Serum was collected for renal creatinine detection from day 1 to day 3 after AKI. Serum creatinine was detected by using a direct Creatinine LiquiColor kit (Stanbio Laboratory, Boerne, TX, USA). Renal pathology is examined by periodate‐Schiff (PAS) staining (Sigma‐Aldrich, 395B, St. Louis, MO, USA), formalin‐fixed kidney tissues were embedded into paraffin and sectioned (3 μm), and then followed by PAS staining. The number of tubular necrosis was scored at ×40 magnification and a total of 10 fields of cortical versions were examined and calculated as previously study described.19 , 20 , 21
Creatinine liquicolor kit
Manufactured by EKF Diagnostics
Sourced in United States
The Creatinine LiquiColor kit is a laboratory diagnostic test used to measure the concentration of creatinine in a sample. Creatinine is a waste product that is filtered out of the blood by the kidneys. The kit provides a quantitative analysis of creatinine levels, which can be used to assess kidney function.
Automatically generated - may contain errors
Lab products found in correlation
Urea nitrogen direct kit, by EKF Diagnostics (1 mentions)
Enzyme linked immunosorbent assay kit, by Exocell (1 mentions)
Mouse anti dsdna igg elisa kit, by Alpha Diagnostic (1 mentions)
Hematrue veterinary hematology analyzer, by Heska (1 mentions)
Hydroxylamine, by Merck Group (1 mentions)
Bioready 150 nm gold nanoshells, by NanoComposix (1 mentions)
4 protocols using creatinine liquicolor kit
1
Serum Creatinine and Renal Pathology in AKI
2
Biochemical Markers in Urine and Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine and blood samples were collected as previously described [13 (link)]. Urine albumin, plasma triglyceride levels, plasma urea levels, and serum creatinine levels were determined using commercial kits, an enzyme-linked immunosorbent assay kit (Exocell), a Urea Nitrogen Direct Kit (Stanbio Laboratory), a LabAssay Triglyceride ELISA Kit (Wako), and a Creatinine Liquicolor Kit (Stanbio Laboratory).
3
Autoantibodies, Urine Albumin, and Creatinine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum autoantibodies and urine albumin were measured by ELISA using the mouse anti-SSA (RO-52) ELISA kit (Signosis, Inc., Santa Clara, CA, United States), the mouse anti-dsDNA IgG ELISA Kit (Alpha Diagnostic International, San Antonio, TX, United States), and the Albuwell M ELISA kit (Ethos Biosciences Inc., Philadelphia, PA, United States). Urine creatinine was measured using a Creatinine Liquicolor kit (Stanbio Laboratory, Boerne, TX, United States). Hematocrit was measured using the HemaTrue® Veterinary Hematology Analyzer (Heska, Loveland, CA, United States).
4
Lateral Flow Assay for HE4 Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland). The reporter reagent used in the LFA was BioReady150 nm gold nanoshells by nanoComposix Inc (San Diego, California). Ethyl-N;-(3-dimethylaminopropyl) carbodiimine hydrochloride (EDC) and Sulfo-NHS chemistry was used (sigma Cat# E1769-1G, Thermo, Prod# 24510) to covalently bind the antibody to the nanoshell. Hydroxylamine (Sigma, cat# 467804) was used to quench remaining amine groups during the covalent conjugation protocol.
HE4 antigen standards were provided by Fujirebio Diagnostics Inc. (Malvern, PA). For the enzymatic creatinine reaction, we purchased a Creatinine LiquiColor kit from Stanbio (Boerne, TX). The creatinine test paper used was Whatman no. 3, purchased from Cytiva (Marlborough, MA). The dipping soy wax was purchased from Hearts & Crafts (Brooklyn, NY). Parafilm tape was purchased from Hach (Loveland, CO). Anhydrous creatinine was purchased from Thermo (Prod # C4225, lot# SLCF5841). All solutions were prepared with deionized water unless otherwise noted.
HE4 antigen standards were provided by Fujirebio Diagnostics Inc. (Malvern, PA). For the enzymatic creatinine reaction, we purchased a Creatinine LiquiColor kit from Stanbio (Boerne, TX). The creatinine test paper used was Whatman no. 3, purchased from Cytiva (Marlborough, MA). The dipping soy wax was purchased from Hearts & Crafts (Brooklyn, NY). Parafilm tape was purchased from Hach (Loveland, CO). Anhydrous creatinine was purchased from Thermo (Prod # C4225, lot# SLCF5841). All solutions were prepared with deionized water unless otherwise noted.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!