Haptoglobin was purified from 25 μL of serum using an anti-haptoglobin antibody immobilized column [26 (link)]. The purification was performed on an Alliance high performance liquid chromatography system (Waters, Milford, MA, USA) with a flow rate of 0.5 mL/min for 40 min. The haptoglobin bound fraction was eluted with a stripping buffer (0.1 mol/L glycine, pH 2.5) and neutralized with a neutralization buffer (0.1 mol/L Tris-HCl, pH 8.0). The eluted fraction (~ 3 mL) was desalted using a 4 mL YM-3 centrifugal device (Sigma-Aldrich) and then dried in a SpeedVac concentrator.
Alliance high performance liquid chromatography system
Manufactured by Waters Corporation
Sourced in United States
The Alliance high performance liquid chromatography (HPLC) system is a laboratory instrument designed for the separation, identification, and quantification of chemical compounds. It utilizes a high-pressure liquid pump to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. The interactions between the sample components and the adsorbent material cause the individual compounds to separate, which are then detected and measured by a suitable detector.
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Lab products found in correlation
2 protocols using alliance high performance liquid chromatography system
1
Haptoglobin Purification from Serum
2
HPLC Analysis of Sugar and Ethanol
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Sugar concentration (glucose and maltose) and produced ethanol were measured by an Alliance high-performance liquid chromatography system (Waters, Milford, MA, USA, Separation module e2695) coupled with autosampler and two consecutive detectors, Waters 996 photodiode array, and Hewlett Packard HP1047A RI. Empower 3.0 software was used for data collection and analysis. The analytes were separated on Hi-Plex H, 300 × 6.5 mm (Agilent technologies, Santa Clara, CA, USA) column at a flow rate of 0.5 mL/min at 40 °C. The running buffer was H2SO4 (5 mM). All beers were run through filters (Whatman, TishScientific, North Bend, OH, USA) with a pore size of 0.45 µm before being injected into the instrument.
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