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Protocol hema 3 cell staining kit

Manufactured by Thermo Fisher Scientific
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The Protocol HEMA-3 cell staining kit is a laboratory product designed for cell staining. It provides the necessary reagents and protocols for performing Romanowsky-type staining of blood cells and other cell types.

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Lab products found in correlation

2000 research light microscope, by Nikon (2 mentions) Cytospin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Hema 3 (99 citations) Hema 3 staining kit (39 citations) Protocol Hema 3 (16 citations) PROTOCOL Hema 3 staining kit (7 citations) HEMA-3 staining (5 citations) Protocol Hema 3 kit (3 citations)

3 protocols using protocol hema 3 cell staining kit

1

Quantifying Inflammatory Cells in BALF

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The bronchoalveolar lavage fluid (BALF) of the rats was obtained for quantifying inflammatory cells. After the rats were narcotized, as mentioned in 2.1, we put the front end of a bronchoscope into the opening at the lateral segmental bronchus in the middle lobe of the right lung, injected 2 mL of 37 ℃ normal saline, and conducted lavage three times. PMNs, extracted out of BALF, were purified and centrifuged at 1000 r/min for 10 min. With the supernatant harvested, RMPI-1640 was administered to resuspend the cells. A hematocyte counter (Beckman Coulter, Inc) was utilized to count inflammatory cells in BALF. Cytospin (Thermo Fisher Scientific, Waltham, USA) was harnessed to centrifuge 100 μl of BALF onto slides. After getting dried, the slides were dyed employing the Protocol HEMA-3 Cell Staining Kit (Fisher, Pittsburg, PA).
Wang L., Yuan N., Li Y., Ma Q., Zhou Y., Qiao Z., Li S., Liu C., Zhang L., Yuan M, & Sun J. (2022). Stellate ganglion block relieves acute lung injury induced by severe acute pancreatitis via the miR-155-5p/SOCS5/JAK2/STAT3 axis. European Journal of Medical Research, 27, 231.
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2

Quantification of Inflammatory Cells in BALFs

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Quantification of inflammatory cells from BALFs was performed as described previously (21 (link)–23 (link)). In brief, BALF sample was prepared from individual animals (n = 8 mice per group). About 10 μL of each fresh BALF was used for total cell counting with a hemocytometer. The remaining BALF samples were centrifuged for 10 min at 250 x g at 4°C. The cell pellet was resuspended with 1 mL of cold sterile saline. The cells in 200 μL resuspended cell solutions were mounted on a slide by cytospin centrifugation at 1,000 rpm for 3 min. Cells were stained using the Protocol HEMA-3 cell staining kit (Fisher, Pittsburg, Pa). Macrophages/monocytes and polymorphonuclear neutrophils (PMNs) on the slides were identified and quantified by Nikon 2000 research light microscope (×200 magnification).
Yang F., Zhang J., Yang Y., Ruan F., Chen X., Guo J., Abdel-Razek O., Zuo Y.Y, & Wang G. (2020). REGULATORY ROLES OF HUMAN SURFACTANT PROTEIN B VARIANTS ON GENETIC SUSCEPTIBILITY TO PSEUDOMONAS AERUGINOSA PNEUMONIA-INDUCED SEPSIS. Shock (Augusta, Ga.), 54(4), 507-519.
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3

Quantification of Inflammatory Cells in BALF

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Bronchoalveolar lavage fluid (BALF) was prepared for quantification of inflammatory cells in a separate group of experimental animals. To perform BAL, 0.5 ml saline was instilled via trachea and then gently withdrawn, it was repeated for 3 times. Normally, a total of 1.2 mL of BALF were obtained from each mouse lung. Total cell counts in BALF were determined with a hemocytometer. The BALF samples were centrifuged at 250×g for 10 min and then Cells were resuspended in 1 ml of sterile saline (4°C). 100 µl diluted cell solution were mounted on a slide by cytospin centrifugation at 1000 rpm for 3 min. Slides were stained using the Protocol HEMA-3 cell staining kit (Fisher, Pittsburg, PA). Macrophages/monocytes and polymorphonuclear neutrophils (PMNs) in the BALF were identified and quantified by Nikon 2000 research light microscope (× 200 magnification).
Yu J., Ni L., Zhang X., Zhang J., Abdel-Razek O, & Wang G. (2019). Surfactant protein D dampens lung injury by suppressing NLRP3 inflammasome activation and NF-κB signaling in acute pancreatitis. Shock (Augusta, Ga.), 51(5), 557-568.
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