Internally-quenched fluorescent (IQF) peptides containing an o-aminobenzoic acid (ABZ) group and a C-terminal 3-nitrotyrosine (Y[3NO2]) were synthesised by GL Biochem at >90% purity and were quantitated by amino acid analysis. These peptides were ABZ-STRNGLPS-Y-(3NO2) and GLPVFAEFLPLFSKFGSRMHILK(K-ABZ)STRNGLPS-Y(3NO2). The control fluorescent peptide ABZ-STRN was also synthesised by GL Biochem at >90% purity and quantitated by amino acid analysis. All IQF or fluorescent peptides were solubilised in 25% (v/v) acetonitrile:water. The caspase inhibitor Ac-YVAD-CMK (where Ac, acetyl; CMK, chloromethylketone) was supplied by Peptides International and solubilised in dimethyl sulfoxide (DMSO) to 10 mM. All R1 and IK8 peptide variants were supplied by GL Biochem at >85% purity, dissolved in ultrapure water prior to analysis and quantitated using the Direct Detect system (Milllipore) according to the manufacturer’s instructions.
Ac yvad cmk
Manufactured by Peptide Institute
Sourced in Japan
Ac-YVAD-CMK is a synthetic peptide that functions as a caspase-1 inhibitor. It serves as a tool for research purposes, enabling the investigation of the role of caspase-1 in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharide lps, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Imiquimod r837, by InvivoGen (1 mentions)
Ruxolitinib, by Cayman Chemical (1 mentions)
Wp1066, by Cayman Chemical (1 mentions)
Nigericin, by Fujifilm (1 mentions)
Ag490, by Cayman Chemical (1 mentions)
2 protocols using ac yvad cmk
1
Quantitation of Fluorescent Peptides
2
Screening of Small Molecule Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Nigericin was purchased from Wako Chemical (Osaka, Japan). R837/imiquimod was purchased from InvivoGen (San Diego, CA, USA) and Tokyo Chemical Industry (Tokyo, Japan). WP1066, AG490 and ruxolitinib were purchased from Cayman Chemical (Ann Arbor, MI, USA). Lipopolysaccharide (LPS) and phorbol 12‐myristate 13‐acetate (PMA) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Ac‐YVAD‐CMK was purchased from Peptide Institute (Osaka, Japan). Chemical compounds in the SCADS Inhibitor Kit (Screening Committee of Anticancer Drugs supported by Grant‐in‐Aid for Scientific Research on Priority Area “Cancer” from the Ministry of Education, Culture, Sports, Science and Technology, Japan) were used for screening.
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