A fasting venous blood sample was taken from each participant. The samples were put into EDTA-containing vacutainer tubes and transported without delay to Komfo Anokye Teaching Hospital and refrigerated at 4°C until analysed for full blood count, haemoglobin (Hb), packed cell volume (PCV)/haematocrit, mean corpuscular volume (MCV), red cell count (RBC), and platelets using the Cell-Dyn 3700 Haematology Analyzer (Abbott Diagnostics) and also for fasting blood glucose. A diagnosis of diabetes was made if a single fasting blood glucose sample was >7.0 mmol/L or the participant was on treatment for diabetes.
Cell dyn 3700 haematology analyzer
Manufactured by Abbott
Sourced in United States
The Cell-Dyn 3700 Haematology Analyzer is a laboratory instrument used for the analysis of blood samples. It performs automated counting and differentiation of various blood cell types, including red blood cells, white blood cells, and platelets.
Automatically generated - may contain errors
3 protocols using cell dyn 3700 haematology analyzer
1
Blood Sampling and Diabetes Diagnosis
2
Hemoglobin Variant Identification in Sickle Cell Anemia
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Two millilitres (mls) of peripheral venous blood were collected in ethylene-diamine-tetra-acetic acid (EDTA) tubes from all children on hydroxyurea for at least one year at the time of enrolment into the cross-sectional survey. A complete blood count with reticulocyte count was performed using the Cell-Dyn 3700 Haematology Analyzer (Abbott Diagnostics, Lake Bluff, IL, USA). The identification of HbA, HbS, HbF and other haemoglobin variants was done using HbE (Biotec Fisher Filipo) or IEF (GE Healthcare Bio Sciences AB, Bjorktan 30,751 84 Uppsala Sweden). The identification and quantification of HbA, HbS, HbF and other variants was done using high-performance liquid chromatography (HPLC) using the Variant I Haemoglobin Testing System (Bio-Rad, Laboratories, Inc., Hercules, CA, USA). Patients with HbS and no detectable HbA were considered to have SCA. Patients with HbS and low levels of detectable HbA on HPLC or light HbA band on HbE/IEF were considered to have sickle beta plus thalassemia (HbSβ+ thal) and were excluded from the study. All tests were performed under the experimental conditions specified by the manufacturers.
3
Comprehensive Blood and Cardiac Analysis
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Haematological indices were measured, as part of the automated complete blood count (CBC), using a Cell-Dyn 3700 haematology analyzer (Abbott Diagnostics, Santa Clara, CA, USA). Biochemical analyses were performed with the Architect ci8200 chemistry analyzer (Abbott Diagnostics, Santa Clara, CA, USA).
Transthoracic echocardiography was performed for each patient using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5-3.6 MHz phased array transducer.
Transthoracic echocardiography was performed for each patient using a Vivid S3 (GE Healthcare, Milwaukee, WI, USA) with a 1.5-3.6 MHz phased array transducer.
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