Sds page 4 12 criterion gels

Manufactured by Bio-Rad

SDS–PAGE 4–12% criterion gels are pre-cast polyacrylamide gels designed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). They provide a range of 4-12% acrylamide concentration for the separation of proteins with a wide range of molecular weights.

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Lab products found in correlation

Enhanced chemiluminescence detection kit, by Cytiva (1 mentions) α tubulin, by Merck Group (1 mentions) Hspd1, by Enzo Life Sciences (1 mentions) Hspa2, by Proteintech (1 mentions) Hspb7, by Abcam (1 mentions) Hspa4, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

SDS Page 4–12% gel Criterion SDS-PAGE gels SDS–PAGE (12% gels) SDS-PAGE gel (12%) SDS-PAGE gels Criterion gels PAGE gels SDS-PAGE 10% SDS gel

2 protocols using sds page 4 12 criterion gels

Mechanical Tissue Lysis and Protein Separation

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EHTs were processed by mechanical tissue lysing as described before.^{28 (link)} Proteins were separated on precast SDS–PAGE 4–12% criterion gels (Bio-Rad) and electrotransferred to polyvinylidene difluoride membranes.

Wijnker P.J., Dinani R., van der Laan N.C., Algül S., Knollmann B.C., Verkerk A.O., Remme C.A., Zuurbier C.J., Kuster D.W, & van der Velden J. (2024). Hypertrophic cardiomyopathy dysfunction mimicked in human engineered heart tissue and improved by sodium–glucose cotransporter 2 inhibitors. Cardiovascular Research, 120(3), 301-317.

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Western Blot Analysis of Protein Levels

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For analysis of protein levels by Western blot, whole tissue lysates were used from either the proteomic analysis or were prepared as described previously (34 (link)). Proteins were separated on precast SDS-PAGE 4–12% criterion gels (Bio-Rad) and transferred to polyvinylidene difluoride or nitrocellulose membranes. Site-specific antibodies directed to acetylated α-tubulin (Sigma, T7451), HSPA1 (Enzo Life Sciences, ADI-SPA-810), HSPA2 (Proteintech group, 66291-1), HSPB1 (Enzo Life Sciences, ADI-SPA-800), HSPB5 (Enzo Life Sciences, ADI-SPA-223), HSPB7 (abcam, ab150390), HSPD1 (Enzo Life Sciences, ADI-SPA-805), HSPA4 (Cell Signaling, 3303S), HSP90 (Cell Signaling, 4874S), α-tubulin (Sigma, T9026), tyrosinated tubulin (Sigma, T9028), detyrosinated tubulin (abcam, ab48389), and GAPDH (Cell Signaling, 2118S; Fitzgerald, 10R-G109a) were used to detect the proteins which were visualized with an enhanced chemiluminescence detection kit (Amersham) and scanned with Amersham Imager 600. Protein levels were determined by densitometric analysis and normalized to GAPDH.

Schuldt M., Dorsch L.M., Knol J.C., Pham T.V., Schelfhorst T., Piersma S.R., dos Remedios C., Michels M., Jimenez C.R., Kuster D.W, & van der Velden J. (2021). Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy. Frontiers in Cardiovascular Medicine, 8, 612215.

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