Discover xt

Manufactured by Roche

Sourced in United States

The Roche Discover XT is a versatile laboratory instrument designed for high-throughput sample analysis. It is a core component of many research and diagnostic workflows, providing reliable and consistent performance across a wide range of applications.

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Lab products found in correlation

Dab map kit, by Roche (1 mentions)

Close spelling variants of this product

Ventana autostainer model Discover XT (9 citations) Ventana autostainer Discover XT (2 citations)

5 protocols using discover xt

Immunohistochemical Staining of Tumor Samples

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Immunohistochemical staining of tumor xenograft sections and spheroid sections was done on a Ventana autostainer model Discover XT (Ventana Medical System, Tuscan, Arizona) with an enzyme labeled biotin streptavidin system and solvent resistant DAB Map kit using corresponding primary antibody. For each biomarker, representative cores (clearly positive, clearly negative and mixed positive/negative) were manually identified by Dr. Ladan Fazli MD, Pathologist.

Nouri M., Caradec J., Lubik A.A., Li N., Hollier B.G., Takhar M., Altimirano-Dimas M., Chen M., Roshan-Moniri M., Butler M., Lehman M., Bishop J., Truong S., Huang S.C., Cochrane D., Cox M., Collins C., Gleave M., Erho N., Alshalafa M., Davicioni E., Nelson C., Gregory-Evans S., Karnes R.J., Jenkins R.B., Klein E.A, & Buttyan R. (2017). Therapy-induced developmental reprogramming of prostate cancer cells and acquired therapy resistance. Oncotarget, 8(12), 18949-18967.

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Prostate Cancer Tissue Microarray Analysis

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PCa tissue specimens were obtained from Vancouver Prostate Centre Tissue Bank. The H&E slides were reviewed to mark the desired areas and the corresponding paraffin blocks. Three tissue microarrays were manually constructed (Beecher Instruments, Sun Prairie, WI, USA) by punching duplicate cores of 1 mm for each sample. All specimens were from radical prostatectomy except 20 CRPC samples, which were obtained from transurethral resection of the prostate. Immunohistochemical staining was conducted by Ventana autostainer model Discover XT (Ventana Medical System, Tuscan, AZ, USA) with enzyme-labeled biotin streptavidin system and solvent-resistant DAB Map kit using 1:50 dilution of AR (N-20) and 1:10 dilution of Lyn (H-6), and Src antibodies. Specimens were graded from 0 to +3 intensity representing no staining to heavy staining by visual scoring. Automated quantitative image analysis was conducted using pro-plusimage software.

Zardan A., Nip K.M., Thaper D., Toren P., Vahid S., Beraldi E., Fazli L., Lamoureux F., Gust K.M., Cox M.E., Bishop J.L, & Zoubeidi A. (2014). Lyn tyrosine kinase regulates androgen receptor expression and activity in castrate-resistant prostate cancer. Oncogenesis, 3(8), e115-.

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Immunohistochemical Staining of DEK Protein

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Immunohistochemical staining with monoclonal mouse antibody against DEK (BD Biosciences, San Jose, CA, USA) was conducted using a Ventana autostainer (model Discover XT; Ventana Medical System, Tucson, AZ) with an enzyme-labelled biotin-streptavidin system and a solvent-resistant DAB Map kit (Ventana). All sections used for immunohistochemistry were counterstained with 5% (w/v) Harris hematoxylin.

Lin D., Dong X., Wang K., Wyatt A.W., Crea F., Xue H., Wang Y., Wu R., Bell R.H., Haegert A., Brahmbhatt S., Hurtado-Coll A., Gout P.W., Fazli L., Gleave M.E., Collins C.C, & Wang Y. (2014). Identification of DEK as a potential therapeutic target for neuroendocrine prostate cancer. Oncotarget, 6(3), 1806-1820.

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Decalcification and IHC Staining Protocol

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Before immunohistochemical staining decalcification was performed. Tissues were left in 0.5 molar EDTA on the shaker to facilitate fluid infiltration for a total of 7 days.. EDTA solution was refreshed every 2–3 days. Once decalcification was complete, tissue was washed with distilled water then store in 70 % ethanol prior to tissue processing. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 5 μm tumor sections using the appropriate primary antibody (haematoxylin and eosin)) and the Ventana autostainer Discover XT (Ventana Medical Systems).

Frees S., Breuksch I., Haber T., Bauer H.K., Chavez-Munoz C., Raven P., Moskalev I., D´Costa N., Tan Z., Daugaard M., Thüroff J.W., Haferkamp A., Prawitt D., So A, & Brenner W. (2018). Calcium-sensing receptor (CaSR) promotes development of bone metastasis in renal cell carcinoma. Oncotarget, 9(21), 15766-15779.

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Tumor Immunohistochemistry Protocol

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Immunohistochemical stains were performed on formalin-fixed and paraffin-embedded 3μm sections of tumor samples using adequate primary antibody, and the Ventana autostainer Discover XT (Ventana Medical System) with enzyme labeled biotin streptavidin system and solvent resistant 3,3′-diaminobenyidine Map kit. All comparisons of staining intensities were made at 200× magnifications.

Lamoureux F., Baud'huin M., Ory B., Guiho R., Zoubeidi A., Gleave M., Heymann D, & Rédini F. (2014). Clusterin inhibition using OGX-011 synergistically enhances zoledronic acid activity in osteosarcoma. Oncotarget, 5(17), 7805-7819.

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