The A2780 cell line was purchased from American Type Culture Collection (ATCC), and SAHA was purchased from Selleckchem (Houston, TX, USA). Primary antibodies against Ac-H2A, Ac-H2B, Ac-H3, Ac-H4, HDAC2, HDAC3, HDAC4, DNMT3A, PRMT1, SUV39H1, MDMX, p53, p21WAF1/CIP1, p27Kip1, AURKB, CDC25C, GADD45A (1:1000) and Alexa Fluor 488 dye were purchased from Cell Signaling Technology (Danvers, MA, USA). The MDM2 antibody (1:500) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). β-actin (1:5000), secondary antibodies (anti-rabbit, anti-mouse), horseradish peroxidase (HRP) conjugate, and dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich (St. Louis, MO, USA). Nitrocellulose membrane (NC) (0.45 µm) was purchased from Amersham (GE Healthcare Life Sciences, Marlborough, MA, USA) and KPL LumiGlo Reserve chemiluminescent substrate was purchased from SeraCare Life Sciences (Milford, MA, USA).
Alexa fluor 488 dye
Manufactured by Cell Signaling Technology
Sourced in United States
Alexa Fluor 488 dye is a fluorescent dye used in various biological applications. It is a green-fluorescent dye with excitation and emission maxima of 495 and 519 nm, respectively. The dye can be used for labeling biomolecules, such as proteins and nucleic acids, to enable their visualization and detection in various imaging techniques.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hdac3, by Cell Signaling Technology (1 mentions)
A2780 cell line, by American Type Culture Collection (1 mentions)
β actin, by Merck Group (1 mentions)
P27kip1, by Cell Signaling Technology (1 mentions)
P21 waf1 cip1, by Cell Signaling Technology (1 mentions)
Cdc25c, by Cell Signaling Technology (1 mentions)
Gadd45a, by Cell Signaling Technology (1 mentions)
Aurkb, by Cell Signaling Technology (1 mentions)
Ac h3, by Cell Signaling Technology (1 mentions)
Ac h4, by Cell Signaling Technology (1 mentions)
Prmt1, by Cell Signaling Technology (1 mentions)
Suv39h1, by Cell Signaling Technology (1 mentions)
Mdm2 antibody, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase hrp conjugate, by Merck Group (1 mentions)
Hdac2, by Cell Signaling Technology (1 mentions)
Hdac4, by Cell Signaling Technology (1 mentions)
Dnmt3a, by Cell Signaling Technology (1 mentions)
2 protocols using alexa fluor 488 dye
1
Histone Modification Profiling in A2780 Cells
2
Cytochrome C Translocation in AgNP and CisPt Treated Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A2780 and SK-OV-3 cells were grown on coverslips at a density of 2x105 cells/mL followed by the treatment with AgNP (10 μg/ml) and CisPt (3 µg/ml: 10 μM) for 6, 12, 24h. After each time points, cells were fixed with 4% paraformaldehyde (20 min, RT). Further, cells were permeabilized with 0.3% Triton X- 100/PBS (15 min, RT) and blocked with 8% BSA/PBS (1 h, RT). Next, cells were stained with mouse monoclonal anti-cytochrome C (6H2.B4 antibody, 1:100 dilution in 1% BSA/PBS, at 4 °C, overnight) purchased from Cell Signaling Technology, Danvers, MA, USA (Cat. # 12963), followed by a secondary antibody staining (1: 1000 dilution, Alexa Fluor® 488 dye) for 2 h at room temperature purchased from Cell Signaling Technology, Danvers, MA, USA (Cat. # 4408). For nuclear staining, cells were stained with DAPI 1 mg/mL (1:10000 dilution in PBS, RT, 1 min). The cells were washed three times with PBS after all incubations. The coverslips were mounted on glass slides with fluorSave™ reagent (Cat. # 345789; Millipore: Burlington, MA, USA) and examined using a Leica DM5500B fluorescence microscope with a X-Cite 120 PC Q lamp and the images were analysed with Image J software.
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