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Plko shrna plasmids

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PLKO.shRNA plasmids are lentiviral vector-based plasmids designed for the expression of short hairpin RNA (shRNA) sequences. These plasmids provide a platform for gene silencing studies by enabling the delivery and stable integration of shRNA constructs into target cells.

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Lab products found in correlation

Pspax2 12260, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions)

Spelling variants of this product

ShRNA plasmids (15 citations) PLKO shRNAs (2 citations) MISSION pLKO.1-shRNA plasmids (2 citations) ShRNA lentiviral plasmids (pLKO.1-puro) (2 citations)

2 protocols using plko shrna plasmids

1

Knockdown of TNS4 and GFP in Cells

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pLKO.shRNA plasmids targeting TNS4 and GFP were purchased from Sigma (St. Louis, CA, USA) and produced using protocols from the RNAi Consortium (http://www.broadinstitue.org/rnai/trc). Cells were prepared a day before infection and then incubated for 24 h with dilute virus containing media with 8 μg/mL polybrene. The transfected cells were treated with puromycin for 1 week.
Kim S., Kim N., Kang K., Kim W., Won J, & Cho J. (2019). Whole Transcriptome Analysis Identifies TNS4 as a Key Effector of Cetuximab and a Regulator of the Oncogenic Activity of KRAS Mutant Colorectal Cancer Cell Lines. Cells, 8(8), 878.
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2

Lentiviral Transduction of BMSCs

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The pLKO shRNA plasmids were purchased from Sigma-Aldrich (St. Louis, MO). To generate lentiviral particles, we transiently transfected 293T cells with pLKO vectors with psPAX2 (12260) and pMD2.G (12259) from Addgene. Culture supernatant was collected after 48 h and used to transduce freshly isolated BMSCs for 48 h. After infection, medium was changed to α-MEM supplemented with 10% heat-inactivated FBS and 5% human platelet lysate.
Hu T., Kitano A., Luu V., Dawson B., Hoegenauer K.A., Lee B.H, & Nakada D. (2019). Bmi1 Suppresses Adipogenesis in the Hematopoietic Stem Cell Niche. Stem Cell Reports, 13(3), 545-558.
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