H. pylori (ATCC 43504) with the typical S shape, gram-negative rods, possessing the CagA and VacA were provided in a frozen state by ATCC. H. pylori ATCC 43504 strains were grown on tryptic soy agar with 5% sheep blood agar (BD Diagnostics) and Dent antibiotics supplement (Oxoid) at 37°C under microaerophilic conditions (Campy-Pak 273 System, BBL). RGM1 cells were incubated overnight in fresh serum- and antibiotic-free RPMI 274 medium and were infected with H. pylori at multiplicities of infection (MOI) of 100:1 for 48 h. Though we found no influence of WPE on H. pylori culture, we repeated the status of H. pylori colonization in group treated with WPE in the presence of H. pylori and H. pylori infection alone before RNA isolation for RNAseq analysis.
Dent antibiotics supplement
Manufactured by Thermo Fisher Scientific
Dent antibiotics supplement is a laboratory product designed for use in the preparation and testing of antibiotic compounds. It provides a standardized solution for researchers to evaluate the effectiveness of various antibiotics. The core function of this product is to facilitate antibiotic testing and analysis within a controlled laboratory setting.
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Lab products found in correlation
3 protocols using dent antibiotics supplement
1
Helicobacter pylori Infection Model in RGM1 Cells
2
In vitro H. pylori Infection Assay
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H. pylori (ATCC 43504) with the typical S shape, gram-negative rods, possessing the CagA and VacA were provided in a frozen state by ATCC. H. pylori ATCC 43504 strains were grown on tryptic soy agar with 5% sheep blood agar (BD Diagnostics) and Dent antibiotics supplement (Oxoid) at 37°C under microaerophilic conditions (Campy-Pak 273 System, BBL). RGM1 cells were incubated overnight in fresh serum- and antibiotic-free RPMI 274 medium and were infected with H. pylori at multiplicities of infection (MOI) of 50:1.
3
H. pylori Infection of AGS Cells
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H. pylori (ATCC® 43504TM) with the typical S shape, gram-negative rods, possessing the CagA and VacA were provided in a frozen state by ATCC. A mouse adaptive strain of H. pylori, Sydney strain 1 (SS1)40 (link) was provided by Prof. Ki-Baik Hahm of CHA University. Both strains were grown on tryptic soy agar with 5% sheep blood agar (BD Diagnostics) and Dent antibiotics supplement (Oxoid) at 37 °C under microaerophilic conditions (Campy-Pak System; BBL). AGS cells were incubated overnight in fresh serum- and antibiotic-free RPMI medium and were infected with H. pylori at multiplicities of infection (MOI) of 100:1.
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