The human melanoma cell line COLO829, human breast cancer cell line HCC38, and murine colon tumor cell line CT26 were purchased from ATCC (Manassas, VA, USA). Human CRC cell lines Caco2, DLD1, HCT116, LoVo, HT29, and RKO were gifts from Dr. Hirofumi Yamamoto (Osaka University Graduate School of Medicine, Japan). All human cell lines were authenticated by ATCC using DNA profiling. Cells were maintained in appropriate media as follows: DMEM (DLD1 and HCT116), RPMI (HT29, RKO, COLO829, CT26, and HCC38), and Hanks-F12K (LoVo) supplemented with 10% fetal bovine serum, 10,000 units penicillin, 10 mg/ml streptomycin, and 25 μg/ml amphotericin B. Culture media and fetal bovine serum were obtained from Life Technologies Japan (Tokyo, Japan). All cells were grown at 37°C in a humidified incubator with 5% CO2. To obtain FR-resistant cells, cells were cultured in the presence of high concentrations of FR (20 ng/ml for DLD1 and 10 ng/ml for all other cell lines).
Hcc38
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HCC38 is a compact and versatile laboratory instrument designed for DNA/RNA analysis. It features a high-intensity LED light source and a sensitive photodetector for accurate fluorescence measurements. The HCC38 is capable of handling a variety of sample formats, including microplates, cuvettes, and tubes.
Automatically generated - may contain errors
Lab products found in correlation
Hcc38, by American Type Culture Collection (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Mda mb 468, by Thermo Fisher Scientific (5 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Mda mb 231, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Mcf 7, by Thermo Fisher Scientific (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Mda mb 231, by American Type Culture Collection (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Mda mb 453, by Thermo Fisher Scientific (3 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Mda mb 468, by American Type Culture Collection (3 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Hepes, by Thermo Fisher Scientific (2 mentions)
1640 medium, by Thermo Fisher Scientific (2 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions)
Hcc1937, by Thermo Fisher Scientific (2 mentions)
Hcc1954, by Thermo Fisher Scientific (2 mentions)
Hcc70, by Thermo Fisher Scientific (2 mentions)
13 protocols using hcc38
1
Characterization of FR-resistant cancer cell lines
2
TNBC Cell Culture Protocols
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HCC38, MDA-MB-231 and MDA-MB-453 TNBC cells were purchased from the American Type Culture Collection (ATCC, LGC Promochem, Karnataka, India), authenticated by short tandem repeat profiling in 2021 (not shown). HCC38 cells were cultured in RPMI-1640 (LifeTechnologies, Carlsbad, CA, USA) supplemented with 10% (vol/vol) fetal bovine serum (FBS, LifeTechnologies), 1.5 g/L sodium bicarbonate (LifeTechnologies), 10 mmol/L Hepes (LifeTechnologies), 1 mmol/L sodium pyruvate (LifeTechnologies), 100 U/mL penicillin, and 100 µg/mL streptomycin (LifeTechnologies). MDA-MB-453 and MDA-MB-231 cells were cultured in DMEM-F12 (LifeTechnologies) supplemented with 10% FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin.
3
Cytotoxicity Evaluation of Plant Extracts
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HCC-38 (CRL-2314™, homosapien, mammary carcinoma epithelial cells, estrogen receptor negative), MDA-MB-361 (HTB-27™, homosapien, mammary gland/breast; derived from metastatic site: brain) and Vero (CCL-81™, normal kidney cells) cell lines were obtained from ATCC (Manassas, VA, USA). MDA-MB-361 and HCC-38 cells were routinely cultured in DMEM/F12, whereas Vero cells were grown in MEM media (Invitrogen), supplemented with 10 % FBS (Invitrogen 16000–044) and 1 % Penicillin/Streptomycin (Invitrogen 15140–122). The cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO2 and 95 % oxygen at all times. HCC38 and Vero cells were seeded in 96-well microtiter plates at density 5 × 104 cells/well, whereas MDA361 cells were plated at 1.25 × 104 cells/well and incubated overnight with respective medium described above to obtain a 70 % confluent layer. The monolayer was treated with different concentrations (3.125–25 μg/ml) of the plant extract/fractions and incubated for 48 h at 37 °C. In all experiments a negative control and a positive control were maintained. Negative control contained only growth media while the positive control contained 50 % DMSO.
4
Breast Cancer Cell Line Characterization
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All cell lines were purchased from the American Type Culture Collection (ATCC). The following Her2− human breast cancer cell lines were used: HCC38, HCC1395, HCC70, MDA231, MDA468, HCC1806, HCC1143 and HCC1937. The following HER2+ breast cancer cell lines were used: HCC202, HCC1569, HCC1419, BT474, UACC893, CRL2351 and HCC1954. UACC893, MDA468 and BT474 cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Hyclone), and 1% penicillin/streptomycin (P/S). All other cancer cell lines (HCC38, HCC1937, HCC1395, HCC70, HCC1806, HCC1143, HCC202, HCC1569, HCC1419, CRL2351, HCC1954 and MDA231) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen), supplemented with 10% FBS and 1% P/S. MCF10A cells were grown in DMEM/F12 medium supplemented with 5% horse serum, 10 μg/ml insulin, 20 ng/ml EGF, 0.5 μg/ml hydrocortisone, 100 ng/ml Cholera toxin and 1% P/S. Cells were grown at 37°C in 5% CO2. Retroviral and lentiviral infections were performed as previously described [35 (link)].
