Tgbc1tkb

A panel of five cell lines of Asian origin was used for comparison of doubling times and drug sensitivity. GB-d1 [14 (link)] was provided by Dr. Anirban Maitra (Department of Pathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA); NOZ was obtained from the Health Science Research Resources Bank (Osaka, Japan; No JCRB1033); and G-415, TGBC-1TKB and TGBC-2TKB were purchased from RIKEN BioResource Center (Ibaraki, Japan; No RCB2640, RCB1129 and RCB1130). G-415 and GB-d1 were grown in RPMI 1640 medium (Thermo Scientific HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS), 10 units/mL penicillin and 10 mg/mL streptomycin (1% penicillin/streptomycin, Thermo Scientific HyClone). NOZ, TGBC-1TKB and TGBC-2TKB were grown in Dulbecco’s Modified Eagle Medium (DMEM high glucose; Corning, New York, NY, USA) supplemented with 5% FBS and 1% penicillin/streptomycin.

Twenty human biliary tract cancer cell lines were collected from different sources. Huh-28 (ICC), HuCCT1 (ICC), OZ (CPHBD), NOZ (CGB) and OCUG-1 (CGB) cell lines were obtained from the Japanese Collection of Research Bioresources Cell Bank. TKKK (ICC), TFK-1 (CPHBD), TGBC1TKB (CGB), TGBC2 (CGB), TGBC14TKB (CGB), TGBC24TKB (CGB) and G-415 (CGB) cell lines were obtained from the RIKEN BioResource Center. SNU-1079 (ICC), SNU-245 (CPHBD), SNU-1196 (CPHBD) and SNU-308 (CGB) cell lines were obtained from the Korean Cell Line Bank. The Egi-1 (CPHBD) cell line was obtained from the Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures. All cell lines were cultured as recommended by their respective cell banks. SK-ChA-1 (CPHBD), Mz-ChA-1 (CGB), Mz-ChA-2 (CGB) were obtained from Prof. Alexander Knuth (University Hospital of Zürich, Zürich, Switzerland) (32 (link)) and cultured in RPMI 1640 with 10 mM HEPES, 2 mM L-Glutamine, 1X MEM non-essential amino acids (Thermo Fisher Scientific), 100 U/ml penicillin, 100 µg/ml of streptomycin, and 10% fetal bovine serum (FBS). Human BMSCs (196hT) immortalized with the hTERT/GFP system (33 (link)) were cultured as described previously (34 (link)). The control cell line 293/hTNW that stably expresses human tenascin-W (27 (link)) was cultured in DMEM with 0.25 µg/ml of G418, 1.5 µg/ml of puromycin, and 10% FBS.

The human GBC cell lines G-415, TGBC1TKB, TGBC2TKB were obtained from RIKEN BioResource Center (Ibaraki, Japan). NOZ was purchased from the health Science Research Resources Bank (Osaka, Japan) and GB-d1 was donated by Anirban Maitra (Department of Pathology, John Hopkins University School of Medicine, Baltimore, MD, USA). All cell lines were routinely tested for Mycoplasma by PCR and authenticated by the Dr. Justo Lorenzo Bermejo at the University of Heidelberg by short tandem repeat DNA profiling. They were used in the described experiments for a maximum of 10 passages after thawing.