125i camp

6’-Guanidinonaltrindole (6’-GNTI), U50488, naltrindole, U0126, and λ-Carrageenan were purchased from Sigma-Aldrich (St. Louis, MO). [D-Pen2,D-Pen5]Enkephalin (DPDPE), and bradykinin (BK) were purchased from Bachem Americas, Inc. (Torrance, CA). ICI-199441 was purchased from Tocris (Minneapolis, MN). Prostaglandin E2 (PGE₂), 12-HETE, 15-HETE, baicalein and luteolin were purchased from Cayman Chemicals (Ann Arbor, MI). SCH772984 was purchased from Selleckchem (Houston, TX). ¹²⁵I-cAMP was purchased from PerkinElmer Life and Analytical Sciences (Waltham, MA). Nerve growth factor was from Harlan (Houston, TX). Collagenase was from Worthington (Lakewood, NJ). Hank’s balanced salt solution, feta l bovine serum, Dulbecco’s modified Eagles Medium (DMEM) were purchased from Invitrogen Corp. (Carlsbad, CA). All other drugs and chemical (reagent grade) were purchased from Sigma-Aldrich.

[¹²⁵I]cAMP, [³²P]Pi, and [³H]myo-inositol were obtained from PerkinElmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1 X 8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to PLC-β3 and RGS2, and Gα_i3 were from Santa Cruz biotechnology, Santa Cruz, CA; myristoylated PKI 14–22 aminde, Rp-cGMPS and Ht-31 were from Calbiochem, La Jolla, CA; RNAqueous^™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from QIAGEN Sciences, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript^™ II Reverse Transcriptase and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, CA; EcoR1 was from New England Bio Labs; All other chemicals were from Sigma, St. Louis, MO.
All animal procedures were conducted in accordance with the Institutional Animal Care and Use Committee of the Virginia Commonwealth University.