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Gelatin from porcine skin g1890

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Gelatin from porcine skin (G1890) is a laboratory reagent used in various applications. It is a protein substance derived from the collagen in porcine (pig) skin. This product can be used in various experimental procedures, but a detailed description of its intended use would require further information.

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Lab products found in correlation

Pluronic f 127, by Merck Group (2 mentions) Hematoxylin h3136, by Merck Group (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Prestoblue, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Gelatin (1674 citations) Gelatin from porcine skin (105 citations) Porcine gelatin (96 citations) G1890 (82 citations) Porcine skin gelatin (46 citations) Porcine skin (7 citations)

5 protocols using gelatin from porcine skin g1890

1

Histological Tissue Preparation Protocol

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Gelatin from porcine skin (G1890), methanol, ethanol, xylene, acetonitrile, 2,5-dihydroxyacetophenone (DHA), trifluoroacetic acid (TFA), (Hydroxypropyl)-methylcellulose (HPMC, H8384), polyvinylpyrrolidone (PVP, PVP360), and hematoxylin (H3136) were from Sigma/Aldrich (St. Louis, MO, USA). Eosin-Y solution (314–630) was from FisherSci (Pittsburgh, PA, USA). 2, 5-dihydroxybenzoic acid (DHB, D0569) was from Tokyo Chemical Industry (TCI America, Portland, OR, USA) and was recrystallized twice with water. Carboxymethylcellulose sodium (CA192) was from Spectrum Chemical (New Brunswick, NJ, USA). Indium tin oxide (ITO)-coated microscope slides (CG-81IN-S115) were from Delta Technologies (Loveland, CO, USA). The optimal cutting temperature compound (O.C.T., Tissue-Tek, 4583) was from VWR (Radnor, PA, USA).
Yang J., Rendino L., Cassar S., Buck W., Sawicki J., Talaty N, & Wagner D. (2022). Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging. Pharmaceuticals, 15(10), 1230.
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2

Pectin-Gelatin Hydrogel Synthesis

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Low methoxy pectin was obtained from Willpowder (20.4% esterification degree, Miami Beach, FL, USA). Gelatin from porcine skin (G1890) and Pluronic® F-127 (P2443) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, 22980) and 2-morpholinoethanesulfonic acid (MES, M0606) were attained from Thermo Fisher Scientific. All materials were used as received.
Kanungo M., Wang Y., Hutchinson N., Kroll E., DeBruine A., Kumpaty S., Ren L., Wu Y., Hua X, & Zhang W. (2021). Development of Gelatin-Coated Microspheres for Novel Bioink Design. Polymers, 13(19), 3339.
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3

Fluorescent Gelatin Perfusion Technique

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The method of fluorescent gelatin perfusion has been described previously [23 (link),28 (link),29 (link)]. In brief, mice were terminally anesthetized prior to infusion with IM ketamine/xylazine until the mouse no longer responded to painful stimuli. The mice were then infused with 5 mL of 2% gelatin and a mixture of heparin (1:5000; Bodene (Pty), Port Elizabeth, South Africa), atropine sulfate (1:10,000; Teva Pharmaceuticals, Petach Tikva, Israel), and fluorescein-conjugated bovine serum albumin (FITC-BSA). Thereafter, the mice were infused with 6 mL of 4% gelatin (Gelatin from porcine skin, #G1890; Sigma, St. Louis, MO, USA), together with the previous mixture. To coagulate the perfusions, the mice were placed in ice. The eyes were then enucleated and fixed immediately in 10% neutral buffered formalin in 0.1 M phosphate-buffered saline (PBS) at room temperature, and perfusion of the vasculature was examined.
Azrad-Leibovich T., Zahavi A., Gohas M.F., Brookman M., Barinfeld O., Muhsinoglu O., Michowiz S., Fixler D, & Goldenberg-Cohen N. (2022). Characterization of Diabetic Retinopathy in Two Mouse Models and Response to a Single Injection of Anti-Vascular Endothelial Growth Factor. International Journal of Molecular Sciences, 24(1), 324.
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4

Nanofiber Scaffold Fabrication and Characterization

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Poly (caprolactam) (nylon 6, 181110), formic acid (FA, F0507), thrombin from bovine plasma (T4648), fibrinogen from bovine plasma (F8630), streptozotocin (STZ, S0130) and gelatin from porcine skin (G1890) were purchased from Sigma-Aldrich Co. (St. Louis, MO). 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP, 003409) was purchased from Oakwood Products, Inc. (Estill, SC). Calcium chloride (CaCl2, BDH9224) and sodium chloride (NaCl, BDH9286) were purchased from VWR International (Radnor, PA). Barium chloride dihydrate (BaCl2.2H2O, BX0060-1) was purchased from EMD Millipore Corporation. Regular sodium alginate (PROTANAL LF 10/60FT) and sterile sodium alginate (Pronova SLG 100) were purchased from FMC BioPolymer Co. (Philadelphia, PA). Sucrose (8360-6) was purchased from Avantor Performance Materials, LLC. (Center Valley, PA). All reagents were used without further purification. Sulfobetaine-modified alginate (SB-alginate) was synthesized according to a previously published protocol[18 (link)].
Liu W., Flanders J.A., Wang L.H., Liu Q., Bowers D.T., Wang K., Chiu A., Wang X., Ernst A.U., Shariati K., Caserto J.S., Parker B., Gao D., Plesser M.D., Grunnet L.G., Rescan C., Carletto R.P., Winkel L., Melero-Martin J.M, & Ma M. (2021). A safe, fibrosis-mitigating, and scalable encapsulation device supports long-term function of insulin-producing cells. Small (Weinheim an der Bergstrasse, Germany), 18(8), e2104899.
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5

Biomimetic Cell Culture Scaffold

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Cellulose fiber (Specific gravity =1.27-1.6 g/cm 3 , diameter = 20 µm, length = 500, 1000 and 2000 µm) was provided by Weidmann Fiber Technology. PE fiber (Specific gravity = 0.96 g/cm 3 , diameter = 15 µm, length = 600 µm) was provided by Minifiber, Inc. Fibrinogen (F8630) and thrombin (T4648) from bovine plasma, gelatin from porcine skin (G1890) and Pluronic ® F127 (P2443) were purchased from Sigma-Aldrich. Alexa Fluor™ 488 Phalloidin (Cat. #A12379) was purchased from ThermoFisher. PrestoBlue (Cat. #A13261) was purchased from Invitrogen. Goat anti-mouse CD31 (Cat. #AF3628) was purchased from R&D systems. Alexa Fluor TM 488 donkey anti-goat antibody was purchased from Invitrogen (Cat. #A11055). α-SMA conjugated with Cy3 (Cat. #C6198) was purchased from Sigma. All reagents were used without further purification.
Gao D., Ernst A.U., Wang X., Wang L., Liu W, & Ma M. (2022). Engineering a Hierarchical Biphasic Gel for Subcutaneous Vascularization. Advanced healthcare materials, 11(19).
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