Splenocytes were seeded at a density of 2.5*106 cells/mL on 96-well plates, and stimulated with or without 50 μg/mL endotoxin-free OVA, and incubated at 37°C in humidified air with 5% CO2 for 60 hours. Cells were incubated with 1% bovine serum albumin (BSA) in PBS overnight at 4°C, and blocked with TruStain FcX anti-CD16/CD32 (Biolegend, San Diego, CA) at a concentration of 1 μg/106 cells for 1 hour on ice. Alexa-Fluor 488-conjugated anti-CD44 and Alexa-Fluor 647-conjugated anti-CD62L (Biolegend, San Diego, CA) were added to each well at a final concentration of 1 μg/106 cells and 0.25 μg/106 cells, respectively, and incubated on ice for 1 hour. Cells were collected by centrifugation, resuspended in PBS, and analyzed by flow cytometry.
Alexa fluor 488 conjugated anti cd44
Manufactured by BioLegend
Alexa-Fluor 488-conjugated anti-CD44 is a fluorescently labeled antibody that can be used to detect and quantify CD44 expression on cells. CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The Alexa-Fluor 488 dye provides a green fluorescent signal that can be detected using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 488 conjugated anti cd44
1
OVA-Induced Splenocyte Phenotyping
2
Splenocyte Activation and Phenotyping
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Splenocytes were seeded at a density of 2.5 × 106 cells/ml on 96‐well plates, and stimulated with or without 50 μg/ml endotoxin‐free OVA, and incubated at 37°C in humidified air with 5% CO2 for 60 hr. Cells were incubated with 1% BSA in PBS overnight at 4°C, and blocked with TruStain FcX anti‐CD16/CD32 (Biolegend, San Diego, CA) at a concentration of 1 μg/106 cells for 1 hr on ice. Alexa‐Fluor 488‐conjugated anti‐CD44 and Alexa‐Fluor 647‐conjugated anti‐CD62L (Biolegend, San Diego, CA) were added to each well at a final concentration of 1 μg/106 cells and 0.25 μg/106 cells, respectively, and incubated on ice for 1 hr. Cells were collected by centrifugation, resuspended in PBS, and analyzed by flow cytometry.
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