Total protein extracts were generated using RIPA Lysis Buffer System (sc24948, SantaCruz Biotechnology) supplemented with Protease Inhibitor Cocktail set III (539134) and Phosphatase Inhibitor Cocktail Set II (524625) from Calbiochem. Protein was quantified using a BCA Protein Assay kit (23,225, ThermoFisher). Protein extracts (10 μg per sample) were loaded onto SDS-PAGE gels and electrophoretically transferred to PVDF membranes. The following primary antibodies were used: CD81 (sc-166,028, SantaCruz Biotechnology), TSG101 (ab83, Abcam) and b-actin (JLA20, Calbiochem). Goat anti-rabbit and anti-mouse HRP secondary antibodies were from Pierce ThermoScientific (31460) and Calbiochem (JA1200), respectively. Immunodetection of proteins was performed using Amersham ECL Western Blotting Detection Reagent (GE Healthcare).
Cd81 sc 166 028
Manufactured by Santa Cruz Biotechnology
Sourced in United States
CD81 (sc-166,028) is a mouse monoclonal antibody that recognizes the CD81 protein. CD81 is a member of the tetraspanin family of proteins, which are involved in various cellular processes, including cell adhesion, migration, and signaling.
Automatically generated - may contain errors
2 protocols using cd81 sc 166 028
1
Protein Extraction and Western Blot Analysis
2
EV Protein Characterization by Western Blot
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EV proteins (20 µg/lane) were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, transferred onto polyvinylidene fluoride membranes, probed with each primary antibody, and incubated with a horseradish peroxidase-conjugated secondary antibody. The following primary antibodies were used: CD63 (D4I1X, 1:500) and CD9 (D801A, 1:500) from Cell Signaling Technology (Danvers, MA, USA), CD81 (sc166028, 1:500) from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA), and cytochrome c (1:1,000) from BioLegend (San Diego, CA, USA).
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