Tamoxifen (TMX; Sigma) was prepared as 20 mg/mL stock in corn oil (Sigma) and administered by intraperitoneal (IP) injection at 10 μl/g body weight for 3 consecutive days. After which, mice were chased for 5 d or longer (in legends) before harvest or injury. Similar lineage tracing results were found with 1 M TMX washout.
Tamoxifen tmx
Manufactured by Merck Group
Sourced in United States
Tamoxifen (TMX) is a selective estrogen receptor modulator (SERM) used as a laboratory research tool. It functions by binding to and modulating the activity of estrogen receptors in cells. Tamoxifen is commonly employed in studies investigating the role of estrogen signaling in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Corn oil, by Merck Group (3 mentions)
Paclitaxel, by Merck Group (2 mentions)
Fluorescence microscope, by Leica camera (2 mentions)
Rosa ai9, by Jackson ImmunoResearch (1 mentions)
Charcoal stripped fbs, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Tamoxifen, by Instech (1 mentions)
Luzindole, by Merck Group (1 mentions)
Ficoll, by Cedarlane (1 mentions)
Hyaluronic acid, by Merck Group (1 mentions)
Chitosan cs, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
Tdtomato, by Addgene (1 mentions)
H2b egfp, by Addgene (1 mentions)
Ivis lumina series 3, by PerkinElmer (1 mentions)
Sunflower seed oil, by Merck Group (1 mentions)
29 protocols using tamoxifen tmx
1
Tamoxifen-Mediated Lineage Tracing
2
Tracing Adult Scx-lineage Cells
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Scx-CreERT2 mice were generously provided by Dr. Ronen Schweitzer. ROSA-Ai9 (#007909) mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). ROSA-Ai9 mice express the red fluorescent protein variant tdTomato following Cre-mediated recombination. Scx-CreERT2 mice were crossed to the ROSA-Ai9 strain to trace adult Scx-lineage cells (ScxAi9). Cre+ males and females were used for all studies and underwent tendon surgery at 10–12 weeks of age. For all experiments, ScxAi9 animals received three 100mg/kg i.p. tamoxifen (Tmx; #T5648, Sigma Life Sciences) injections beginning seven days prior to flexor tendon repair surgery to trace and assess the fate of adult ScxAi9 cells, while also ensuring that no subsequent labelling occurred during tendon healing by allowing for a washout period of four days. All mice were maintained under pathogen-free conditions and housed with no more than five animals per cage under standardized light-dark cycle conditions with ad libitum access to food and water. The vivarium was maintained under controlled temperature and humidity.
This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All animal procedures were approved by the University Committee on Animal Research (UCAR) at the University of Rochester.
This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All animal procedures were approved by the University Committee on Animal Research (UCAR) at the University of Rochester.
3
Establishing Cell Line Cultures for Cancer Research
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The 4T1 mouse breast cancer, LL2 mouse lung cancer, MCF-7 human breast cancer (luminal A phenotype) and MDA-MB-468 human TNBC (basal phenotype) cell lines were purchased from DS Pharma (Tokyo, Japan).18 (link) The CT26 mouse colon cancer cell line was a kind gift from Professor I. J. Fidler (MD Anderson Cancer Center). The cell lines were maintained in DMEM (Sigma Chemical, St. Louis, MO, USA) containing 10% FBS (Sigma) in a 5% CO2 atmosphere at 37°C. The following reagents were purchased: charcoal stripped FBS (Sigma), tamoxifen (TMX; Sigma), angiotensin I and II (A-I and II; Abgent, San Diego, CA, USA), angiotensinogen (ATG; Calbiochem, Darmstadt, Germany), angiotensin 1-7 (A1-7; California Peptide Research, Napa, CA, USA), cisplatin (CDDP; Alexis) and xanthenone (XTN; Alfa Aesar, Ward Hill, MA, USA).
4
Timed Pregnant Mice for NC Lineage Tracing
5
Melatonin and Calmodulin Receptor Antagonists
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Luzindole (antagonist for melatonin MEL-a receptor, 5 mg/single), Sigma (USA).
