Cells were seeded in 24-well plates and allowed to adhere for 24 h. After replacing with serum-free media, the cells were maintained overnight. For cytotoxicity test, RM9 cells were incubated with PLGA-ICG-R848 with different concentrations of ICG for 4 h and irradiated without or with 808 nm laser (1W/cm2) for 10 min. After 24 h, the cell viability was detected using cell proliferation kit (Roche, Germany). For cell proliferation assay, RM9, PC-3, LNCaP and DU-145 cells were firstly treated for 4 h with PBS, PLGA, PLGA-R848, PLGA-ICG and PLGA-ICG-R848, containing the same concentration of ICG or R848 and next irradiated without or with 808nm laser (1W/cm2) for 10 min. After 24 h, the cell viability was detected using cell proliferation kit (Roche, Germany).
Cell proliferation kit
Manufactured by Roche
Sourced in United States, Germany, Italy, Switzerland
The Cell Proliferation Kit is a laboratory tool designed to measure and analyze cell growth and proliferation. It provides a standardized method for quantifying the number of viable cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Methyl thiazolyl tetrazolium (mtt), by Roche (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Tov 21g, by American Type Culture Collection (2 mentions)
Flag and β actin, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Lipofectamine plus, by Thermo Fisher Scientific (2 mentions)
T stat3, by Cell Signaling Technology (2 mentions)
P stat3, by Cell Signaling Technology (2 mentions)
Oligofectamine, by Thermo Fisher Scientific (2 mentions)
Annexin 5 pi kit, by BD (2 mentions)
Myc tag, by Roche (2 mentions)
Yac 1, by American Type Culture Collection (2 mentions)
Microtiter plate reader, by Promega (1 mentions)
Epilife medium, by Thermo Fisher Scientific (1 mentions)
Asys uvm 340 mikrowin 2000, by Harvard Bioscience (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Mco 18ac, by Sanyo (1 mentions)
Prism software, by GraphPad (1 mentions)
Wst 1, by Roche (1 mentions)
2 mercaptoethanol me, by Merck Group (1 mentions)
74 protocols using cell proliferation kit
1
Cytotoxicity and Proliferation Assay of PLGA-based Photothermal Therapy
2
Colorimetric Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For assay of cell viability, cells were seeded into 24 well-plate at 10000 cells per well and subjected to a Cell Proliferation Kit (MTT, Roche, Indianapolis, IN, USA), according to the instruction of the manufacturer. The MTT assay is a colorimetric assay for assessing viable cell number, taking advantage that NADPH-dependent cellular oxidoreductase enzymes in viable cells reduce the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble formazan in purple readily being quantified by absorbance value (OD) at 570 nm. Experiments were performed 5 times.
3
Prostate Cancer Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of the two compounds in the proliferation of human prostate cancer cell line was determined as previously described by using the XTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate) cell proliferation kit (Roche Molecular Biochemicals, Mannheim, Germany) as previously described [11 (link),12 (link)]. Cells (5.0 × 103 in 100 μL) were incubated in RPMI-1640 culture medium containing 10% heat-inactivated FBS, in the absence and in the presence of the indicated compounds at a concentration range of 10−3 to 10−9 M, in 96-well flat-bottomed microtiter plates, and following 72 h of incubation at 37 °C in a humidified atmosphere of air/CO2 (19/1), the XTT assay was performed. Measurements were done in triplicate, and each experiment was repeated three times. The IC50 (50% inhibitory concentration) value, defined as the drug concentration required to cause 50% inhibition in the cellular proliferation with respect to the untreated controls, was determined for each compound.
4
Evaluating Organoid Viability via MTT Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organoid viability was evaluated using a cell proliferation kit (Roche, Germany). In brief, after culture medium removal, 10 μl MTT-labeling reagent was added to the organoid culture for 1 h. Viable organoids, which could reduce the MTT reagent to formazan, were imaged using a light microscope. After adding DMSO to solubilize the formazan crystals, the absorbance of the colored solution was measured using a microplate reader at 575 nm and a reference at 650 nm. Cell viability was calculated as the percentage of viable cells relative to the non-treated group.
