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Ly2606368

Manufactured by Selleck Chemicals
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LY2606368 is a chemical compound that functions as a selective inhibitor of the protein kinase Chk1. It is commonly used in research applications to study cell cycle regulation and DNA damage response pathways.

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Lab products found in correlation

Azd6738, by Selleck Chemicals (3 mentions) Etoposide, by Merck Group (2 mentions) Hydroxyurea, by Merck Group (2 mentions) Ku55933, by Selleck Chemicals (2 mentions) Ve 822, by Selleck Chemicals (2 mentions) Azd0156, by Selleck Chemicals (2 mentions) Nu7441, by Selleck Chemicals (2 mentions) Mitomycin c, by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) Camptothecin, by Merck Group (1 mentions) Olaparib, by Abcam (1 mentions) Recombinant human ifn γ, by Thermo Fisher Scientific (1 mentions) Carboplatin, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) Azd6738, by AstraZeneca (1 mentions) Ly2603618, by Selleck Chemicals (1 mentions) Azd1775, by AstraZeneca (1 mentions) Mk 1775, by MedChemExpress (1 mentions) Nutlin 3a, by MedChemExpress (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

8 protocols using ly2606368

1

Evaluating Small Molecule Inhibitors

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KU-55933, AZD6738, and LY2606368 were purchased from Selleck Chemicals. Olaparib was purchased from BioVision Inc. Hydroxyurea, cisplatin, mitomycin C (MMC), camptothecin, and etoposide were purchased from Sigma-Aldrich.
Su D., Feng X., Colic M., Wang Y., Zhang C., Wang C., Tang M., Hart T, & Chen J. (2020). CRISPR/CAS9-based DNA damage response screens reveal gene-drug interactions. DNA repair, 87, 102803.
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2

Generation and Characterization of Cell Lines

Cited 4 times
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Cell line origins and their cell growth media can be found in Supplementary Methods Table S1. mESC, FaDu and A549 ATM-knockout cell lines were generated as described before (37 (link),38 (link)). mESC Cdc25a KO clones #4/5 were kindly provided by Oscar Fernández-Capetillo (24 (link)) and U2-OS T-Rex GFP-RNase H1(D210N) (RNH1(D210N)-GFP) or GFP-RNase H1 (RNH1-GFP) cells by Pavel Janscak (39 (link)). mESCs were cultured on 0.1% gelatin-coated tissue culture flasks, and Cas9-expressing cell lines were maintained in blasticidin (10 μg/ml mESC and U2-OS, 5 μg/ml HAP-1). AZD6738 and AZD1775 were made by AstraZeneca. DRB (5,6-dichloro-1-beta-ribo-fuanosyl benzimidazole), actinomycin D, aphidicolin, hydroxyurea, carboplatin and etoposide were obtained from Sigma-Aldrich. XL413 (S7547), VE-822 (S27102), LY2603618 (S2626) and LY2606368 (S7178) were obtained from SelleckChem. CX-5461 (509265) was obtained from Merck Millipore, and BRD-6989 (6438) from Tocris. Final assay DMSO concentrations were normalised to 1:1000 as required. Recombinant human IFNγ was obtained from Peprotech (300–02) and used at a final concentration of 2.5 ng/ml.
Lloyd R.L., Urban V., Muñoz-Martínez F., Ayestaran I., Thomas J.C., de Renty C., O’Connor M.J., Forment J.V., Galanty Y, & Jackson S.P. (2021). Loss of Cyclin C or CDK8 provides ATR inhibitor resistance by suppressing transcription-associated replication stress. Nucleic Acids Research, 49(15), 8665-8683.
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3

Inhibitors for Cancer Research

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MK1775 (WEE1 inhibitor) was purchased from MedChemExpress. FTD/TPI (TAS‐102) was provided by Taiho Pharmaceutical Co., Ltd. PD0166285 was purchased from AdooQ BioScience. LY2606368 (CHK1 inhibitor) was purchased from Selleck Chemicals. Nutlin‐3a (MDM2 inhibitor) was purchased from MedChemExpress.
Nguyen Vu T.H., Kikuchi O., Ohashi S., Saito T., Ida T., Nakai Y., Cao Y., Yamamoto Y., Kondo Y., Mitani Y., Kataoka S., Kondo T., Katada C., Yamada A., Matsubara J, & Muto M. (2023). Combination therapy with WEE1 inhibition and trifluridine/tipiracil against esophageal squamous cell carcinoma. Cancer Science, 114(12), 4664-4676.
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4

