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Dmire2 epifluorescence dic microscope

Manufactured by Leica
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The DMIRE2 is an epifluorescence/DIC microscope manufactured by Leica. It is designed for imaging and analysis of fluorescently labeled samples. The microscope features a motorized focus drive and a high-intensity illumination system for fluorescence applications.

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DMIRE2 (204 citations) Epifluorescence microscope (146 citations) DMIRE2 microscope (135 citations) DMIRE2 epifluorescence microscope (7 citations) DIC microscope (2 citations)

2 protocols using dmire2 epifluorescence dic microscope

1

Quantifying ZnMP Uptake in L4 Worms

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To assess uptake of ZnMP, synchronized L1 worms were grown on NGM plates seeded with OP50 or RNAi bacteria until they reached L4 stage. Worms were washed off plates and rinsed three times in M9 buffer to remove bacteria. Worms were incubated in mCeHR2 medium with 10 μM ZnMP overnight, rinsed to remove excess ZnMP, and imaged as described previously10 (link). Briefly, worms were paralyzed with 10 mMlevamisole and imaged using a Leica DMIRE2 epifluorescence/DIC microscope. Images were obtained using a Retiga 1300 cooled mono 12-bit camera and quantified using SimplePCI software (Compix Inc).
Sinclair J., Pinter K., Samuel T., Beardsley S., Yuan X., Zhang J., Meng K., Yun S., Krause M, & Hamza I. (2017). Inter-organ signaling by HRG-7 promotes systemic heme homeostasis. Nature cell biology, 19(7), 799-807.
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2

Quantifying ZnMP Uptake in L4 Worms

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess uptake of ZnMP, synchronized L1 worms were grown on NGM plates seeded with OP50 or RNAi bacteria until they reached L4 stage. Worms were washed off plates and rinsed three times in M9 buffer to remove bacteria. Worms were incubated in mCeHR2 medium with 10 μM ZnMP overnight, rinsed to remove excess ZnMP, and imaged as described previously10 (link). Briefly, worms were paralyzed with 10 mMlevamisole and imaged using a Leica DMIRE2 epifluorescence/DIC microscope. Images were obtained using a Retiga 1300 cooled mono 12-bit camera and quantified using SimplePCI software (Compix Inc).
Sinclair J., Pinter K., Samuel T., Beardsley S., Yuan X., Zhang J., Meng K., Yun S., Krause M, & Hamza I. (2017). Inter-organ signaling by HRG-7 promotes systemic heme homeostasis. Nature cell biology, 19(7), 799-807.
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