Rabbit polyclonal survivin

Manufactured by Novus Biologicals

Sourced in United States

Rabbit polyclonal survivin is a laboratory research tool used to detect and study the survivin protein, which is involved in regulating cell division and apoptosis. This antibody is produced by immunizing rabbits with a survivin antigen, resulting in a polyclonal population of antibodies that recognize multiple epitopes on the survivin protein.

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4 protocols using rabbit polyclonal survivin

Western Blot Analysis of Apoptotic Proteins

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Western blot analysis was performed as previously reported [26 (link)]. In brief, total cellular protein concentration was determined using the BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/DIABLO, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-activated caspase-3, mouse monoclonal anti-PARP (BD Pharmingen, USA), rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit anti-actin, an affinity isolated antigen specific antibody (Sigma, Saint Louis, MO), rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).
Blots suggesting regulation of loading controls actin or GAPDH by Ym155 and/or JL5 were then probed with spectrin, which is also a cytoskeletal protein.

Mondal A., Roberge J., Gilleran J., Peng Y., Jia D., Akel M., Patel Y., Zoltowski H., Doraiswamy A, & Langenfeld J. (2022). Bone morphogenetic protein inhibitors and mitochondria targeting agents synergistically induce apoptosis-inducing factor (AIF) caspase-independent cell death in lung cancer cells. Cell Communication and Signaling : CCS, 20, 99.

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Comprehensive Western Blot Analysis of Apoptosis Markers

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Western blot analysis was performed as previously reported [3] . In brief, total cellular protein concentration was determined using the BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/DIABLO, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit anti-actin, an a nity isolated antigen speci c antibody (Sigma, Saint Louis, MO), rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).
Blots suggesting regulation of loading controls actin or GAPDH by Ym155 and/or JL5 were then probed with the spectrin, which is also a cytoskeletal protein.

Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce Apoptosis-Inducing Factor (AIF) caspase independent cell death.

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Comprehensive Western Blot Analysis of Apoptosis Markers

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Western Blot Analysis of Cell Signaling

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Western blot analysis was performed as previously reported [3] . In brief, total cellular protein concentration was determined using BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/Diablo, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-pTAK1, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit antiactin, an a nity isolated antigen speci c antibody (Sigma, Saint Louis, MO), and rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).

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