Gcms qp5050

(A) Mass spectroscopy: mass spectroscopy was carried out using Direct Inlet Unit (DI-50) of SHIMADZU GC/MS-QP5050 A. Software: Class 5000. Ionization model: EI. Ionization voltage: 70 ev. Scan speed: 2000 amu/sec. Scan interval: 0.5 sec, at the Regional Center for Mycology and Biotechnology, Al-Azhar University
(B) Infrared (IR) spectra: infrared spectrum of the new antimicrobial agent was conducted in potassium bromide using Fourier Transform infrared and Pye Unicam SP300 IR spectrophotometer at the Micro-Analytical Center, Cairo University
(C) The proton nuclear magnetic resonance (¹H-NMR): ¹H-NMR was conducted in deuterated chloroform using Varian Gemini 200 and 300 MHz NMR spectrophotometer at the Micro-Analytical Center, Cairo University.

About 1.5 mg of the sample PU-3:1 was pressed in a special syringe and directly pyrolyzed at 450 °C in a Pyrojector II (SGE Analytical Science, Trajan Scientific and Medical, Melbourne, Australia). The pyrolysis chamber was maintained at 450 °C. Pyrolysis products were separated in a GCMS-QP5050 instrument (Shimadzu Corporation, Kyoto, Japan). Helium was the carrier gas at a pressure of 100 kPa in the pyrolyzer and 70 kPa in the GC injector (280 °C, 1–20 split ratio). The initial temperature in the oven was 45 °C for 4 min, then increased to 240 °C at a rate of 4 °C min⁻¹ and finally until 280 °C at a rate of 39 °C min⁻¹. Pyrolysis products were identified by mass spectra interpretation and comparison with NIST and Wiley computer libraries and reference literature [28 (link),29 (link)]. For each pyrogram, normalized at the most intense peak, the relative peak areas of 26 principal phenolic lignin pyrolysis products as well as the respective PU pyrolysis products were determined.

All utilized starting ingredients—benzoyl isothiocyanate, 2,6-diaminopyridine, metallic salts, and solvents—were bought from viable vendors (Merck or Sigma-Aldrich) and used instantly with no extra purifications. The molecular structures of the isolated substances have been clarified through the subsequent strategies:

C, H, N, and S content	C, H, N, and S contents were obtained using Thermo-Fisher Scientific Analyzer Model: Flash 2000
Metal and Cl¯ contents	Using described approaches in Vogel's Textbook of Quantitative Chemical Analysis18
UV–Vis	Unicam UV–Vis spec. in 1 × 10−4 M concentration in DMSO solvent
FT-IR spectra	Mattson 5000 (4000–400cm−1, KBr discs)
Mass spectra	DI-50 unit-Shimadzu GC–MS-QP5050A
Magnetic moment	Sherwood magnetic balance at 32 °C
1H, 13C-NMR	JEOL ECA-500 II
P-XRD	Shimadzu XRD 6000 diffractometer (Japan), Cu anode, Ka: 0.154060 nm, 2θ = 5–80°
TGA	Perkin Elmer TGA 4000, 30–900 °C, N2 flow 20 ml/min, Rate of heating 10 °C/min
Morphology studies	SEM, JOEL JSM 6510 lv. TEM, JOEL JEM 2100

The liver lipid extraction was obtained according to the previously described method [22 (link)]. Briefly, the extracted fat was saponified (10 min. at 75°C) with a 0.5 M solution of KOH/MetOH and subjected to methylation (10 min. at 75°C) in 14% (v/v) BF₃/MetOH (Sigma-Aldrich, St. Louis, MO, USA). Then, the methyl esters of fatty acids were extracted with hexane (Avantor Performance Materials Poland S.A., Gliwice, Poland) and analysed using a gas chromatograph coupled with a mass spectrometer (Shimadzu GCMS QP 5050, Shimadzu, Kyoto, Japan). Separation was achieved using SP-2560 capillary column with a length of 100 m, inner diameter of 0.25 mm, and film thickness of 0.25 μm (Supelco, St. Louis, MO, USA). Helium was the carrier gas.
Identification of methyl esters of long-chain fatty acids was based on the reference standards (FAME Mixture, Larodan Fine Chemicals, Malmo, Sweden) and a library of mass spectra (NIST 1.7). Percentage of methyl esters of fatty acids was calculated from the analytical signal with the formula (A_i/∑_iA) · 100 where A_i is the ith signal of the ester and ∑_iA is the sum of all identified analytical signal esters [23 (link)].