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Microphot fxa microscope

Manufactured by National Instruments

The Microphot FXA microscope is a high-performance optical microscope designed for laboratory use. It features a sturdy, ergonomic design and provides clear, detailed images for a variety of applications. The Microphot FXA is capable of magnifying specimens up to 1000x, making it suitable for a range of research and analysis tasks.

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2 protocols using microphot fxa microscope

1

Microscopic Imaging of Lipid Staining

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A Nikon Microphot FXA microscope equipped with a DS-Fi1 camera and the NIS Elements D.32 imaging software (Nikon instruments Europe B.V., Badhoevedorp, The Netherlands) and a Leica multispectral confocal laser microscope (Leica TCS NT, Heidelberg, Germany) were used to examine the cryosections. Oil red O-stained lipids were detected under the confocal microscope using an excitation laser line at 543 nm (helium-neon). Optical sections (1.0 µm) were acquired, and images were produced using an 8-fold frame averaging a 1024 × 1024 pixel resolution. Representative images were presented using Adobe Creative Cloud (Adobe Inc., San Jose, CA, USA).
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2

Immunofluorescence and Histological Imaging

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IHC and H&E staining were examined with a Nikon MICROPHOT-FXA microscope and images were captured using NIS-Elements D3.10 software. Fluorescent images were captured using a Nikon A1 scanning confocal microscope on an Eclipse Ti microscope stand (Nikon Instruments, Melville, NY). Standard lasers and filters were used to image DAPI, AlexaFluor 488, and TRITC. Maximum intensity projections are shown.
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