The pathological sections of tumor tissues were retrieved by EDTA antigen retrieval (Solarbio), blocked by 5% BSA, followed by incubating with anti‐integrin β8 Ab (1:100, Abcam) for 4°C overnight, signal amplification staining using the ABC HRP Kit (Thermo) and counter‐staining with hematoxylin. The images were captured with microscope (Leica, Barnack).
Edta antigen retrieval
Manufactured by Solarbio
EDTA antigen retrieval is a laboratory technique used to expose epitopes (antigenic sites) on proteins that have been masked or altered during the fixation process. It involves the use of ethylenediaminetetraacetic acid (EDTA) to chelate and remove divalent cations, which can help to reverse the effects of protein cross-linking caused by formalin fixation. This process enhances the accessibility of target antigens, allowing for improved immunodetection in immunohistochemistry and other applications.
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Lab products found in correlation
2 protocols using edta antigen retrieval
1
Immunohistochemical Analysis of Integrin β8
2
Immunofluorescence Analysis of c-Myc in Tumor Tissues
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The pathological sections of tumor tissues were retrieved by EDTA antigen retrieval (Solarbio). The tumor cells were fixed with 4% paraformaldehyde and permeated by 0.5% Triton‐X100. The tissues or cell samples were then blocked by 5% BSA, followed by incubating with anti‐c‐Myc primary Ab (1:400, Abcam) for 4°C overnight, and followed by secondary Abs (1:600; Abcam). The nucleus was stained with DAPI. The immunofluorescence images were captured from FV1000 laser scanning confocal microscope (Leica, Barnack).
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