To measure antigen-specific antibody levels in immune sera, inactivated A/Cal H1N1 or rgH5N1 virus (200 ng/well) were coated onto the ELISA plates before adding the diluted immune sera. Anti-mouse immunoglobulin (Ig) G, IgG1 and IgG2a (or IgG2c) HRP-labeled secondary antibodies were used to detect the antigen-specific Ig in the sera. To detect cytokines and chemokines in lung extracts and peritoneal exudates, interleukin(IL)-1β, IL-6, IL-10, interferon (IFN)-γ ready-set-go kits (eBioscience) and monocyte chemoattractant protein 1 (MCP-1, CCL2), regulated on activation, normal T cell expressed and secreted (RANTES, CCL5), KC (CXCL1), Interferon gamma-induced protein 10 (IP-10, CXCL10) chemokine kits (R&D systems) were used by following the manufacturer’s manual.
Interferon ifn γ ready set go kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Interferon (IFN)-γ ready-set-go kits are laboratory equipment designed to measure the levels of IFN-γ in a sample. These kits provide a standardized and reliable method for the quantitative detection of IFN-γ.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using interferon ifn γ ready set go kits
1
Quantifying Virus-Specific Antibodies and Immune Mediators
2
Quantification of Antigen-Specific Antibodies and Cytokines
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Immune sera were collected 2 weeks after immunization and antigen-specific antibody levels were determined by ELISA. Briefly, ELISA plates were coated with inactivated A/PR8 virus (200 ng/well) and blocked with 1% bovine serum albumin and 0.05% tween20 in PBS. Immune sera were serially diluted and added to the ELISA plates. After washing, horseradish peroxidase-labeled secondary antibody was incubated to detect antigen-specific IgG, IgG1, and IgG2a antibodies. Tetramethylbenzidine (TMB) was used as a substrate and OD measured at 450 nm by an ELISA reader (Bio-Rad).
To measure cytokine and chemokine levels in lung extracts and cell culture supernatants, IL-1β, IL-6, IL-10 IL-12p70, TNF-α, interferon (IFN)-γ ready-set-go kits (eBioscience, Sand Diego, CA, USA) and monocyte chemoattractant protein 1 (MCP-1), regulated on activation, normal T cell expressed and secreted (RANTES), keratinocyte-derived chemokine (KC), IFN gamma-induced protein 10 (IP-10) chemokine kits (R&D systems, Minneapolis, MN, USA) were used by following the manufacturer's manual.
To measure cytokine and chemokine levels in lung extracts and cell culture supernatants, IL-1β, IL-6, IL-10 IL-12p70, TNF-α, interferon (IFN)-γ ready-set-go kits (eBioscience, Sand Diego, CA, USA) and monocyte chemoattractant protein 1 (MCP-1), regulated on activation, normal T cell expressed and secreted (RANTES), keratinocyte-derived chemokine (KC), IFN gamma-induced protein 10 (IP-10) chemokine kits (R&D systems, Minneapolis, MN, USA) were used by following the manufacturer's manual.
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