5
MTT Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assayed based on the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by using a cell proliferation kit (Roche Korea, Seoul, Korea). HaCaT cells in 100 μL culture medium were seeded into a 96‑well plate, and CS extract was added to the wells at concentrations ranging between 10 and 50 μg/mL. Following incubation for 48 h at 37°C, 10 μL MTT solution was added, followed by incubation for 4 h. Solubilization solution (100 μL) was added to the wells and following 24 h incubation, absorbance was measured at 550 nm by using an ELx808 enzyme‑linked immunosorbent assay (ELISA) reader (Bio‑Tek Instruments, Winooski, VT, USA).
6
Cytotoxicity of cis- and trans-TMS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytotoxicity assay used the Cell Proliferation Kit (Roche Applied Science). On a 96 wells plate, 104 cells/well were seeded and incubated in an atmosphere of 5% CO2 at 37 °C for 24 h. After this period, the cells were exposed to different concentrations of cis-TMS and trans-TMS (7.8 to 1000 µM), 0.5 µM of doxorubicin, or 0.1% of DMSO. Doxorubicin and DMSO were positive and vehicle control, respectively. The cells were incubated for an additional 24 h and, the plate was washed with phosphate buffer saline (PBS 1x). The plate was then incubated with DMEM without phenol red plus the XTT/electron solution for 4 h. Total absorbance was measured at 492 and 690 nm. The number of viable cells was directly proportional to the absorbance and the percentage was compared with the negative control.
7
MTT Assay for Viable Cell Number
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For assay of viable cell number, 5×10 3 cell per well were seeded into 96 well-plate and subjected to a Cell Proliferation Kit (MTT, Roche, Indianapolis, IN, USA), according to the instruction of the manufacturer. The MTT assay is a colorimetric assay for assessing viable cell number, taking advantage that NADPH-dependent cellular oxidoreductase enzymes in viable cells reduce the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble formazan in purple readily being quantified by absorbance value (OD) at 570 nm in a microtiter plate reader (Promega, Fitchburg, WI, USA). Experiments were performed 5 times.
8
Cell Proliferation Assay with NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WST-1 assay was used to investigate the cell proliferation. Cells were seeded on 96-well plate at a density is 10,000 cells/well and incubated overnight. The cell viability was estimated after cells exposure to different concentration of NPs for 24 h by WST-1 assay (cell proliferation kit, Roche). The absorption is measured by a spectrophotometer (ELISA reader) at a wavelength of 450 nm.
9
Cell Viability Assay for N2a Cells
10
Cell Proliferation Assay with 6-HAP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B16F10, Pam212, L5178, and YAC-1 cell lines were obtained from American Type Culture Collection (ATCC). Pam212, L5178, and YAC-1 cell lines were maintained directly from the authenticated vial from ATCC in RPMI 1640 supplemented with sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), penicillin (100 unit/ml), streptomycin (100 μg/ml), and 10% heat-inactivated fetal bovine serum (FBS) or horse serum at 37°C under atmosphere of 5% (v/v) CO2 in air. B16F10 cell line was maintained directly from the authenticated vial from ATCC in Dulbecco’s modified Eagle’s medium supplemented with penicillin (100 unit/ml), streptomycin (100 μg/ml), and 10% heat-inactivated FBS. NHEKs were obtained from Invitrogen (Life Technologies) and maintained in EpiLife medium (Life Technologies) supplemented with 60 μM calcium, epidermal growth factors, penicillin, and streptomycin. After a 4-hour (tumor cell lines) or 24-hour (NHEK) incubation with 6-HAP, proliferative activity of cells was colorimetrically determined by monitoring BrdU incorporation with Cell Proliferation kit according to the manual (Roche).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!