Gibco dmem glutamax

Manufactured by Thermo Fisher Scientific

Sourced in United States

Gibco DMEM GlutaMAX is a cell culture medium formulation designed to support the growth and maintenance of various cell types. It contains the amino acid L-glutamine, which is a key nutrient for cell metabolism.

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Lab products found in correlation

Penicillin streptomycin, by Merck Group (1 mentions) Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Prl tk, by Promega (1 mentions) Thapsigargin, by Merck Group (1 mentions) Lipofectamine ltx plus, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Tryptose phosphate broth, by Teknova (1 mentions) Gibco heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Gibco sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Pyruvate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

GlutaMAX (10236 citations) DMEM GlutaMAX (797 citations) Gibco DMEM (115 citations) Gibco GlutaMAX (37 citations) GIBCO® DMEM/F12 GlutaMAX™-I, (3 citations)

4 protocols using gibco dmem glutamax

Vinculin-eGFP expressing cell lines

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RPE1 wild type (WT) (provided by L. Blanchoin, Cytomorpholab Grenoble, France and stably transfected with vinculin-eGFP by Y. A. Miroshnikova, Institute of Advanced Biology, France), NIH-3T3 WT (gift by H. Maiato, University of Porto, Portugal and stably transfected with vinculin-eGFP by Y. A. Miroshnikova, Institute of Advanced Biology, France), and NIH-3T3 optoGEF_RhoA (given by M. Coppey, Institute Curie, France) were cultured under standard cell culture conditions (37°C, 5% CO₂) in Gibco Dulbeccoʼs Modified Eagle Medium/Nutrient Mixture F-12 (DMEM)/F-12 GlutaMAX and Gibco DMEM GlutaMAX (Life Technologies), respectively, containing 10% heat-inactivated fetal bovine serum (Life Technologies) and penicillin/streptomycin (100 μg/ml) (Sigma-Aldrich).
Cells were plated on patterned polyacrylamide (PAA) hydrogels at a low density of 6 × 10³ cm⁻² and allowed to spread for 2 to 4 hours. For life imaging, the medium of NIH-3T3 WT/vin-eGFP/optoGEF_RhoA was replaced by Leibovitz’s L-15 medium (Life Technologies).

Hennig K., Wang I., Moreau P., Valon L., DeBeco S., Coppey M., Miroshnikova Y.A., Albiges-Rizo C., Favard C., Voituriez R, & Balland M. (2020). Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines. Science Advances, 6(1), eaau5670.

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Mouse Ophn1 5'UTR-Luciferase Reporter Assay

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The 5′ UTR of mouse Ophn1 mRNA was amplified from total RNA extracted from adult mouse brain. Primer sequences based on NCBI Reference Sequence (NM_05 2976.3) were as follows: sense 5′-CTAGCTAGCAGTTTCCGTAGGGAAGCGC-3′ and antisense 5′-TAGGCTAGCATACTTG AGCCTCTGGCGG-3′. The PCR product was then cloned into the pCIneo-Luciferase (Fluc) reporter vector. The resulting 5′UTR-OPHN1-Fluc construct was confirmed by DNA sequencing. HEK293T cells were grown in 12-well plates in Gibco DMEM+Glutamax (Life Technologies) supplemented with 10% FBS, 100 units of penicillin/streptomycin per ml. 5′UTR-OPHN1-Fluc or control Fluc vector were transfected with Renilla luciferase plasmid pRL-TK (Promega, Madison, WI) into HEK293T cells at 50–80% confluency using Lipofectamine LTX plus (Life Technologies). HEK293T cells were treated 24 h after transfection with 150 nM thapsigargin (Sigma) or vehicle (0.1% DMSO) for 6 h. Cell extracts were prepared in passive buffer and assayed for Rluc (internal control) and Fluc activity using a dual-luciferase reporter assay system (Promega). The ratio of Fluc to Rluc activity was reported as relative light units (RLU).

Viana Di Prisco G., Huang W., Buffington S.A., Hsu C.C., Bonnen P.E., Placzek A.N., Sidrauski C., Krnjević K., Kaufman R.J., Walter P, & Costa-Mattioli M. (2014). Translational control of mGluR-dependent long-term depression and object-place learning by eIF2α. Nature neuroscience, 17(8), 1073-1082.

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Culturing Mouse Glioblastoma and Hamster Kidney Cells

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GL261 mouse GBM cells (gift from Dr. G. Sáfrány, National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary) were cultured in Gibco DMEM GlutaMAX (Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% Gibco heat-inactivated fetal bovine serum (FBS, Thermo Fischer Scientific), 10 U/mL Gibco penicillin-streptomycin (PEST, Thermo Fisher Scientific), and 1 mM Gibco sodium pyruvate (Thermo Fisher Scientific). Baby hamster kidney (BHK)-21 cells were cultured in Gibco Glasgow’s minimum essential medium (GMEM, Thermo Fisher Scientific) supplemented with 10% FBS, 2% tryptose phosphate broth (Teknova, Hollister, CA, USA), 20 mM HEPES, and 10 U/mL Gibco PEST (Thermo Fisher Scientific).

Martikainen M., Ramachandran M., Lugano R., Ma J., Martikainen M.M., Dimberg A., Yu D., Merits A, & Essand M. (2021). IFN-I-tolerant oncolytic Semliki Forest virus in combination with anti-PD1 enhances T cell response against mouse glioma. Molecular Therapy Oncolytics, 21, 37-46.

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3T3-L1 Preadipocyte Culture and Differentiation

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White 3T3-L1 preadipocyte cells were cultured Gibco DMEM GlutaMAX™ (Thermo Fisher Scientific Inc., USA) culture medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin (Thermo Fisher Scientific Inc.), and 1% pyruvate (Thermo Fisher Scientific Inc.) at 37 °C and 5% CO₂. 3T3-L1 differentiation and Oil-Red-O staining were performed as previously described [47 (link)].

Hauffe R., Rath M., Schell M., Ritter K., Kappert K., Deubel S., Ott C., Jähnert M., Jonas W., Schürmann A, & Kleinridders A. (2021). HSP60 reduction protects against diet-induced obesity by modulating energy metabolism in adipose tissue. Molecular Metabolism, 53, 101276.

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