Nestin antibody

Manufactured by R&D Systems

Sourced in United States

Nestin antibody is a protein used in immunohistochemistry and other research applications to detect the presence of the nestin protein. Nestin is an intermediate filament protein that is expressed in neural stem and progenitor cells. The antibody can be used to identify and study these cell types.

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Facscalibur, by BD (1 mentions)

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Nestin (28 citations)

2 protocols using nestin antibody

Measuring Stem Cell Markers by Flow Cytometry

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Cell suspensions were stained for human nestin (Nestin antibody, R&D Systems, Minneapolis, MN) and bCD (bCD antibody, BD Pharmingen, San Jose, CA) and analyzed by flow cytometry to measure percentages of Nestin-positive and bCD-positive cells.24 (link), 25

Tirughana R., Metz M.Z., Li Z., Hall C., Hsu D., Beltzer J., Annala A.J., Oganesyan D., Gutova M, & Aboody K.S. (2018). GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System. Molecular Therapy. Methods & Clinical Development, 10, 48-56.

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Isolation and Characterization of Glioma Stem Cells

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C6 glioma stem cells were cultured in serum-free medium for 14 days and then separated by trypsin in order to obtain stem cell spheres. Washed them by PBS and settled them by 4% paraformaldehyde. Their membrane were ruptured by 0.1% saponin, followed by cultured with Nestin antibody, and their appropriate isotype controls (R&D Systems, Minneapolis, MN, USA) for 30 minutes away from light. After that, the stem cells were washed by PBS three times and analysed with FACScan flow cytometry (Becton Dickinson FACSCalibur, Mountain View, CA, USA) [44 (link), 45 (link)].

Ying X., Wang Y., Xu H., Li X., Yan H., Tang H., Wen C, & Li Y. (2017). The construction of the multifunctional targeting ursolic acids liposomes and its apoptosis effects to C6 glioma stem cells. Oncotarget, 8(38), 64129-64142.

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