Colorimetric WST‐1 assay (Roche) was used to determine cell viability. The absorbance was measured by a spectrophotometer (SpectraMax Plus from Molecular Devices) at 450 nm against a reference at 690 nm. The optical density values relative to the control cells in the assay represent the percentage of viable cells. To detect cell apoptosis, the TUNEL assay was performed using the ApoAlert™ DNA Fragmentation Assay Kit (Clontech) to detect the presence of DNA fragmentation in frozen tissue sections. The fixed sections were washed twice with phosphate‐buffered saline (PBS) before incubating in the permeabilization solution (0.2% Triton X‐100 in PBS) on ice for 10 min. The sections were washed twice in PBS and then incubated in TUNEL reaction mixture at 37°C in the dark in a humidified atmosphere for 1 h. The stained sections were washed once again with PBS before mounting with 4′,6‐diamidino‐2‐phenylindole mounting medium (VECTASHIELD) for fluorescence microscopy analysis.
Wst 1 colorimetric assay
Manufactured by Roche
Sourced in Germany
The WST-1 colorimetric assay is a cell proliferation and viability reagent. It is used to measure the metabolic activity of cells in culture. The assay utilizes the tetrazolium salt WST-1 (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium), which is reduced by cellular enzymes to form a colored formazan dye. The intensity of the color produced is directly proportional to the number of viable cells, and can be quantified by measuring the absorbance of the solution.
Automatically generated - may contain errors
Lab products found in correlation
Apoalert dna fragmentation assay kit, by Takara Bio (1 mentions)
Spectramax plus, by Molecular Devices (1 mentions)
Brdu elisa kit, by Roche (1 mentions)
Varioscan lux device, by Thermo Fisher Scientific (1 mentions)
Caspase glo 3 7 assay kit, by Promega (1 mentions)
Imatinib mesylate, by Novartis (1 mentions)
Microplate autoreader, by Bio-Rad (1 mentions)
Versamax elisa microplate reader, by Molecular Devices (1 mentions)
Prism 6, by GraphPad (1 mentions)
Spectramax 190 microplate reader, by Molecular Devices (1 mentions)
Trypan blue stain, by Thermo Fisher Scientific (1 mentions)
Softmax pro 5, by Molecular Devices (1 mentions)
Versamax microplate reader, by Molecular Devices (1 mentions)
Apoptosis detection kit, by BD (1 mentions)
Facsverse, by BD (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Superfect, by Qiagen (1 mentions)
Gh3 cells, by American Type Culture Collection (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
33 protocols using wst 1 colorimetric assay
1
Quantifying Cell Viability and Apoptosis
2
Evaluating Cellular Viability via WST-1 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For evaluation of cellular viability 2 × 104 cells per well were seeded into a 96-well plate. Following incubation with 3 μM THC or vehicle for 6 h (Figure 1B ), viability was tested by the colorimetric WST-1 assay (Roche Applied Science, Mannheim, Germany). This assay is based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1.3-benzene disulfonate) to formazan by mitochondrial dehydrogenase activity.
3
Mitochondrial and DNA Synthesis Assays
4
Colorimetric Viability Assay of hBMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Colorimetric WST-1 assay (WST Roche Diagnostic GmbH, Mannheim, Germany) was used to evaluate viable cell counts of hBMSCs after 24 h, 3 days and 7 days. Briefly, the medium from the cell/scaffold constructs was replaced with WST-1 at the ratio of 1:10 with the respective medium used. Samples were incubated in the dark for 4 h at 37°C in 5% CO2. A micro-plate reader (BMG LABTECH, GmbH, Germany) at 450 nm was used to measure the absorbance.
5
Linalool Effect on HepG2 Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2 cells seeded in 96-well plates at a density of 5 × 10 3 cells per well were incubated in medium supplemented with DMSO 0.1 % or linalool (0-2.5 mM). Cell viability was determined using a colorimetric WST-1 assay (Roche, Vilvoorde, Belgium) according to the manufacturer´s instructions.
