The primary antibodies to Drp1, phospho-Drp1 (Ser616), p53, and cleaved-caspase 3 were purchased from Cell Signaling Technology (CST, MA, USA). Phospho-Drp1 (Ser637), ABCG2 (ATP-binding cassette sub-family G member 2), and Oct4 (octamer-binding transcription factor 4), ALDH1 (aldehyde dehydrogenase 1), and BAX (Bcl-2-associated X protein) antibodies were purchased from Ruiyingbio (Jiangsu, China). Mfn2 antibody was obtained from Abgent (NJ, USA). The antibody against β-actin was from Boster (Wuhan, China). The antibodies against COXIV, CD133, CD44, BMI1 (B lymphoma Mo-MLV insertion region 1 homolog), and Bcl-2 (B-cell lymphoma 2) were from Beyotime (Shanghai, China). LC3 antibody was purchased from Novus Biologicals (CO, USA). The peroxidase-conjugated affinipure secondary antibodies goat anti-rabbit IgG and anti-mouse IgG were purchased from Thermo. Alexa Fluor® 488-labeled goat anti-rabbit IgG and DyLight® 405-labeled goat anti-mouse IgG were obtained from Beyotime.
Antibody against β actin
Manufactured by Boster Bio
Sourced in China
The Antibody against β-actin is a laboratory reagent used to detect and quantify the presence of the β-actin protein in biological samples. β-actin is a ubiquitous and highly expressed cytoskeletal protein found in all eukaryotic cells. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and measure the levels of β-actin in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Bcl 2, by Beyotime (1 mentions)
Dylight 405 labeled goat anti mouse igg, by Beyotime (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Lc3 antibody, by Novus Biologicals (1 mentions)
Alexa fluor 488 labeled goat anti rabbit igg, by Beyotime (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Cell light brdu dna cell proliferation kit, by RiboBio (1 mentions)
Agarose, by Merck Group (1 mentions)
Giemsa stain, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Metformin, by Merck Group (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Selumetinib, by Selleck Chemicals (1 mentions)
Glut1, by Abcam (1 mentions)
Cb 839, by Selleck Chemicals (1 mentions)
Caspase 9, by Abcam (1 mentions)
Bradford protein assay, by Bio-Rad (1 mentions)
P akt, by Abcam (1 mentions)
6 protocols using antibody against β actin
1
Antibody Panel for Cell Signaling
2
Synergistic Anti-Cancer Effects of Metformin and Tamoxifen
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Metformin (1,1-dimethylbiguanide hydrochloride), tamoxifen (4-hydroxytamoxifen), thiazolyl blue tetrazolium bromide (MTT), Giemsa stain, dimethyl sulfoxide (DMSO) and agarose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Metformin was dissolved in sterile water to make a 1 M stock solution, and tamoxifen was dissolved in ethanol to make a 3.2 mM stock solution. The Cell-LightTM BrdU DNA Cell Proliferation Kit was purchased from Ribobio (Guangzhou, China). The antibodies against p-AMPK (Thr172), AMPK, p-mTOR (Ser2448), mTOR, p-p70S6 (Thr389), p70S6, bcl-2 and bax are rabbit monoclonals and were purchased from Cell Signaling Technologies (Beverly, MA, USA). The antibody against β-actin is a mouse monoclonal and was purchased from Boster (Wuhan, China).
3
Metabolic Regulation in Cancer Cells
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CB839 and selumetinib (AZD6244) were purchased from Selleckchem (Houston, TX, USA). For cellular studies, drugs were dissolved in dimethyl sulfoxide (DMSO), and for xenograft experiments, drugs were dissolved in 10% CMC—Na solution. The antibodies against GLS, Glut1, PKM2, MEK, ERK1/2, p-ERKT202+204, AKT, p-AKTs472–474were obtained from Abcam (Cambridge, UK). The antibodies against P62, p-ULK1s555 were obtained from Cell Signaling Technology (Danvers, MA, USA). The antibody against β-actin was obtained from Boster Biological Technology (Wuhan, China).
4
Protein Expression Analysis in PC12 Cells
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The PC12 cells were stimulated for equivalent periods of time and lysed in extraction buffer (10 mM Tris [pH 7.4], 150 mM NaCl, 1% Triton X-100, 5 mM EDTA [pH 8.0]). The protein samples were separated by SDS-PAGE (10%) and electro-transferred onto a nitrocellulose (NC) membrane (Boster Biotechnology Co., Ltd., China). Protein expression was evaluated using antibodies against P53, Bax, Bcl-2, NGFR and α-syn (Boster Biotechnology Co., Ltd., China). The blots were incubated with the appropriate secondary antibodies conjugated to alkaline phosphatase (AP) peroxidase (Boster Biological Technology Co., Ltd., China). The protein levels were normalized by re-probing the blots with antibody against β-actin (Boster Biological Technology Co., Ltd., China) as shown in Figure 2 e,f.
5
MBT Modulates Cell Signaling Pathways
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The HXO-RB44 cells were first seeded onto 6-well plates (106 cells/well) and then treated with MBT at 0, 1, 10, and 20 μM for 24 h. Total cell lysates were obtained after treatment with RIPA buffer and protease inhibitors. The protein concentrations were determined by Bradford protein assay (BioRad Lab., USA). Approximately 75 μg of lysate was resolved on 12% SDS-PAGE, electrotransferred to PVDF membranes (Dingguo, China), and then incubated with specific primary rabbit polyclonal antibodies to cyclin B1, p27, and caspase-3 at 4°C overnight. Caspase-9, LC3-I LC3-II, p-PI3K, and p-Akt were purchased from Abcam, Shanghai, China. Antibody against β-actin and peroxidase-labeled anti-rabbit immunoglobulin were purchased from Boster (China) and an enhanced chemiluminescence (ECL) kit was purchased from Pierce (USA).
6
Inflammatory Response in Porcine Intestinal Cells
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The intestinal porcine epithelial cell line (IPEC-J2) was purchased from CCTCC (Wuhan, China). Dimethyl sulfoxide (DMSO) and lipopolysaccharide (LPS) (Escherichia coli 055:B5) were purchased from Sigma-Aldrich (Oakville, Ontario, Canada). Fetal bovine serum (FBS) and DMEM/F12 medium were obtained from Gibco (Grand Island, NY). ELISA kits for IL-1, IL-6 and TNF-α were obtained from R&D (Minneapolis, MN, USA). Antibodies against p65 and p-p65 were purchased from Cell Signaling Technology Inc.(Shanghai, China). Antibody against β-actin and the secondary antibody of goat anti-rabbit IgG were obtained from Boster Biological Technology Inc.(Wuhan, China). Pyrrolidinedithiocarbamic acid (PDTC, inhibitor of NF-κB activation) were purchased from Beyotime Biotechnology Inc. (Shanghai, China). Methanol (MS1922-801, HPLC grade) and water (WS2211-001, HPLC grade) were purchased from TEDIA (Fairfield, USA); Acetonitrile (ACN) (1.00030.4000, HPLC grade) was purchased from MERCK (Darmstadt, Germany); Formic acid (94318-250 mL-F, MS grade) was purchased from SIGMA (Deisenhofen, Germany); Ammonium formate (65929-0025, LCMS grade) was purchased from ROE SCIENTIFIC INC (Newark, US); Phenylalanine-2,3,4,5,6-d5 (D-5466, 99.7 atom % D) was purchased from CDN isotopes (Canada).
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