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4 protocols using oxalic acid

1

Fatty Acid Methyl Ester Analysis

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Fatty acid methyl esters (Supelco 35 Component FAME Mix) were obtained from Sigma-Aldrich SL (Madrid, Spain). N-hexane, isopropanol, anhydrous sodium sulfate, undecanoic acid (C11:0), methyl acetate, sodium methoxide, methanol, oxalic acid, and diethyl ether were acquired from Panreac Química SL (Panreac AppliChem, Barcelona, Spain) and Sigma-Aldrich (Merck, Munich, Germany). All reagents were of analytical grade.
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2

Analytical Reagents and Solvents Protocol

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Unless otherwise indicated, all chemicals and solvents were purchased from Merck (Madrid, Spain). L-Ascorbic acid was obtained from Labkem (Barcelona, Spain). Aluminum chloride and sodium carbonate were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Sodium nitrite, sodium hydroxide, and oxalic acid were purchased from Panreac (Barcelona, Spain). Water was purified using a Milli-Q system (Millipore, Burlington, MA, USA). All the chemicals used, including the solvents, were of analytical grade.
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3

Quantification of Phytochemicals in Foods

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All the chemicals and solvents used were of analytical grade. Oxalic acid, fumaric acid, tartaric acid, malic acid, citric acid, lactic acid, D-(+)-fructose, D-(+)-glucose, sucrose, gallic acid 1hydrate, Folin-Ciocalteu reagent, sodium dihydrogen phosphate, sodium carbonate anhydrous, HPLC-grade acetonitrile and 37% (v/v) chloridric acid were purchased from Panreac Applichem (IWT group, Barcelona, Spain). Methanol and anhydrous di-sodium hydrogen phosphate were supplied by Scharlau Chemie (Scharlab S.L. Barcelona, Spain). Thermostable amyloglucosidase (GOPOD) reagent buffer, GOPOD reagent enzymes, D-(+)-glucose standard solution, amyloglucosidase and pancreatic-α-amylase were purchased from Megazyme International (Ireland). Fluorescein, 2, 2-azobis (2-amidinopropane) dihydrochloride (AAPH) and 6-hydroxy-2, 5, 7, 8-tetramethylchromane-2-carboxylic acid (Trolox) were obtained from Acros Organics (Thermo Fisher Scientific Inc. Waltham, MA, USA).
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4

Doxycycline Hyclate Extraction and Quantification

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Doxycycline (as hyclate) powder was supplied by BIOVET (HydroDoxx ® 500 mg/g, Bulgaria). Analytical grade DC hyclate standard for high-performance liquid chromatography (HPLC) was purchased from Sigma Aldrich (St. Louis, MO, USA), and the solid phase extraction (SPE) cartridges (COOH, 500 mg in 3 mL syringe tube) were purchased from J. T. Baker (Center Valley, PA, USA). The acetonitrile (ACN), ethyl acetate (EA), methanol (MeOH) (Echo Chemical, Toufen, Taiwan), hydrochloric acid, sodium hydroxide, phosphoric acid (Union Acta Veterinaria Hungarica 64, 2016 Chemical Works, Hsinchu, Taiwan), oxalic acid, and trichloroacetic acid (Panreac Applichem, Castellar del Vallés, Spain) were all analytical grade.
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