Bx795 tlrl bx7

The small interfering RNA (siRNA) targeting mouse MDA-5 (sc-61011), RIG-I (sc-51481), IPS-1 (sc-75756), and control siRNA (sc-37007) targeting a scrambled sequence were purchased from Santa Cruz Biotechnology. BAY11-7082 (tlrl-b82) and BX795 (tlrl-bx7) were purchased from InvivoGen (San Diego, CA, USA). In Situ Cell Death Detection Kit (POD 11684817910) was purchased from Roche Diagnostics GmbH (Mannheim, Germany). The antibodies used in this study are listed in Table 1.

Cyclic [G(2′,5′)pA(3′,5′)p] (cGAMP) was obtained from BioLog. The dsDNA (HSV-1 60mer) was synthesize and annealed by DNA technology; sense strand 5′- CCA TCA GA AAG AG GTT TAA TA TTT TTG TGA GAC CAT CGA AGA GAG AAA GAG ATA AAA CTT; antisense 5′- AAG TTT TAT CTC TTT CTC TCT TCG ATG GTC TCA CAA AAA TAT TAA ACC TCT TTC TGA TGG. For more information see6 (link). The ssDNA1 sequences was 5′-GTC TCT CTG GTT AGA CCA GAT CTG AGC CTG GGA GCT CTC TGG CTA ACT AGG GAA CCC ACT GCT TAA GCC TCA ATA AAG CTT GCC TTG AGT GCT TCA AGT AGT G-3′. For more information see5 (link). Herring testis DNA was from Sigma Aldrich (D6898); BX795 (tlrl-bx7) and poly I:C (tlrl-pic) were both acquired from InvivoGen.

Anti-IFI16 (SAB1408587), anti-DAI (PRS4401), and anti-cGAS (SAB3500110) antibodies were purchased from Sigma (St. Louis, MS, USA). Anti-phospho-IRF3 (number 4947) and anti-ISG15 (number 2743) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin (sc-81178), anti-IRF3 (sc-9082), and anti-Mx1 (sc-50509) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-TLR3 (ab137722), anti-TLR7 (ab45371), anti-TLR8 (ab24185), anti-TLR9 (ab12121), anti-MDA5 (ab69983), anti-RIG-I (ab45428), anti-OAS1 (ab86343), and anti-Pol III (ab22236) antibodies were purchased from Abcam (Cambridge, UK). Poly(I:C)/Lyovec™ (tlrl-piclv), HSV60/Lyovec (tlrl-hsv60c), and BX795 (tlrl-bx7) were purchased from InvivoGen (San Diego, CA, USA). Small interfering RNA (siRNA) targeting RIG-I (sc-61480), TLR3 (sc036685), and IFI16 (sc-166504) and control siRNA (sc-37007) targeting a scrambled sequence were purchased from Santa Cruz Biotechnology.