Small Molecule Inhibitor Compound Sourcing

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AZD6738, VE822, NU7441, AZD0156, and LY2606368 were all purchased from Selleck Chemicals.
Wang C., Wang G., Feng X., Shepherd P., Zhang J., Tang M., Chen Z., Srivastava M., McLaughlin M.E., Navone N.M., Hart G.T, & Chen J. (2018). Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene, 38(14), 2451-2463.
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5

Cell Culture and Compound Treatments

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All cells purchased from ATCC were maintained in the recommended cell culture media at 37 °C in a 5% CO2 humidified atmosphere. Specifically, HEK293T, HeLa, DU145, and 22Rv1 cells were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific), while PC-3 cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with L-glutamine. All media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. Cells were treated with various compounds, including 10 μM MG132 (cat. no.: C2211, Sigma-Aldrich), 50 μM chloroquine (cat. no.: C6628, Sigma-Aldrich), 10 μg/mL cycloheximide (cat. no.: 01810, Sigma-Aldrich), 1 μM MLN4924 (cat. no.: 505477, Calbiochem, San Diego, CA, USA), 5 μM GF109203X (cat. no.: S7208, Selleckchem, Houston, TX, USA), 5 μM AZD1208 (cat. no.: S7104, Selleckchem), 5 μM AZD7762 (cat. no.: S1532, Selleckchem), 5 μM LY2606368 (cat. no.: S6385, Selleckchem), and 300 nM Taxotere (docetaxel trihydrate, Sanofi-Aventis Deutschland GmbH, Frankfurt, Germany), as indicated.
Lee C.J., Lee H., Kim S.R., Nam S.B., Lee G.E., Yang K.E., Lee G.J., Chun S.H., Kang H.C., Lee J.Y., Lee H.S., Cho S.J, & Cho Y.Y. (2024). ELK3 destabilization by speckle-type POZ protein suppresses prostate cancer progression and docetaxel resistance. Cell Death & Disease, 15(4), 274.
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6

Detailed Chemical Inhibitor Library

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Chemical compounds including AZD1775, THZ1 2HCl, TWS119, PD-0332991, RO-3306, PD0166285, LJI308, ON-01910, LY2606368, MK-2206 2HCl, AMN-107, VE-821, KU55933, KU-57788, NSC 119875, and NSC 125973 were acquired from Selleck. Protein kinase inhibitor 1 hydrochloride was sourced from MCE. Supplementary Table 3 provides IC50 values for 15 inhibitors obtained from cell-free assays, including information on their target specificity, potential secondary targets, as well as their commercial names and catalog numbers. To supply nucleotide precursors, a mix of four dNTPs (50 uM each) was added for 48 h. The dNTP set from Sigma-Aldrich (DNTP100-1KT) consists of four separate 0.25 mL tubes, each containing 100 mM aqueous solutions of deoxyribonucleotides: deoxyribose adenosine triphosphate, deoxyribose cytidine triphosphate, deoxyribose guanosine triphosphate, and deoxyribose thymidine triphosphate.
Yang X., Hu X., Yin J., Li W., Fu Y., Yang B., Fan J., Lu F., Qin T., Kang X., Zhuang X., Li F., Xiao R., Shi T., Song K., Li J., Chen G, & Sun C. (2024). Comprehensive multi-omics analysis reveals WEE1 as a synergistic lethal target with hyperthermia through CDK1 super-activation. Nature Communications, 15, 2089.
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7

Small Molecule Inhibitor Compound Sourcing

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AZD6738, VE822, NU7441, AZD0156, and LY2606368 were all purchased from Selleck Chemicals.
Wang C., Wang G., Feng X., Shepherd P., Zhang J., Tang M., Chen Z., Srivastava M., McLaughlin M.E., Navone N.M., Hart G.T, & Chen J. (2018). Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene, 38(14), 2451-2463.
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8

Apoptosis-inducing Reagents Protocol

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The following reagents were used for cells: MG132 (Selleck; S2619), AZD1390 (Selleck; S8680), LY2606368 (Selleck; S7178), ADR (Selleck, S1208), ActD (APExbio, A4448), etoposide (Selleck; S1225), and camptothecin (Selleck; S1288).
Lin X., Zhou L., Zhong J., Zhong L., Zhang R., Kang T, & Wu Y. (2021). RNA-binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53. The Journal of Biological Chemistry, 298(2), 101524.
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