6
Cell Proliferation and Apoptosis Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary tumor cells or GH3 cells were plated in 96‐well plates (104cells/well). Cell proliferation/viability was measured using WST‐1 colorimetric assay (Roche) 48 h after treatment or 24 h, 48 h, 72 h after transfection according to the manufacturer’s recommendations.
Apoptosis assay was performed using Caspase‐Glo® 3/7 Assay kit (Promega) to assess the activity of caspase‐3/7 48 h after treatment or 24 h, 48 h, 72 h after transfection. Absorbance and luminescence was measured using Thermo Scientific Varioscan LUX device.
Apoptosis assay was performed using Caspase‐Glo® 3/7 Assay kit (Promega) to assess the activity of caspase‐3/7 48 h after treatment or 24 h, 48 h, 72 h after transfection. Absorbance and luminescence was measured using Thermo Scientific Varioscan LUX device.
7
Evaluating miR-125a-5p Impact on Imatinib Sensitivity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of miR-125a-5p overexpression or inhibition on imatinib response was evaluated using the WST-1 colorimetric assay (Roche Applied Science, Mannheim, Germany), as previously described (Caramuta et al, 2010 (link)). After 48 h of transfection, 30 000 cells per well were seeded in 96-well plates and were left either untreated (as mock control) or treated with three different concentrations of imatinib (0.05 μM , 0.1 μM and 0.5 μM for GIST882; 1 μM , 5 μM and 10 μM for GIST48). All experiments were conducted in five wells for each condition and replicated at least three times in independent experiments. The percentage of surviving cells was calculated by comparing the absorbance values of the samples after background subtraction and normalised to the respective cells without imatinib treatment. Imatinib mesylate was kindly provided by Novartis Pharma (Basel, Switzerland).
8
Measuring Cellular Proliferation and Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to measure cell proliferation and viability, Ca9-22 cells were seeded into 24-well plates (1.0 × 105 cells/well) and transfected with either Pre-miR-223 or Pre-miR-NC at the specified concentrations. Cell samples were harvested at 24, 48, 72, 96, 120, and 144 h after transfection. The number of cells was determined by manual hemocytometer counting, and viability was assessed using the trypan blue exclusion method. Alternatively, cells seeded in 96-well plates (1.0 × 104 cells/well) and transfected with the corresponding Pre-miRs. WST-1 colorimetric assay (Roche, Indianapolis, IN) were used for simultaneous measurement of cell proliferation and viability at various time points or with various quantities of Pre-miRNAs. Briefly, 10 μl of ready-to-use WST-1 solution was added directly to the cell culture in a 96-well plate. After incubation for 2 h, absorbance at 450 nm was measured using a microplate autoreader (BioRad, Hercules, CA) with a reference wavelength of 690 nm.
9
Cell Viability Assay in Testicular Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell growth was measured by WST-1 colorimetric assay (no. 11644807001; Roche Diagnostics, Indianapolis, IN, USA) in TCam-2 and 2102Ep cells 72 h after transfection. Cells were plated into a 96-well plate at a concentration of 5×103/well in 100 μl culture medium. At different time intervals (0, 24, 48 or 72 h after transfection), 10 μl of WST-1 reagent was added to each well and incubated for 3 h at 37°C. After incubation, absorbance values were detected at the wavelengths 450 nm (measurement) and 650 nm (reference) using the VERSAmax ELISA Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). Each experimental group was performed in six replicates for each time-point and all experiments were repeated three times independently.
10
Cytotoxicity Screening of Drug Candidates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were grown in 96-well trays for 24 h with increasing levels of drug. Metabolic activity was measured using WST-1 colorimetric assay (Roche) where dye formed from a tetrazolium compound and an electron coupling reagent directly correlates to the number of metabolically active cells in the culture. IC50 values were calculated (Prism 6, Graph Pad Software Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!