Fluorescein isothiocyanate (FITC)–, phycoerythrin (PE)- Allophycocyanin (APC)-, and Alexa-fluor- directly conjugated anti-human antibodies were used to stain the various cells tested: CD14 (21270146; ImmunoTools, Friesoythe, Germany), CD73 (21270734; ImmunoTools), CD8 (21270083; ImmunoTools), CD274 (12-5982-82; Invitrogen, Illkirch, France), CD279 (FAB7115G; R&D Systems, Biotechne, Abingdon, UK), and Granzyme B (IC2906G; R&D Systems). Analyses were performed using a LSR II 3 lasers cytofluorometer and Diva software (BD Biosciences, Le-Pont-de-Claix, France). For Granyme B intracellular staining, cells were permeabilized using the BD Cytofix/Cytoperm permeabilizing kit (AB_2869008; Beckton Dickinson, Le-Pont-de-Claix, France), and cells were analyzed 6 h after interaction with adipocytes.
Lsr 2 3 lasers cytofluorometer
Manufactured by BD
Sourced in France
The LSR II 3 lasers cytofluorometer is a flow cytometry instrument produced by BD. It is equipped with three lasers and is designed to analyze and sort cells or particles in a liquid suspension.
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2 protocols using lsr 2 3 lasers cytofluorometer
1
Multicolor Flow Cytometry for Immune Cell Profiling
2
Multicolor flow cytometry analysis
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FITC, PE, or APC conjugated mouse anti-human CD73, CD90, CD105, CD3, CD41 CD62P, human leukocyte antigen (HLA)-DR, intercellular adhesion molecule 1 (ICAM-1), CD8 (all from Immunotools) were used to label the various cells tested. Analyses were performed using the “LSR II 3 lasers” cytofluorometer and the Diva software (both were from BD Biosciences).
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