Tamoxifen (TMX) (receptor antagonist of calmodulin, 1 g/single), Sigma (USA).
Ficoll to separate and purify the blood platelets, Cedarlane, Ltd., number: CL5020-6.
CaM Rat Kit to measure the level of calmodulin in platelets of rats from each group, Wuhan Gene Biological Technology Co., Ltd.
Absolute alcohol, Hefei Sanding Chemical Co., Ltd.
6
Chitosan-Hyaluronic Acid Nanoparticles for Targeted Tamoxifen Delivery
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Chitosan (CS) [low molecular weight (MW = 50–160 kDa), 85% deacetylation degree], hyaluronic acid (HA) (MW = 100 kDa), and tamoxifen (TMX), as well as tripolyphosphate (TPP), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA. Glacial acetic acid and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were obtained from Merck, Germany. Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM) were supplied from Gibco, USA. CD44-overexpressing MCF7 breast cancer cell lines were bought from Iranian Biological Resource Center (IBRC), Tehran, Iran, which were confirmed for overexpression of the CD44 antigen. Human fibroblast (HF) (used as normal control cells in the following experiments) were purchased from the National Cell Bank of Iran (NCBI), Tehran, Iran. Double-distilled deionized water (DW) from Zolal, Iran, was used in all experiments.
7
Tamoxifen-Mediated Lineage Tracing
8
Conditional Rspo2 Ablation in Naphthalene-Induced Lung Injury
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Eight-week-old wild-type C57BL/6N or Rspo2flox/flox;CAG-CreESR male mice were injected intraperitoneally with naphthalene (275 mg/kg body weight). To conditionally ablate Rspo2 before injury, 75 mg/kg of tamoxifen (TMX; 20 mg/mL in corn oil; Sigma-Aldrich, St. Louis, MO, USA) or corn oil were injected intraperitoneally into Rspo2flox/flox;CAG-CreESR mice every 24 h for five consecutive days starting from day 10 before naphthalene injury. The lungs were harvested at different time points after injury, as indicated.
9
Genetic Targeting of Cx26 in Cochlear Cells
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All experiments were approved by the Committee of Tongji Medical College, Huazhong University of Science and Technology. Cx26f/f mice were crossed with Fgfr3iCreERT2 or Lgr5CreER mice to obtain Cx26f/f; Fgfr3iCreERT2 and Cx26f/f; Lgr5CreER mice. All mice were injected with tamoxifen (TMX, T5648-1G, Sigma-Aldrich) subcutaneously at P0 and P1 (the total dose was 1.5 mg/10 g body weight, once a day for two consecutive days) [13 (link), 36 (link)]. In Cx26f/f; Lgr5CreER mice, Cx26 of the third row of DCs was successfully knocked out [36 (link)]. The Cx26f/f; Fgfr3iCreERT2 line was used to establish a targeted DCs and PCs Cx26-null mouse model [16 (link)].
All mice were raised in the specific-pathogen-free Experimental Animal Center of Huazhong University of Science and Technology. The animals were housed at 22 ± 1 °C under a standard 12 h light/dark cycle and were allowed free access to water and a regular mouse diet.
All mice were raised in the specific-pathogen-free Experimental Animal Center of Huazhong University of Science and Technology. The animals were housed at 22 ± 1 °C under a standard 12 h light/dark cycle and were allowed free access to water and a regular mouse diet.
10
Estrogen receptor agonist and antagonist study
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E2 was purchased from Sigma Aldrich Co. (St. Louis, MO, USA) and dissolved in 5% arabic gum. The estrogen receptor antagonist tamoxifen (TMX) was purchased from Sigma Aldrich Co. (St. Louis, MO, USA), and dissolved in 10 µg/µL sesame oil. All drugs were administered intraperitoneally in a constant volume of 10 mL/kg body weight. To compare the effect of fasting and other drugs, mice were treated with either the respective vehicle or the respective drugs, E2 (15 μg/kg, ip) or TMX (15 mg/kg ip).
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