The colon cancer cell lines (HCT116, COLO320, T24, HT29, SW480, SW948 and SW1417) used in our study were purchased from American Type Culture Collection (ATCC). The colon cancer cell line COLO678 was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). Human HEK293T was purchased from ATCC. The culture medium for SW480 and HEK293T was RPMI-1640 medium (Invitrogen, Carlsbad, California, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, Utah, USA), 100 U/mL Penicillium and 100 μg/mL Streptomycin. The culture medium for HCT116, COLO320, T24, HT29, SW480, SW948 and SW1417 were DMEM: F12 (1:1) medium (Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, Utah, USA), 100 U/mL Penicillium and 100 μg/mL Streptomycin. Cells were maintained at 37 °C in a humidified atmosphere with 5% CO2. Replaced the culture medium 2–3 times every week.
Sw1417
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SW1417 is a laboratory centrifuge designed for general purpose applications. It features a fixed-angle rotor that can accommodate multiple sample tubes or microplates. The centrifuge's speed and run time are adjustable to meet the requirements of various sample preparation and processing workflows.
Automatically generated - may contain errors
Lab products found in correlation
Colo678, by Leibniz Institute DSMZ (4 mentions)
Sw480, by Thermo Fisher Scientific (3 mentions)
Colo320, by Thermo Fisher Scientific (2 mentions)
Sw948, by Thermo Fisher Scientific (2 mentions)
Dmem f12 1 1 medium, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Sw480, by American Type Culture Collection (2 mentions)
Sw1417, by American Type Culture Collection (2 mentions)
Sw948, by American Type Culture Collection (2 mentions)
Colo320, by American Type Culture Collection (2 mentions)
Human hek293t, by American Type Culture Collection (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
2 protocols using sw1417
1
Culturing Colon Cancer Cell Lines
2
Culturing Colorectal Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Colon cancer cell lines COLO320, SW480, SW1417, SW948, T84, HT29 and human HEK293T cell line were obtained from American Type Culture Collection (ATCC). Colon cancer cell line COLO678 and CL11 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). SW480, COLO678 and HEK293T cells were cultured with RPMI-1640 medium (Invitrogen, USA). COLO320, SW1417, SW948, T84, HT29 and CL11 cells were cultured with DMEM: F12 (1:1) medium (Thermo Fisher Scientific, USA). All cells were supplemented with 10% fetal bovine serum (Hyclone, USA) and 1% PenStrep (100 U/mL Penicillium and 100 μg/mL Streptomycin) in a humidified incubator containing 5% CO2 at 37°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!