Uv 2100

Manufactured by Shimadzu

Sourced in Japan, United States

The UV-2100 is a UV-Visible spectrophotometer manufactured by Shimadzu. It is a compact and versatile instrument designed for accurate absorbance measurements in the ultraviolet and visible light ranges.

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Lab products found in correlation

Libra 200fe, by Zeiss (1 mentions) Cary eclipse g9800a, by Agilent Technologies (1 mentions) Tga dsc analyzer, by Mettler Toledo (1 mentions) Uv vis spectrophotometer, by Shimadzu (1 mentions) Eclipse plus c18 column, by Agilent Technologies (1 mentions) Aa 670, by Shimadzu (1 mentions) Rx 5000, by Atago (1 mentions)

Spelling variants of this product

UV-2100 spectrophotometer (37 citations) UV-2100 spectrometer (3 citations) UV 2100 UV-VIS Recording Spectrophotometer (3 citations) 2100 UV–Vis spectrophotometer (2 citations) UV 2100 151PC (2 citations) UV-2100 UV/VIS Spectrophotometer (2 citations)

18 protocols using uv 2100

Antioxidant Evaluation of Garlic Juice

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The total polyphenol content was determined using Folin-Ciocalteu’s colorimetric method with some modifications as described in a previous report (Kim et al., 2010 (link)) and was expressed as mg of gallic acid equivalents (GAE). Total flavonoid content was measured using aluminum chloride colorimetric assays as previously reported (Kamtekar et al., 2014 ) and expressed as mg of rutin equivalents (RE).
The free radical scavenging activity of garlic juice was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) assays. For ABTS assays, 2,2′-azobis dihydrochloride (1 mM) and ABTS (2.5 mM) were dissolved in phosphate-buffered saline (100 mM), heated at 70°C for 30 min, and then cooled to room temperature. The diluted ABTS solution (0.98 mL) was then mixed with sample solution (0.02 mL), and the absorbance was measured at 734 nm after 20 min using a spectrophotometer (UV 2100, Shimadzu Co., Kyoto, Japan). For DPPH assays, 1 mM of DPPH was dissolved in ethanol, and 1.95 mL of DPPH solution was mixed with 50 μL of sample solution. The mixture was incubated at room temperature for 30 min, and then the absorbance was measured at 517 nm. The ABTS and DPPH radical scavenging activities were expressed as vitamin C equivalents.

Kim J.H., Kim J.W., Yu S.H., Lee J., Cho H.T., Heo W., Park S.J., Lee J.H, & Kim Y.J. (2019). Utilization of Pectinase Cocktail and High Hydrostatic Pressure for the Production of Aged Black Garlic Juice with Improved Nutritional Value. Preventive Nutrition and Food Science, 24(3), 357-362.

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Antioxidant Enzyme Activity Assays

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Superoxide dismutase (SOD) and catalase (CAT) activities were measured in erythrocytes and plasma, glutathione peroxidase (GPx) and glutathione reductase (GR) activities were determined in erythrocytes, and myeloperoxidase (MPO) activity was determined in plasma by methods previously described [28 (link)]. CAT activity was determined by a spectrophotometric method based on the decomposition of H₂O₂. SOD activity was determined using a xanthine/xanthine oxidase system to produce the superoxide anion. The produced anion induces the reduction of cytochrome C, which was monitored at 550 nm. GPx activity was determined by an assay that requires H₂O₂ and NADPH as substrates and GR as enzyme indicator. The decrease in NADPH absorbance was followed at 340 nm during the oxidation of NADPH to NADP⁺. GR activity determines the rate of conversion of oxidized glutathione (GSSG) to reduced glutathione (GSH) by monitoring the oxidation of NADPH at 340 nm. MPO activity was measured following the oxidation of guaiacol. The reaction mixture contained 13.5 mM guaiacol and the reaction was initiated with 300 μM H₂O₂, and the absorbance at 470 nm was monitored. All enzymatic activities were determined at 37°C in a spectrophotometer Shimadzu UV-2100.

Sureda A., Batle J.M., Martorell M., Capó X., Tejada S., Tur J.A, & Pons A. (2016). Antioxidant Response of Chronic Wounds to Hyperbaric Oxygen Therapy. PLoS ONE, 11(9), e0163371.

