Liver cancer cell lines PLC/PRF/5, SMMC-7721, HCC-LM3 and Huh7 were purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences. Cell lines were maintained at 37°C in an atmosphere containing 5% CO2 in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. For in vitro study, PLC/PRF/5, HCC-LM3 were transfected with siRNA (Biotend, Shanghai, People's Republic of China) against NEU1 to knock down NEU1, and SMMC-7721, HuH7 were transfected with pcDNA-NEU1(GenePharma, Shanghai, People's Republic of China)to overexpress NEU1 using Lipofectamine2000 transfection reagent (Invitrogen, USA) according to the manufacturer's instructions. siRNA-control and pcDNA were used as a negative control.
Smmc7721
Manufactured by GenePharma
Sourced in China
The SMMC7721 is a laboratory equipment product. It is a cell line used for research purposes. The core function of the SMMC7721 is to serve as a model system for the study of liver cancer.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by GenePharma (2 mentions)
Smmc 7721, by National Collection of Authenticated Cell Cultures (1 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (1 mentions)
Plc prf 5, by National Collection of Authenticated Cell Cultures (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Hep3b, by GenePharma (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Smmc 7721, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Bel 7404, by American Type Culture Collection (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Anti mir nc, by GenePharma (1 mentions)
3 protocols using smmc7721
1
Modulating Liver Cancer Cell Lines
2
Human HCC Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3
Transfection of HCC cell lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCC cell lines (Huh7, SMMC-7721, HepG2, Hep3B, and BEL-7404) and immortalized normal liver cell line LO2 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cells were maintained with RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and fostered in a humidified incubator containing 5% CO2. When growing to 70–80% confluence, SMMC7721 and HepG2 cells were transfected with miR-137 mimics (miR-137), miR-137 antagomir (anti-miR-137), respective negative controls (miR-NC, anti-miR-NC), or miR-137 + Lv-DNMT3a (GenePharma Shanghai, China) with Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA). The transfected cells were harvested for further analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!