Serum was separated from trunk blood 24 h following the LPS injection by centrifugation at 4,000 × g for 5 min and was subsequently stored at −20°C until use. Serum corticosterone levels were measured using ELISA kits (Corticosterone ELISA kit; EIAab Science Co, Ltd., Wuhan, China), according to the manufacturer's instructions. The concentrations of interleukin (IL)-1β, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-6, IL-4, IL-10, brain-derived neurotrophic factor (BDNF) and insulin-like growth factor (IGF)-1 in several brain zones were determined using commercially available ELISA assays, according to the instructions supplied by the manufacturer (Mouse IL-1β ELISA set, Mouse IFN-γ (AN-18) ELISA set, Mouse IL-4 ELISA set, Mouse IL-10 ELISA set; all purchased from BD Pharmingen™; BD Biosciences, Franklin Lakes, NJ, USA Mouse TNF-α ELISA kit, Mouse IL-6 ELISA kit, Mouse BDNF ELISA kit, Mouse IGF-1 ELISA kit; all purchased from EIAab Science Co, Ltd.).
Mouse il 4 elisa set
Manufactured by BD
Sourced in United States
The Mouse IL-4 ELISA Set is a laboratory equipment used for the quantitative determination of mouse interleukin-4 (IL-4) in biological samples. It provides the necessary components to perform an enzyme-linked immunosorbent assay (ELISA) for the detection and measurement of mouse IL-4 levels.
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Lab products found in correlation
2 protocols using mouse il 4 elisa set
1
Measurement of Serum and Brain Biomarkers
2
Serum Cytokine and Corticosterone Assay
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After the collection of stool samples, the mice were anesthetized deeply with 10% chloral hydrate before the blood was collected from the heart. The blood samples stored in tubes were left at room temperature for 30 min and then centrifuged (4,000g, 10 min, at 4°C) for serum separation. Then, the prepared serum samples were used strictly according to the manufacturer’s protocols for ELISA assays to determine the levels of IFN-γ, TNF-α, IL-6, IL-4, IL-10, and corticosterone. Mouse IFN-γ (AN-18) ELISA set, Mouse IL-4 ELISA set, and Mouse IL-10 ELISA set were purchased from BD PharmingenTM (BD Biosciences). Mouse TNF-α ELISA kit, Mouse IL-6 ELISA kit, and corticosterone ELISA kit were purchased from EIAab Science Co, Ltd.
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