Rabbit anti human cleaved caspase 3 asp175 polyclonal antibody

Manufactured by Cell Signaling Technology

This rabbit polyclonal antibody recognizes the cleaved form of human caspase-3, which is a key effector of apoptosis. It specifically binds to the Asp175 cleavage site of the caspase-3 protein.

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Lab products found in correlation

Rabbit anti human ki 67 clone sp6 monoclonal antibody, by Thermo Fisher Scientific (2 mentions) Brightvision horseradish peroxidase conjugated goat anti rabbit polyclonal secondary antibody, by Immunologic (2 mentions) Rabbit anti human npm polyclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human p npm thr199 polyclonal antibody, by Cell Signaling Technology (1 mentions) Carfilzomib, by Adipogen (1 mentions) Mg132, by Peptide Institute (1 mentions) Goat anti rabbit igg horseradish peroxidase hrp linked antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human caspase 7 antibody, by Cell Signaling Technology (1 mentions) Dulbecco s modified eagle s medium, by Fujifilm (1 mentions) Zvad fmk, by Bachem (1 mentions) Mdl28170, by Merck Group (1 mentions)

3 protocols using rabbit anti human cleaved caspase 3 asp175 polyclonal antibody

Immunohistochemical Analysis of NPM, p-NPM, Ki-67, and Cleaved Caspase-3

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The following antibodies were used: rabbit anti-human NPM polyclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-human p-NPM (Thr199) polyclonal antibody (1:400, Cell Signaling Technology); rabbit anti-human Ki-67 (clone SP6) monoclonal antibody (1:1000, Thermo Scientific), rabbit anti-human cleaved caspase 3 (Asp175) polyclonal antibody (1:100, Cell Signaling Technology) and BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody (undiluted; 30 min; Immunologic). See Supplementary Materials and Methods for a detailed protocol.

Dolman M.E., Poon E., Ebus M.E., den Hartog I.J., van Noesel C.J., Jamin Y., Hallsworth A., Robinson S.P., Petrie K., Sparidans R.W., Kok R.J., Versteeg R., Caron H.N., Chesler L, & Molenaar J.J. (2015). Cyclin-dependent kinase inhibitor AT7519 as a potential drug for MYCN-dependent neuroblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(22), 5100-5109.

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Immunohistochemical Analysis of Proliferation and Apoptosis

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The following antibodies were used: rabbit anti-human Ki-67 (clone SP6) monoclonal antibody (1:1,000, Thermo Scientific), rabbit anti-human cleaved caspase 3 (Asp175) polyclonal antibody (1:100, Cell Signaling Technology) and BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody (undiluted; 30 min; Immunologic). See Supplementary Materials and Methods for a detailed protocol.

Bate-Eya L.T., den Hartog I.J., van der Ploeg I., Schild L., Koster J., Santo E.E., Westerhout E.M., Versteeg R., Caron H.N., Molenaar J.J, & Dolman M.E. (2016). High efficacy of the BCL-2 inhibitor ABT199 (venetoclax) in BCL-2 high-expressing neuroblastoma cell lines and xenografts and rational for combination with MCL-1 inhibition. Oncotarget, 7(19), 27946-27958.

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Proteasome Inhibitors Effectiveness Study

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The compounds used in this study were purchased as follows. BTZ was from FUJIFILM Wako Pure Chemical. MLN9708 (IXA citrate), delanzomib, and z-FA-FMK were from Cayman Chemical. MDL-28170 was from Merck. Proteasome inhibitors MG132, MG115, and PSI were from the PEPTIDE Institute. Carfilzomib was from Adipogen. z-VAD-FMK was from Bachem. These compounds were separately dissolved in DMSO (Nacalai tesque) at 10 mM and diluted with Dulbecco’s Modified Eagle’s Medium (FUJIFILM Wako Pure Chemical).
The antibodies used in this study were purchased as follows. Rabbit anti-human cleaved caspase-3 (Asp175) polyclonal antibody (#9661), rabbit anti-human caspase-7 antibody (#9492), rabbit anti-human cleaved PARP (Asp214) polyclonal antibody (#9541), goat anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (#7074), and horse anti-mouse IgG HRP-linked antibody (#7076) were from Cell Signaling Technology. Mouse anti-β-actin (C4) monoclonal antibody (sc-47778) was from Santa Cruz Biotechnology.

Itoh R., Kurihara Y., Yoshimura M, & Hiromatsu K. (2022). Bortezomib Eliminates Persistent Chlamydia trachomatis Infection through Rapid and Specific Host Cell Apoptosis. International Journal of Molecular Sciences, 23(13), 7434.

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