametric oscillator (OPO) laser (Insight DS+, Spectra Physics, Menlo Park, CA) at 920 nm and a 16 × 0.8 numerical aperture (NA) water immersion objective (Nikon, Melville, NY) was employed to answer questions related to the different temporal calcium dynamics with cellular resolution (407 × 407 μm, 0.8 μm pixel−1; 30 fps).
Stieger K.C., Eles J.R., Ludwig K.A, & Kozai T.D. (2022). Intracortical microstimulation pulse waveform and frequency recruits distinct spatiotemporal patterns of cortical neuron and neuropil activation. Journal of neural engineering, 19(2), 10.1088/1741-2552/ac5bf5.
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2

Immunohistochemistry of Zebrafish Heads

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Natural matings from adult zebrafish homozygote male and heterozygote females (to produce zygotic mutants) and homozygote female and heterozygote males (to produce maternal-zygotic mutants) were raised to 4dpf at 28.5ºC in standard conditions. Embryos were fixed in PBS-buffered 4% paraformaldehyde with 4% sucrose, bleached in 3% hydrogen peroxide, dehydrated in methanol and stored before use. Immunohistochemistry on undissected heads was carried out as described41 (link). Briefly: following rehydration and permeabilisation with proteinase K, embryos were incubated in antibodies against acetylated tubulin antibody (Sigma, 1:1,000) and SV2 (DSBH 1:500) overnight at 4 °C. Following washes, staining was performed with Alexa Fluor 488 nm or 633 nm subtype-specific secondaries (Invitrogen) at 1:200, together with DAPI for background anatomical context. Embryos were initially examined using an Olympus MVX10 epifluorescence microscope and 12 from each cross were selected for mounting and imaging. Confocal imaging employed sequential laser illumination on a Leica SP8 confocal microscope. Images were minimally processed using ImageJ (max projection only). Following imaging, genotyping was carried out as described above.
Bryant D., Seda M., Peskett E., Maurer C., Pomeranz G., Ghosh M., Hawkins T.A., Cleak J., Datta S., Hariri H., Eckert K.M., Jafree D.J., Walsh C., Demetriou C., Ishida M., Alemán-Charlet C., Vestito L., Seselgyte R., McDonald J.G., Bitner-Glindzicz M., Hemberger M., Rihel J., Teboul L., Henne W.M., Jenkins D., Moore G.E, & Stanier P. (2020). Diverse species-specific phenotypic consequences of loss of function sorting nexin 14 mutations. Scientific Reports, 10, 13763.
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3

Mesoscale Imaging Visualizes Neuronal Responses

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Mesoscale imaging was performed during stimulation to visualize a3.7 mm × 3.0 mm field of view (the entire craniotomy). This was done with an MVX-10 epifluorescence microscope (Olympus, Tokyo, Japan) and a CCD camera with a 50 ms exposure time (Retiga R1, QI Imaging). All other imaging was performed with a two-photon microscope that provided superior XYZ resolution (Bruker, Madison, WI). The microscope used an OPO laser (Insight DS+; Spectra Physics; Menlo Park, CA) that was tuned to a 920 nm wavelength and kept below 40 mW throughout the study to prevent tissue burning. All imaging was performed with a 16 × 0.8 NA water immersion objective (Nikon Instruments; Melville, NY) with a 3 mm working distance. Resonance scanning mode was used to collect images at 30 Hz in a 407 × 407 μm2 ROI (512 × 512 pixels). Image collection was triggered by the TDT system and included 10s baseline and 20s post-stimulation image collection periods.
Eles J.R, & Kozai T.D. (2020). In vivo imaging of calcium and glutamate responses to intracortical microstimulation reveals distinct temporal responses of the neuropil and somatic compartments in layer II/III neurons. Biomaterials, 234, 119767.
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4

Mesoscale Fluorescence Imaging of Neuronal Activity

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Mesoscale imaging was performed during stimulation over a 3.7 × 3.0 mm window using an MVX-10 epifluorescence microscope (Olympus, Tokyo, Japan) and a CCD camera with a 50-ms exposure time (Retiga R1, QI imaging) at 20 Hz. For cellular resolution, a two-photon microscope (Bruker, Madison, WI) with an OPO laser (Insight DS+, Spectra Physics, Menlo Park, CA) tuned to 920 nm and kept below 40 mW was used for neuronal and neuropil imaging at 30 Hz using resonance mode. The microscope was equipped with a 16× 0.8 NA water immersion objective (Nikon Instruments, Melville, NY) with a 3 mm working distance resulting FOV of 407 × 407 μm2 (512 × 512 pixels). Image acquisition was initiated by a trigger from the TDT system.
Stieger K.C., Eles J.R., Ludwig K.A, & Kozai T.D. (2020). In vivo microstimulation with cathodic and anodic asymmetric waveforms modulates spatiotemporal calcium dynamics in cortical neuropil and pyramidal neurons of male mice. Journal of neuroscience research, 98(10), 2072-2095.
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5

Large-Scale Mesoscale and Cellular Calcium Imaging

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Mesoscale imaging (MVX-10 epifluorescence microscope; Olympus, Tokyo Japan) was performed to visualize stimulation-induced activity on a large spatial scale (3.7 x 3mm) (24, 36, 37) . Time series images were captured at 20 frames per second (fps) using a CCD camera (Retiga, R1 18 imaging). A two-photon microscope (Bruker, Madison, WI) equipped with an OPO laser (Insight DS+, Spectra Physics, Menlo Park, CA) at 920 nm and a 16x 0.8NA water immersion objective (Nikon, Melville, NY) was employed to answer questions related to the different temporal calcium dynamics with cellular resolution (407 x 407 µm, 0.8 µm/pixel; 30 fps).
Stieger K.C., Eles J.R., Ludwig K.A., & Kozai T.D. (2022). Intracortical microstimulation pulse waveform and frequency recruits distinct spatiotemporal patterns of cortical neuron and neuropil activation.
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