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2 protocols using glacial acetic acid 99.8

1

Reagent Specifications for Chemical Analysis

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The reagents used in the analysis satisfied all quality requirements and were of analytical grade. The following reagents were used in the study: ethanol 96% (v/v) (manufactured by Stumbras AB, Kaunas, Lithuania), the Folin–Ciocalteu reagent (Sigma-Aldrich Chemie, Steinheim, Germany), sodium carbonate (Na2CO3) (Carl Roth GmbH, Karlsruhe, Germany), gallic acid monohydrate (Sigma-Aldrich Chemie, Steinheim, Germany), 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric chloride hexahydrate (FeCl3·6H2O), sodium acetate trihydrate (CH3COONa·3H2O) (Sigma-Aldrich Chemie, Steinheim, Germany), glacial acetic acid 99.8% (Lachner, Neratovice, Czech Republic), 2,4,6-tripyridyl-s-triazine (TPTZ) (Carl Roth GmbH, Karlsruhe, Germany), concentrated hydrochloric acid (Fluka-Chemie, Buchs, Switzerland), Trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromano-2-carboxylic acid) (Sigma-Aldrich, St. Louis, MO, USA), aluminum chloride hexahydrate (Fluka, Germany), hexamethylenetetramine (Sigma-Aldrich, Gillingham, UK), rutin (Carl Roth GmbH, Karlsruhe, Germany), sodium molybdate, sodium nitrite, sodium hydroxide (Chempur, Tarnowskie Gory, Poland); quercitrin, (+)-catechin, and (−)-epicatechin (Sigma-Aldrich, Steinheim, Germany). Purified water was produced using the Milli-Q® water purification system (Millipore, Bedford, MA, USA).
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2

Antioxidant Potential Evaluation

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The reagents used in the assays met all quality requirements and were of analytical grade. The following reagents were used in the study: ethanol 96% (v/v) (manufactured by SC Vilniaus degtinė, Vilnius, Lithuania), Folin–Ciocalteu reagent, gallic acid monohydrate, ferric chloride hexahydrate (FeCl3 × 6H2O), sodium acetate trihydrate (CH3COONa × 3H2O), DPPH (2,2-diphenyl-1-picrylhydrazyl), Trolox ((±) -6-hydroxy-2,5,7,8-tetramethylchromano-2-carboxylic acid), hexamethylenetetramine, chlorogenic acid, caffeic acid, phloridzin, quercetin, quercitrin, kaempferol-3-O-glycoside (+)-catechin, (−)-epicatechin, and (−)-epicatechin gallate (Sigma-Aldrich, Steinheim, Germany), sodium carbonate (Na2CO3), rutin (Carl Roth GmbH, Karlsruhe, Germany), glacial acetic acid 99.8% (Lachner, Neratovice, Czech Republic), 2,4,6-tripyridyl-s-triazine (TPTZ) (Alfa Aesar, Karlsruhe, Germany), concentrated hydrochloric acid, aluminum chloride hexahydrate (Fluka-Chemie, Buchs, Switzerland), sodium molybdate, sodium nitrite, and sodium hydroxide (Chempur, Tarnowskie Gory, Poland). Purified water was prepared, using the Milli-Q® water-purification system (Millipore Co., Bedford, MA, USA).
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