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Chlorophyll and UV-B Absorbing Compounds Extraction

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The method for the measure of the concentration of chl a and chl b was extracted by acetone and determined following the reported methods [23 ]. Intact leaf samples of seedlings (fresh weight 0.5 g), which were at 5-6 leaves stage of development, were placed in a mortar and followed by the addition of silica of 0.2 g, CaCO₃ of 0.2 g, and 15 mL 80% acetone. After thorough grinding, the samples were filtrated with two layers of filter paper by pump air and fixed to 25 mL with 80% acetone, and then the absorbance at 663 and 645 nm was determined, respectively. Chlorophyll concentration was calculated and expressed as mg/g FW.
Fresh samples of 0.5 g were taken from the epicotyls and extracted in 10 mL acidified methanol (methanol-water-hydrochloric acid, 79 : 20 : 1, v/v) for UV-B absorbing compounds, according to the procedure of Mirecki and Teramura [24 (link)]. The hydrochloric acid was 36% HCl. Extract absorbance at 300 nm was measured with a spectrophotometer (UV-2100; Shimadzu, Columbia, MD, USA) and the absorbance was arbitrarily used for analysis.

Li F.M., Lu Z.G, & Yue M. (2014). Analysis of Photosynthetic Characteristics and UV-B Absorbing Compounds in Mung Bean Using UV-B and Red LED Radiation. Journal of Analytical Methods in Chemistry, 2014, 378242.

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Oxidative Stress and Inflammation Biomarkers

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As oxidative stress biomarkers, superoxide dismutase (SOD) and catalase (CAT) enzymatic activity were measured in the plasma. Moreover, as proinflammatory cytokines, interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNFα) were determined in the plasma. Specifically, CAT activity was measured by the spectrophotometric method of Aebi based on the decomposition of H₂O₂ [30 (link)], whereas SOD activity was analysed using an adaption of the method of McCord and Fridovich [31 (link)]. Both enzymatic activities were determined using a spectrophotometer Shimadzu UV-2100 (Shimadzu Corporation, Kyoto, Japan) at 37 °C. TNFα and IL-6 were measured in the plasma using Human Custom ProxartPlex^TM (Invitrogen—Thermo Fisher Scientific, Vienna, Austria) following the provided instructions.

Monserrat-Mesquida M., Quetglas-Llabrés M.M., Bouzas C., Pastor O., Ugarriza L., Llompart I., Cevallos-Ibarra K., Sureda A, & Tur J.A. (2023). Plasma Fatty Acid Composition, Oxidative and Inflammatory Status, and Adherence to the Mediterranean Diet of Patients with Non-Alcoholic Fatty Liver Disease. Antioxidants, 12(8), 1554.

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X-ray Characterization of Cs4Mn1-xCuxSb2Cl12 Microcrystals

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X‐ray diffraction (XRD) spectra of Cs₄Mn_1‐xCu_xSb₂Cl₁₂ (x = 0, 0.1, 0.2, 0.3, 0.4, and 0.5) microcrystals were determined by Cu Kα diffraction (MADZU, Japan). The apparent morphology of Cs₄Mn_1‐xCu_xSb₂Cl₁₂ microcrystals was characterized by scanning electron microscopy (SEM, TM4000Plus II). The transmission electron microscopy (TEM) and high‐resolution transmission electron microscopy (HRTEM) were performed on ZEISS LIBRA 200FE. Element mappings were tested by energy dispersive X‐ray spectroscopy (EDX) on the same instrument. Inductively coupled plasma‐atomic emission spectrometry (ICP‐AES) was tested on an Agilent 730. The UV‐visible absorption spectra were determined by the scan UV–vis spectrophotometer (UV‐2100) (Shimadzu, Japan) with a range of 300 to 800 nm.The chemical states and valence bands of Cs₄Mn_1‐xCu_xSb₂Cl₁₂ microcrystals were characterized by X‐ray photoelectron spectroscopy (XPS, ESRCALAB250Xi, Thermo Fisher Scientific). The binding energy referred to the C 1s peak at the binding energy of 284.80 eV. Thermogravimetric analysis (TGA) studies were performed in AR environments from room temperature to 1000 °C using a TGA/DSC analyzer (METTLER TOLEDO, and Switzerland). Time‐resolved photoluminescence (TRPL) spectra were measured using a photoluminescence spectrometer (Cary Eclipse G9800A, Agilent Technologies).

Gao B., Tian C., Guo L., Zhou J., Wang Z., Fu C., Ran H., Chen W., Huang Q., Wu D., Tang X, & Luo Z. (2023). Copper Modulated Lead‐Free Cs4MnSb2Cl12 Double Perovskite Microcrystals for Photocatalytic Reduction of CO2. Advanced Science, 11(6), 2307543.

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Photodegradation of Methylene Blue using Cu@CuS

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The photodegradation efficiency of methylene blue (MB) in aqueous solution was measured under sunlight irradiation (300 W xenon lamp). All experiments were carried out at the temperature of 25 ± 2 °C. Typically, 15 mg of Cu@CuS yolk–shell sample was dispersed in 50 mL of MB aqueous solution (0.02 M) under ultrasonic irradiation to form a suspension, which was magnetically stirred for 30 min in the dark. At regular irradiation time intervals, the dispersion was sampled and centrifuged to separate the residue. The photodegradation efficiency was monitored by measuring the absorbance of the centrifuged solutions at the maximum absorption wavelength of 664 nm using UV–Vis spectroscopy (SHIMADZU, UV-2100) at room temperature.

