Mice containing a loxP-flanked Cd200 conditional allele (Cd200fl) were re-derived from frozen sperm (B6NTac;B6N-Cd200tm1a(KOMP)Wtsi/H) (EUCOMM MRC Harwell Repository). Mutant mice exhibiting germline Cd200fl transmission were backcrossed ten generations onto a FVB/N genetic background (Taconic Biosciences). Epidermal-targeted Cd200 conditional null mice were generated by crossing Cd200fl/fl mice with Krt14Cre transgenic mice [36 (link)] (B6N.Cg-Tg(KRT14-cre)1Amc/J; The Jackson Laboratory) to generate Krt14Cre;Cd200fl/fl (EpiKO) mice (Supplemental Figure 1A ). Wild-type animals used were FVB/N (Taconic Biosciences) and C57Bl/6 (Jackson Laboratories). Nu/J immunocompromised mice (The Jackson Laboratory) were chosen for orthotopic studies as they harbor the same MHC H2 haplotype, H-2Kq, as the FVB/N mouse strain. All animals were housed under pathogen-free conditions and received standard rodent chow and water ad libitum according the Institute of Comparative Medicine guidelines. All experiments involving mice were conducted under Columbia University IACUC-approved protocols.
Fvb n
Manufactured by Taconic Biosciences
The FVB/N is a mouse strain primarily used in scientific research. It is an inbred strain that exhibits characteristics such as rapid breeding, ease of handling, and responsiveness to treatment. This mouse strain is frequently employed in various areas of biomedical research, including the study of gene expression, disease models, and transgenic technology.
Automatically generated - may contain errors
Lab products found in correlation
Balb c, by Taconic Biosciences (1 mentions)
C57bl 6, by Taconic Biosciences (1 mentions)
Fvb n, by Jackson ImmunoResearch (1 mentions)
Nu j immuno compromised mice, by Jackson ImmunoResearch (1 mentions)
B6n cg tg krt14 cre 1amc j, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
4 protocols using fvb n
1
Conditional CD200 Knockout Mice
2
Trpv6 Knockout Mice and Early Weaning Protocol
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FVB/N (Taconic Biosciences, Rensselaer, NY) and Trpv6mt mice24 (link) were maintained on a 12-hour light/dark cycle with drinking water and chow ad libitum (Lab Diet Irradiated Rodent Diet 5053, 4% fat, 0.81% calcium). Experiments were approved by the University of Alberta animal ethics committee, Health Sciences Section (AUP00000213). Experiments on the Cacna1d and HA-tagged Cacna1d KO mice16 (link) were conducted in agreement with the European Communities Council Directive (2010/63/EU) in accordance with the German law on the use of laboratory animals and approved by the regional board for scientific animal experiments of Saarland. Trpv6mt mice were genotyped by polymerase chain reaction (PCR).24 (link), 51 (link) For the early weaning experiments, half of the mice in a litter (FVB/N mice) were weaned at P12 to the standard rodent chow diet, and the littermates remained with the dam. After 48 hours, tissue was collected from all pups. Experiments involving mice included both female and male mice in approximately equal numbers except for the bone phenotype analysis of P14 Trpv6mt mice.
3
Scapular Mammary Tissue Transplantation
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Recipient female mice (FVB/N, Taconic) at 8–9 weeks of age were anesthetized (isoflurane/oxygen) and the scapular region shaved. 24 h later, whole inguinal mammary tissue lacking associated lymph nodes was harvested from donor females and placed subcutaneously into the scapular region of recipient females under aseptic conditions. Incisions were closed using a 9 mm wound clip, which was removed 10 days post-transplant. Recipient animals were monitored biweekly for ~10 months, at which the mice were sacrificed and mammary tissue harvested for histology.
4
Mouse Breeding and Pathogen-Free Maintenance
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C57BL/6, FVB/N, and BALB/c mice were purchased from Taconic Farms. All animals used in this study were males, 8–10 weeks of age. Animals were bred and maintained under specific pathogen‐free (SPF) conditions in Thoren Isolator racks under positive pressure. The Institutional Care and Use Committee of the University of Alabama at Birmingham (UAB) approved all experiments. SPF conditions at UAB include an absence of the following organisms, as determined by serological screening: mouse parvoviruses, including MPV‐1, MPV‐2, and minute virus of mice; mouse hepatitis virus, murine norovirus, Theiler's murine encephalomyelitis virus; mouse rotavirus (epizootic diarrhea of infant mice), Sendai virus; pneumonia virus of mice; reovirus; Mycoplasma pulmonis; lymphocytic choriomeningitis virus; mouse adenovirus; ectromelia (mousepox) virus; K polyomavirus; and mouse polyomavirus. Testing and other methods were as described at http://main.uab.edu/Sites/ComparativePathology/surveillance/ .
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