Fluorouracil, selumetinib, BIO and NAC were purchased from Selleck Chemicals (Houston, TX, USA) and dissolved in DMSO or diluted water. DCF-DA was purchased from the Life Technology (Invitrogen, Carlsbad, California, USA) and dissolved in DMSO. Melatonin was from Sigma Aldrich (Sigma-Aldrich, St. Louis, USA). Antibodies include PARP, pAkt, Akt, pErk, Erk, GSK3β, pGSK3β, MEK, pMEK, β-Actin and GAPDH (Cell Signaling Technology, Beverly, MA, USA) as well as Ki-67 (Abcam, Cambridge, Massachusetts, USA).
Fluorouracil
Manufactured by Selleck Chemicals
Sourced in United States, China
Fluorouracil is a chemical compound used as a laboratory reagent. It functions as a pyrimidine analog, which is a class of organic compounds that mimic the structure of pyrimidines, a common chemical building block found in nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Oxaliplatin, by Selleck Chemicals (2 mentions)
Irinotecan, by Selleck Chemicals (2 mentions)
P erk, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Melatonin, by Merck Group (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
Selumetinib, by Selleck Chemicals (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
N acetyl cysteine (nac), by Selleck Chemicals (1 mentions)
P mek, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Cisplatin, by Selleck Chemicals (1 mentions)
Genistein, by Selleck Chemicals (1 mentions)
Gemcitabine, by Selleck Chemicals (1 mentions)
Leucovorin, by Selleck Chemicals (1 mentions)
4 protocols using fluorouracil
1
Cell Signaling Pathway Analysis
2
Cell Viability Assay of Chemotherapeutics
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Cells were seeded onto 96-well plates at a density of 3 × 103 cells/well and incubated with irinotecan, fluorouracil and oxaliplatin (Selleck, Houston, TX, USA) for 72 h. Then, the cells were stained with 20 μl of sterile MTS (3-(4, 5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl)-2H-tetrazolium) dye (Promega, Madison, WI, USA) for 2 h at 37 °C. The absorbance was measured at a wavelength of 490 nm. All experiments were conducted in triplicate.
3
Synergistic Anticancer Combination
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Genistein, cisplatin, and fluorouracil were purchased from Selleck Chemical (Shanghai, China), and stock solutions were prepared in dimethyl sulfoxide (Sigma-Aldrich, St Louis, MO, USA) at a concentration of 50, 10, and 10 mM, respectively. Antibody for KIF20A was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Actin antibody was obtained from TransBionovo (Beijing, China).
4
Evaluating FOLFIRINOX and Gemcitabine on PDAC Cells
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Cell viability was measured using MTT assay. PDAC cells were seeded in 96 well plates at a density of 4000 cells per well. To prepare FOLFIRINOX treatment, all the drugs were dissolved in Milli‐Q water and mixed, and the final concentration of 3 mg/mL fluorouracil (S1209, Selleck, China), 0.25 mg/mL oxaliplatin (S1224, Selleck, China), 2 mg/mL irinotecan (S1198, Selleck, China) and 2 mg/mL leucovorin (S1236, Selleck, China) is regarded as 100% FOLFIRINOX. After 24 hour, different concentrations of Gemcitabine (S1714, Selleck, China) or FOLFIRINOX were added to the medium to treat the cells. 72 hour later, the supernatant of each well was discarded and MTT agent (500 ug/mL) dissolved in serum‐free DMEM medium was added to each well. The supernatant was then discarded again after 2 hour. Finally, DMSO was added to dissolve the formazan, and the absorbance at 570 nm was measured by Multiscan Spectrum (SpectraMax M5).
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