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Verios 5 uc

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Verios 5 UC is a high-resolution scanning electron microscope (SEM) designed for advanced materials characterization. It features an ultra-high-resolution column with field emission gun technology, enabling detailed imaging and analysis of a wide range of samples at the nanoscale level.

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5 protocols using verios 5 uc

1

Characterization of Functionalized Paper-Based AuNPs

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The structures and surface morphologies of the functionalized
papers image were obtained using SEM (Thermo Fisher Scientific, Verios
5 UC) after platinum coating for 120 s. Additionally, energy-dispersive
spectroscopy and elemental mapping (EDS, Oxford Instruments, AztecLive,
and Ultim Max 65) were used in conjunction with SEM to determine the
major atoms (C, O, Si, and Au) and atomic percentage of Paper_AuNPs_CIN.
Moreover, ICP-OES was used to determine the wt % of Si and Au loading
(ICP-OES, Thermo Scientific, iCAP7400DUO). The surface atomic composition
was investigated using X-ray photoelectron spectroscopy (XPS) (Thermo
Scientific, NEXSA). To gain further insight into the chemical bonding
situation, the samples were analyzed using an FT-IR spectrometer equipped
with an attenuated total reflector (ATR) mode, in the range of 4000–400
cm–1 with a resolution of 4.0 and a scan rate of
32 scans. Additionally, the optical properties of the fabricated papers
were monitored between 200 and 800 nm by DRS (PerkinElmer, LAMBDA
950).
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2

Structural Characterization of Carbon Aerogels

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The morphology of carbon sponge aerogels was studied with a field-emission scanning electron microscope (FE-SEM, Gemini 500, ZEISS, Jena, Germany) of the Center for Research Facilities at the Chonnam National University and ultra-high-resolution FE-SEM (Verios 5 UC, Thermo Fisher Scientific, Waltham, WA, USA). The N2 adsorption and desorption isotherm curves of carbon aerogel were obtained on BELSORP-max (MicrotracBEL Corp, Osaka, Japan), and Brunauer-Emmett-Teller (BET) surface area and pore size distribution was calculated based on the non-linear density functional theory (NLDFT) model. The detailed morphology and nanostructure of carbon aerogels was observed with transmission electron microscopy (TEM, JEM-2100F, JEOL, Tokyo, Japan) and aberration-corrected high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM, JEM ARM 200F, JEOL, Tokyo, Japan). X-ray diffraction (XRD) was carried out with X’Pert PRO diffractometer. X-ray photoelectron spectroscopy (XPS) was conducted with K-ALPHA (Thermofisher, Waltham, WA, USA). Raman spectrums were obtained by a laser Raman spectrophotometer (NRS-5100).
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3

Cryo-Cutting Technique for Hollow Fiber Characterization

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The morphology of DHFs and a CHF membrane were characterized by using an ultra-high resolution field emission scanning electron microscope (UHR-FE-SEM, Verios 5 UC, Thermo Fisher Scientific Inc., Waltham, MA, USA). The specimens were prepared via the cryo-cutting method with liquid nitrogen (DAEDEOKGAS Co., Ltd., Incheon, Korea). The HFs were wet with ethyl alcohol (ethanol absolute, 99.9%, Ducksan, Seoul, Korea) and immersed in liquid nitrogen for rapid cooling. By bending frozen HFs, a hollow structure cross-section was obtained. Samples were vertically aligned beside the specimen stage and coated with Pt for 180 s to take the cross-sectional image. Pt coating for 60 s was applied to the lumen and shell sides of HFs by aligning the sample horizontally on the specimen stage.
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4

Validating Nanoparticles Using UHR FE-SEM

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The ultrahigh-resolution field-emission scanning electron microscope (UHR FE-SEM, Verios 5 UC, ThermoFisher, Waltham, MA, USA) was utilized to validate the existence of NPs so that OM raw image was matched to the SEM image. Because the surfaces of GT resonator and NPs are made of SiO2, 3 nm thick platinum was plated on the samples using high-vacuum sputter coater (EM ACE600, Leica, Wetzlar, Germany). All SEM micrographs were obtained at an acceleration voltage of 10 kV with a beam current of 25 pA.
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5

Thermal and Structural Analysis of Amorphous Powders

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The thermal properties of amorphous powders were analyzed using differential scanning calorimetry (DSC, Netzsch DSC 404 F3) at a heating rate of 20 K/min, and the onsets of glass transition temperature (Tg) and crystallization temperature (Tx) were identified by the tangent method. The structures of powders were examined by X-ray diffraction (XRD, Bruker D8 Discover) using Cu-Kα radiation. The morphology was characterized by scanning electron microscopy (SEM, Thermo Fisher Verios 5UC). Furthermore, the structural features at the nanoscale were investigated by a spherical aberration-corrected transmission electron microscopy (TEM, Thermo Fisher Spectra 300). A focused ion beam (FIB, Thermo Fisher Helios 5UX) was used to prepare the TEM samples. Further energy-dispersive X-ray spectroscopy (EDS) analysis was conducted using Thermo Fisher Scientific’s Super-X windowless EDS detector at an acceleration voltage of 300 kV. Electron energy loss spectroscopy (EELS) was used to analyze the hydrogen in the sample.
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