5
Culturing TNBC Cell Lines
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The HCC38 and MDA-MB-468 TNBC cell lines were purchased from the American Type Culture Collection (LGC Standards, Molsheim, France). MDA-MB-468 cells were maintained in RPMI-1640 containing Glutamax (Invitrogen) and supplemented with 10% FBS. HCC38 cells were cultured in RPMI-1640 containing Glutamax and supplemented with 10% FBS, 1.5 g/L sodium bicarbonate, 10 mM Hepes (Invitrogen) and 1 mM sodium pyruvate. Antibiotics were added to all media (100 U/mL penicillin and 100 μg/mL streptomycin). Cells were cultured at 37°C in a damp incubator, under an atmosphere containing 5% CO2.
6
Cell Line Culture and Maintenance
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HEK293T, MCF10A, MCF12A, 184-B5, MDA-MB-468, MDA-MB-453, HCC1937, HCC38, MCF-7, and MDA-MB-231 were obtained from the American Type Culture Collection (Manassas, VA). The KLF4+/+ MEF and KLF4−/− MEF were gifts from Dr. Engda Hagos (Colgate University). HEK293T, MDA-MB-468, MDA-MB-453, HCC1937, HCC38, MCF-7, MDA-MB-231, KLF4+/+ MEF and KLF4−/− MEF were maintained in DMEM supplemented with 5 or 10% FBS, 1× antibiotic/antimycotic solution (100 units/ml streptomycin and 100 units/ml penicillin) (all from Invitrogen). MCF10A, MCF12A, 184-B5 were maintained in monolayer in DMEM/F12 supplemented with 5% horse serum (Gibeco), 10 μg/ml insulin (Sigma), 20 ng/ml EGF (Sigma), 0.5 μg/ml hydrocortisone (Sigma), 100 ng/ml cholera toxin (Sigma) and 1× antibiotic/antimycotic solution.
7
Comparative Cytotoxicity Assay Protocol
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DAPT and gliotoxin, were obtained from Merck Millipore (Darmstadt, Germany). The compounds were reconstituted in dimethyl sulfoxide (DMSO). Human cell lines derived from melanoma (518A2), HCC (HEP3B, SNU398, Huh7), pancreas-CA (PANC1), and breast-CA (HCC38, MDA-MB-468) were cultured in RPMI 1640 supplemented with 10% heat inactivated fetal calf serum (FCS), 2 mM Glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin (all reagents were obtained from Gibco, Life Technologies Inc., Paisley, United Kingdom). HEP3B (HB-8064), SNU398 (CRL-2233), PANC1 (CRL-1469), HCC38 (CRL-2314), and MDA-MB-468 (HTB-132) cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MA, United States). The cell line Huh7 (JCRBO 403) was obtained from the National Institute of Biomedical Innovation (Osaka, Japan). The melanoma cell line 518A2, characterized by the BRAF V600E mutation and a CDKN2A exon 2 deletion, was obtained from Leiden University. The generation of the HCC cell line HCC-3 was described previously (Winter et al., 2008 (link)). Cells were incubated with the indicated concentrations of inhibitors or with equal amounts of solvent.
8
Cell Culture Conditions for Breast Cancer
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The HCC38, MDA-MB-468, MDA-MB-231, MCF7, MCF10A cells were purchased from the American Type Culture Collection (ATCC). HCC38 cells were cultured in RPMI 1640 medium (GIBCO, C11875500BT) supplemented with 10% fetal bovine serum (FBS). MCF10A cells were cultured in RPMI 1640 medium supplemented with horse serum (5%), insulin (10μg/mL) and epidermal growth factor (20 ng/mL), hydrocortisone (0.5μg/mL). MCF7, MDA-MB-231 and MDA-MB-468 cells were grown in Dulbecco’s modified Eagle’s medium (GIBCO, C11995500BT) supplemented with 10% FBS at 37 °C and 5% CO2,100 units per mL penicillin (MDbio, P003-10 g), and 100 mg/mL streptomycin (MDbio, S007-25 g).
9
Culturing Diverse Breast Cell Lines
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Human normal breast epithelial cells (MCF-10A) and BRCA cell lines (MCF-7, MDA-MB-231, MDA-MB-468, HCC1954, HCC38, SK-BR3, and T47D) were obtained from American Typical Culture Collection (ATCC, Manassas, VA). Normal breast MCF-10A cells were cultured in a mammary epithelium-specific medium (Lonza). MDA-MB-231 and MDA-MB-468 cells were cultured in sealed medium containing L-15 basal medium (Solarbio). MCF-7 cells were cultured in DMEM medium (Gibco). HCC1954, HCC38, SK-BR3, and T47D cells were cultured in 1640 medium (Gibco). In addition to the above dedicated medium, the addition of 10% fetal bovine serum (VivaCell) was essential, while penicillin/streptomycin (Hyclone) was selectively added according to the cell status. Growth conditions for all cells were 37°C and 5% CO2 concentration.
10
Culturing Breast and Kidney Cell Lines
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The basal‐like breast cancer cell lines MDA‐MB‐468, HCC70, HCC38, the luminal breast cancer line MCF7, and the human embryonic kidney (HEK) 293T cells were originally obtained from the American Type Culture Collection (USA). MDA‐MB‐468, HCC70, HCC38, and MCF7 were grown in RPMI, while HEK293T cells were grown in Dulbecco's modified Eagle medium (DMEM) (Gibco BRL, USA). Unless otherwise indicated, all cell lines were cultured in a medium containing 10% fetal bovine serum (Gibco BRL, USA), L‐Glutamine (2 mM), and penicillin/streptomycin. Cells were cultured at 37 °C in a humidified incubator of 5% CO2. Cell lines were routinely (once a month) checked for mycoplasma using an RT‐PCR.
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