Li Q., Wang F., Sun L., Jiang Z., Ye T., Chen M., Bai Q., Wang C, & Han X. (2017). Design and Synthesis of Cu@CuS Yolk–Shell Structures with Enhanced Photocatalytic Activity. Nano-Micro Letters, 9(3), 35.

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Plasma Hydrogen Sulfide Quantification

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Plasma H₂S concentrations were measured using the sulfide-sensitive electrode method (PXS-270, Shanghai, China) as described by Sun et al. [6 (link)].
A total of 0.1 mL plasma was added to a tube containing 0.5 mL 1% zinc acetate and 2.5 mL distilled water. Into this tube, 0.5 mL of 20 mmol/L N,N-dimethyl-p-phenylenediamine dihydrochloride in 7.2 mmol/L HCl and 0.4 mL of 30 mmol/L FeCl₃ in 1.2 mmol/L HCl were added and incubated for 20 min at room temperature (RT). The plasma protein was removed by the addition of 1 mL of 10% trichloroacetic acid to the reaction mixture and centrifugation at 3000 r/min for 15 min. The optical absorbance of the resulting solution was measured at 670 nm using a spectrometer (Shimadzu UV 2100; Shimadzu, Kyoto, Japan).
All samples were assayed in duplicate, and the concentration in the solution was calculated against a calibration curve of NaHS (3.125-250 mmol/L).

Teng Y., Xuan S., Jiang M., Tian L., Tian J, & Chang Q. (2020). Expression of H2S in Gestational Diabetes Mellitus and Correlation Analysis with Inflammatory Markers IL-6 and TNF-α. Journal of Diabetes Research, 2020, 3085840.

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Measuring Lipid Peroxidation via MDA

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Malondialdehyde (MDA) concentration as an index of lipid peroxidation level, is measured using the thiobarbituric acid reaction. At first, 1 mL of diluted semen sample (250 × 10⁶) was mixed with 1 mL of cold 20% (w/v) trichloroacetic acid (TCA) to precipitate proteins. The precipitate was plated by centrifuging (960 × g) for 15 min, and 1 mL of the supernatant was incubated with 1 mL of 0.67% (w/v) thiobarbituric acid (TBA) in a boiling water-bath at 95°C for 10 min. After cooling, the absorbance was determined using a spectrophotometer (Shimadzu/UV-2100, Japan) at 532 nm; MDA concentrations is reported as nmol/mL (Fujihara and Koga, 1984 ).

Rezaie F.S., Hezavehei M., Sharafi M, & Shahverdi A. (2021). Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol. Poultry Science, 100(9), 101290.

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Genomic DNA Extraction from Plant Leaves

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DNA material was extracted from 1.0 g of fresh leaf material from five plants of each accession, using modified procedure according to Murray and Thompson [37 (link)]. The quantity and quality of total genomic DNA were determined by agarose gel electrophoresis and spectrophotometer UV-2100 (Shimadzu, Japan) absorbance at 230, 260, and 280 nm. Only DNA samples with the OD 260/OD 280 > 1.8 and OD 260/OD 230 ≥ 2.0 were diluted in sterile redistilled water and stored at −20 °C until use.

Cichorz S., Gośka M, & Litwiniec A. (2014). Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers. Molecular Biotechnology, 56(10), 911-924.

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Spectrophotometric Analysis System Design

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To create suitable reflective absorbance spectrophotometric equipment for analysis, a black acrylic chamber chose to disrupt reflective light from the white background as dimensions of 6.0 × 22 × 6.5 cm (width, length, and height) was designed as Model 3D consisting of LED strips (white light, 2.4-Watt constant, World semi) for a light controller. The instrument was built as a closed system with constant light intensity, in which the background of the instrument's interior, opposite to LED strips and a smartphone camera, as white to reduce light absorption. Also, the light intensity was focused through the chamber's circular cavity, as indicated in Fig. 1. Color grab (Loomatix Ver. 3.6.1) was applied as smartphone applications to detect the light intensity and to display RGB data, which indicates a value in the range of 0 -255 in each channel. A cuvette (Quartz cell, series No 06-907) with a capacity of 4.0 mL from Coax Group Corporation Ltd. was used in the experiment. A UV-Visible spectrophotometer (Shimadzu, UV2100) was used in the experiment.

Khachornsakkul K, & Dungchai W. (2021). A Portable Reflective Absorbance Spectrophotometric Smartphone Device for the Rapid and Highly Accurate Determination of Amlodipine in Pharmaceutical Formulation and Human Urine Samples. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 37(7).

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