Clone mih5

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Clone MIH5 is a laboratory equipment product offered by Thermo Fisher Scientific. It serves as a core function of sample preparation and analysis. The detailed specifications and intended use of this product are not available in this response.

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Lab products found in correlation

Il 12p70, by Thermo Fisher Scientific (1 mentions) Clone eh12.2h7, by BioLegend (1 mentions) Polyjet dna transfection reagent, by SignaGen (1 mentions) Clone 29e 2a3, by BioLegend (1 mentions)

2 protocols using clone mih5

Modulation of MDSC cytokine secretion

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MDSCs isolated from spleens of B16-F10 tumor-bearing mice were pretreated for 30 min on ice with 5 μg/ml control antibody (IgG) or Anti-Mouse PD-L1 (B7-H1) Functional Grade Purified antibody 5 µg/ml (clone MIH5; eBioscience; PDL1 Block) and cultured under normoxia and hypoxia for 72 h. Supernatants were collected and the secretion of IL-6, IL-10, and IL-12p70 (eBioscience) was determined by ELISA.

Noman M.Z., Desantis G., Janji B., Hasmim M., Karray S., Dessen P., Bronte V, & Chouaib S. (2014). PD-L1 is a novel direct target of HIF-1α, and its blockade under hypoxia enhanced MDSC-mediated T cell activation. The Journal of Experimental Medicine, 211(5), 781-790.

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Validating Anti-PD-1 and Anti-PD-L1 Antibodies

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To confirm specificity of commercially acquired anti-PD-1 mAb, clone J43 (eBioscience, San Diego, CA, United States) and clone EH12.2H7 (BioLegend, San Diego, CA, United States), as well as anti-PD-L1 mAb, clone MIH5 (eBioscience) and clone 29E.2A3 (BioLegend), HEK293T cells transiently transfected with recombinant woodchuck PD-1 (wcPD-1) and woodchuck PD-L1 (wcPD-L1) were examined as additional test targets. For this purpose, sequences encoding wcPD-1 and wcPD-L1 were determined, expression vectors constructed using pCDNA 3.1(+), and HEK293T cells transfected using PolyJet DNA transfection reagent (SignaGen Laboratories LLC, Gaithersburg, MD, United States). Surface and intracellular expression of the transfected protein was determined by flow cytometry and compared to non-transfected cells and an isotype control.

Corkum C.P., Wiede L.L., Ruble C.L., Qiu J., Mulrooney-Cousins P.M., Steeves M.A., Watson D.E, & Michalak T.I. (2022). Identification of antibodies cross-reactive with woodchuck immune cells and activation of virus-specific and global cytotoxic T cell responses by anti-PD-1 and anti-PD-L1 in experimental chronic hepatitis B and persistent occult hepadnaviral infection. Frontiers in Microbiology, 13, 1011070.

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