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Mavs mice

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The Mavs+/− mice are a genetically modified mouse model that has one of the two copies of the Mavs gene inactivated. The Mavs gene is involved in the innate immune response. This mouse model is used in research to study the role of the Mavs gene in various biological processes.

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Lab products found in correlation

Dnase 1, by Thermo Fisher Scientific (4 mentions) Hiseq 2500, by Illumina (2 mentions) C57bl 6j, by Japan SLC (1 mentions) Nebnext polya isolation module, by New England Biolabs (1 mentions) Ribo zero, by Illumina (1 mentions) Myd88, by Jackson ImmunoResearch (1 mentions) Nebnext poly a mrna magnetic isolation module, by New England Biolabs (1 mentions) Nebnext multiplex oligos for index primers set 1, by Illumina (1 mentions)

4 protocols using mavs mice

1

Genetic Knockout Mice for Immune Research

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Age- and sex-matched C57BL/6J obtained from Japan SLC, Inc. were used as WT controls. cGAS−/−, Stinggt/gt (C57BL/6J-Tmem173gt/J), and MAVS−/− mice were purchased from Jackson Laboratory (026554, 017537, and 008634 respectively). All animal experiments were performed in accordance with the regulations of the University of Tokyo Committee for Animal Care and Use and were approved by the Animal Experiment Committee of the Institute of Medical Science, the University of Tokyo.
Moriyama M., Koshiba T, & Ichinohe T. (2019). Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses. Nature Communications, 10, 4624.
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2

Profiling Nascent RNA in Mouse Brain

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Mavs+/− mice were acquired from Jackson Laboratory and crossed with Adar+/− and subsequently with Adarb1−/−, Gria2R/R (stock #008634, allele: Mavstm1Zjc). For Nascent-seq, mice of desired genotypes were sacrificed at p14, and nascent-RNA was isolated from brain tissue (Menet et al. 2012 (link)). After treatment with DNase I (Thermo Fisher Scientific), contaminating polyadenylated RNA was removed using the NEBNext poly(A) Isolation Module (New England Biolabs). Ribosomal RNA was removed using Ribo-Zero (Illumina). cDNA libraries were generated from 1 µg of nascent-RNA using NEBNext Ultra Directional RNA Library Prep kit for Illumina (New England Biolabs) and sequenced in paired-end mode with 125-bp read length on a HiSeq 2500 (Illumina).
Licht K., Kapoor U., Amman F., Picardi E., Martin D., Bajad P, & Jantsch M.F. (2019). A high resolution A-to-I editing map in the mouse identifies editing events controlled by pre-mRNA splicing. Genome Research, 29(9), 1453-1463.
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3

Immune Signaling Pathways Modulation

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C57BL/6 mice and 129 mice were obtained from Jackson and Taconic. Myd88−/−, Trif−/−, P2×7r−/−, and Mavs−/−mice were purchased from Jackson ImmunoResearch Laboratories. Tlr4−/−mice were provided by Dr. Cathryn Nagler and Dr. Haochu Huang (University of Chicago), and Tlr9−/− mice were purchased from Oriental Bioservice. Tmem173−/− (STING-deficient) mice were generated by Dr. G. Barber (University of Miami). Irf3−/− (IRF3-deficient) mice were obtained from RIKEN Bio Resource Center. The C57BL6-derived melanoma cell line B16.F10.SIY (henceforth referred to as B16.SIY) was generated as described (Blank et al., 2005 (link)). 1969 sarcomas tumor cells were gifted by Dr. Robert Schreiber (Washington University). All cells were cultured in complete DMEM media supplemented 10% heat-inactivated FCS. For measurement of type I IFN reporter activity, B16-Blue IFN reporter cells were purchased from InvivoGen and maintained according to the manufacturer’s instructions. Animals were used according to protocols approved by the IACUC of University of Chicago according to NIH guidelines for animal use.
Woo S.R., Fuertes M.B., Corrales L., Spranger S., Furdyna M.J., Leung M.Y., Duggan R., Wang Y., Barber G.N., Fitzgerald K.A., Alegre M.L, & Gajewski T.F. (2014). STING-Dependent Cytosolic DNA Sensing Mediates Innate Immune Recognition of Immunogenic Tumors. Immunity, 41(5), 830-842.
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4

RNA-seq of Mavs and Adar Mice

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Mavs+/− mice were acquired from Jackson Laboratory (stock #008634, Allele: Mavstm1Zjc). AdarΔ7-9 and Adarb1+/−; Gria2R/R were kindly provided by Dr. Peter Seeburg (Higuchi et al. 2000 (link); Hartner et al. 2004 (link)). Mice were bred using standard in-house mouse facility/FELASA guidelines. For RNA-seq, age-/sex-matched littermate mice of desired genotypes were sacrificed at 2 wk of age, the cortex was isolated, and RNA was extracted using TriFast (VWR Peqlab) and DNase I treated (Thermo Fisher Scientific) following the manufacturer's instructions. Three biological replicates were used for each genotype. To prepare RNA-seq libraries, we started with 1 µg total RNA from each sample and performed poly(A) RNA selection using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were subsequently generated from isolated poly(A) RNA using the NEBNext Ultra Directional RNA Library Prep kit for Illumina (New England Biolabs), barcoded using NEBNext Multiplex Oligos for Illumina Index Primers Set 1 (New England Biolabs), and sequenced in a paired-end mode with 125-bp read length using the HiSeq 2500 (Illumina) platform.
Kapoor U., Licht K., Amman F., Jakobi T., Martin D., Dieterich C, & Jantsch M.F. (2020). ADAR-deficiency perturbs the global splicing landscape in mouse tissues. Genome Research, 30(8), 1107-1118.
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