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Ptc gal4

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The Ptc-Gal4 is a lab equipment product. It is a genetic construct that contains the promoter sequence of the Patched gene, which drives the expression of the Gal4 transcription factor in cells where the Patched gene is expressed.

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Lab products found in correlation

En gal4, by BD (3 mentions) Gmr gal4, by BD (2 mentions) Ey gal4, by BD (2 mentions) Gmr gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas p35, by BD (1 mentions) Uas diap1, by BD (1 mentions) Uas cyce, by BD (1 mentions) Nub gal4, by BD (1 mentions) Uas bskdn, by BD (1 mentions) Apgal4, by BD (1 mentions) Dpp gal4, by BD (1 mentions) C96 gal4, by BD (1 mentions) Act5c gal4, by BD (1 mentions) Elav gal4, by BD (1 mentions) Mef2 gal4, by BD (1 mentions) Repo gal4, by BD (1 mentions) Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions) Uas lifeactinrfp, by BD (1 mentions)

4 protocols using ptc gal4

1

Drosophila Genetics Protocol

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All Drosophila strains were grown and maintained at 25 °C. Following fly stocks were used for the experiments: Ciao1 RNAi (Vienna Drosophila Resource Center, v32020 and v105939), Xpd RNAi (Vienna Drosophila Resource Center v106998), UAS-CycE (BDSC 4781), UAS-Diap1, UAS-p35, ey-Gal4, GMR-Gal4, en-Gal4, ptc-Gal4, nub-Gal4, and FRT42D M(2)531 (BDSC 5698) were obtained from the Bloomington stock center. For overexpression of Crbintra, UAS-Crbintra was crossed with GMR-Gal4 (Bloomington). Xpdp flies were a kind donation from Dr. Beat Suter. To construct the transgenic lines, UAS-Ciao1 and UAS-Xpd, full length Ciao1 and Xpd cDNA (from the Drosophila Genome Research Center) were cloned into pUAST vector.
Jung J., Yeom E, & Choi K.W. (2020). Ciao1 interacts with Crumbs and Xpd to regulate organ growth in Drosophila. Cell Death & Disease, 11(5), 365.
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2

Drosophila Genetics and Imaging Protocols

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All fly stocks were maintained on standard cornmeal/yeast/molasses/agar medium at 25 °C as per standard procedures. Oregon-R flies were used as wild-type controls. UAS-GFP-zip, UAS-GFP-Zip DN (UAS-Myo II-Neck-Rod), UAS-Myo II-Rod, and UAS-Myo II-Rod (delta Nterm58) [26 (link)] were obtained as a gift from Prof. Daniel P. Kiehart (Department of Biology, Duke University, Durham, NC). UAS-Notch-FL [27 (link)], UAS-Notch-ICD, UAS-Notch-DN [28 (link)], and Notch pathway components were kindly provided by Prof. S. Artavanis-Tsakonas (Department of Cell Biology, Harvard Medical School, Boston, MA).

cn1 bw1 sp1 zip1/CyO (BDSC 4199),

P{FRT(whs)}G13 zip2/CyO (BDSC 8739),

P{lacZ.w+}276, y1 sc* v1 sev21; P{TRiP.GL00623}attP40 (BDSC 37480), y1 sc* v1 sev21; P{TRiP.HMS01618}attP2 (BDSC 65947),

SqhAX3-GFP (BDSC 57144), UAS-Bsk-DN (BDSC 6409), vg-GAL4 (BDSC 8222), GMR-GAL4 (BDSC 8121), ptc-GAL4 (BDSC 2017), en-GAL4 (BDSC 30564), ap-GAL4 (BDSC 56807), dpp-GAL4 (BDSC 1553), and C96-GAL4 (BDSC 43343) stocks were obtained from Bloomington stock center. All crosses were performed at 25 °C. The combination lines vg-GAL4/UAS-GFP-zip and UAS-Notch-DN/C96-GAL4 were made with the help of appropriate genetic crosses.

Verma D., Singh A., Singh J., Mutsuddi M, & Mukherjee A. (2024). Regulation of Notch signaling by non-muscle myosin II Zipper in Drosophila. Cellular and Molecular Life Sciences: CMLS, 81(1), 195.
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3

Fly Strains for Molecular Studies

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The following fly strains were used throughout the study: Act5C-Gal4 (BDSC #3954, Christian Klämbt), ey-Gal4 (BDSC #5535), ey-Gal4, UAS-Dl/CyO flies (a gift from B. Hassan), robo2-Gal4 (BDSC #48074), en-Gal4 (BDSC #6356), ptc-Gal4 (BDSC #52212), C179-Gal4 (BDSC #6450), mef2-Gal4 (BDSC #27390), CG25C-Gal4 (BDSC #7011), elav-Gal4 (BDSC #458), slit-Gal4 (BDSC #9580, Christian Klämbt), repo-Gal4 (BDSC #7415, Christian Klämbt), gcm-Gal4 (BDSC #35541), crol-Gal4 (VDRC #200123), ama-Gal4 (#205487), appl-Gal4 (a gift from Doris Kretzschmar, Münster) and tkk-Gal4 (#45606). All RNAi strains used are listed in (Table S6).
Chaouch A., Berlandi J., Chen C.C., Frey F., Badini S., Harutyunyan A.S., Chen X., Krug B., Hébert S., Jeibmann A., Lu C., Kleinman C.L., Hasselblatt M., Lasko P., Shirinian M, & Jabado N. (2021). Histone H3.3 K27M and K36M mutations de-repress transposable elements through perturbation of antagonistic chromatin marks. Molecular cell, 81(23), 4876-4890.e7.
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4

Drosophila Genetic Manipulation Protocols

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Drosophila melanogaster stocks were maintained according to protocols described in Ashburner manual (59) . Crosses were maintained at 18 0 C until the time of gene expression induction The description of mutations, insertions and transgenes is available at Fly Base (http://flybase.org).
The following drivers were used to induce ectopic expression using the Gal4/UAS (60) and QUAS-QF (61) systems: tubGal80 ts (Bloomington Drosophila Stock Center, BDSC), hh.Gal4 (62) , ptc.Gal4 (63) and Hh-QF (generated by Ernesto Sánchez-Herrero, CBMSO).
Overexpression stocks: The pUAS-transgene strains used were: UAS.ihog-YFP (64) and UAS.LifeActinRFP (BDSC 58362). The QUAS-transgene strains used were:
Aguirre‐Tamaral A., & Guerrero I. (2021). Improving the understanding of cytoneme-mediated morphogen gradients by in silico modeling.
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