Pe20 tubing

For experiments requiring intracerebroventricular (i.c.v.) drug infusion, pre-canulated (third ventricle) C57BL/6J mice were received from The Jackson Laboratory. Animals had internal guide cannula (2.5 mm long; Plastics One, USA) mounted in the 3rd ventricle (standard coordinates—ML: +1.0, RC: −0.4, DV: 2.0 mm) and protected by a dummy cap until the experiment. For more information on the cannulation procedure, animal care, and use, see the link: https://www.jax.org/-/media/jaxweb/files/jax-mice-and-services/brain-cannulation-information-care-use.pdf?la=en&hash=FD75F73AB0CD7A47808C78D0FC405AB3AF123F3B (accessed on 14 June 2021).
Conscious freely moving mice were infused i.c.v. with rimonabant (2 µg), JD5037 (1 µg), or their solvents. Drugs were applied in a volume of 1 μL over the course of 2 min via 33 g internal injector (P1 Technologies, Roanoke, VA, USA) connected with the 2 µL precision glass Hamilton syringe (USA) by a PE-20 tubing (Fisher Scientific, Hampton, NH, USA). The infusion rate and volume were controlled through the use of the syringe pump (model PHD 22/2000, Harvard Apparatus, Cambridge, MA, USA). Following 2 min infusion, injectors were left in place for additional 3 min to allow a passive diffusion of drugs into the tissue.